卵形鲳鲹Tf、TNFα和C-Lys的组织分布及对美人鱼发光杆菌感染的响应
研究了卵形鲳鲹(Trachinotus ovatus)中免疫相关因子转铁蛋白(Tf)、肿瘤坏死因子α(TNFα)、C-型溶菌酶(C-Lys)的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp.piscicida)后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高(76.4%);卵形鲳鲹肾脏中细菌数量从第3~第96小时一直处于下降趋势,从1.07×10^6CFU·g^-1下降到1.29×10^3CFU·g^-1。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,...
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Published in | 南方水产科学 Vol. 13; no. 2; pp. 77 - 84 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
上海海洋大学水产与生命学院,上海201306%中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300
2017
中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300 |
Subjects | |
Online Access | Get full text |
ISSN | 2095-0780 |
DOI | 10.3969/j.issn.2095-0780.2017.02.010 |
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Summary: | 研究了卵形鲳鲹(Trachinotus ovatus)中免疫相关因子转铁蛋白(Tf)、肿瘤坏死因子α(TNFα)、C-型溶菌酶(C-Lys)的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp.piscicida)后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高(76.4%);卵形鲳鲹肾脏中细菌数量从第3~第96小时一直处于下降趋势,从1.07×10^6CFU·g^-1下降到1.29×10^3CFU·g^-1。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,CLys在头肾中表达量最高。美人鱼发光杆菌感染后卵形鲳鲹肾脏中Tf表达量在第3~第12小时实验组高于对照组,其中第6小时的表达量最高,是对照组的17.99倍;TNFα在感染后的第3、第6、第24小时实验组的表达量高于对照组,第24小时组相对于对照组的表达量最高,是对照组的6.05倍。C-Lys在感染后感染组表达量均低于对照组。以上结果表明,美人鱼发光杆菌感染能促进卵形鲳鲹肾脏组织中Tf、TNFα的表达,而抑制C-Lys的表达。 |
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Bibliography: | SONG Shasha1,2 , MA Hongling1 , FENG Juan1, SU Youlu1, CHENG Changhong1 , GUO Zhixun1(1. Key Lab. of Aquatic Product Processing, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China) Trachinotus ovatus; transferrin; tumor necrosis factor alpha; C-type lysozyme; gene expression The study investigates the tissue expression of transferrin (Tf), tumor necrosis factor alpha (TNFα) and C-type lysozyme (C-Lys) in healthy Trachinotus ovatus after it was infected with Photobacterium damselae subsp. piscicida in kidney. The results show that after infection, the accumulative mortalities of T.ovatus reached the maximumin 48 h (76.4%); and the colony of P.damselae in T.ovatus′ kidney continued to decline in 3~96 h from 1.07×10^6 CFU·g^-1 to 1.29 ×10^3 CFU·g^-1. The Tf and TNFα expressions were the highest in the liver, and the C-Lys expression was the highest in |
ISSN: | 2095-0780 |
DOI: | 10.3969/j.issn.2095-0780.2017.02.010 |