纳米金颗粒/麦尔多拉蓝溶胶的合成及NADH传感器应用
1引言 脱氢酶是氧化还原酶中为数最多的一类酶,目前已知超过300种脱氢酶使用氧化型烟酰胺腺嘌呤二核苷酸（NAD＋）作为辅酶。在这些酶和辅酶协同作用催化底物时,NAD＋从底物接受电子被还原成还原型烟酰胺腺嘌呤二核苷酸（NADH）。...
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| Published in | 分析化学 Vol. 39; no. 12; pp. 1934 - 1935 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
吉林大学药学院,长春,130021%吉林大学中日联谊医院,长春,130033
2011
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2011.01934 |
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| Abstract | 1引言
脱氢酶是氧化还原酶中为数最多的一类酶,目前已知超过300种脱氢酶使用氧化型烟酰胺腺嘌呤二核苷酸（NAD＋）作为辅酶。在这些酶和辅酶协同作用催化底物时,NAD＋从底物接受电子被还原成还原型烟酰胺腺嘌呤二核苷酸（NADH）。 |
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| AbstractList | 1引言
脱氢酶是氧化还原酶中为数最多的一类酶,目前已知超过300种脱氢酶使用氧化型烟酰胺腺嘌呤二核苷酸（NAD＋）作为辅酶。在这些酶和辅酶协同作用催化底物时,NAD＋从底物接受电子被还原成还原型烟酰胺腺嘌呤二核苷酸（NADH）。 R346 |
| Author | 张晓红 陈丽波 魏仲航 |
| AuthorAffiliation | 吉林大学药学院,长春130021 吉林大学中日联谊医院,长春130033 |
| AuthorAffiliation_xml | – name: 吉林大学药学院,长春,130021%吉林大学中日联谊医院,长春,130033 |
| Author_FL | CHEN Li-Bo ZHANG Xiao-Hong WEI Zhong-Hang |
| Author_FL_xml | – sequence: 1 fullname: ZHANG Xiao-Hong – sequence: 2 fullname: CHEN Li-Bo – sequence: 3 fullname: WEI Zhong-Hang |
| Author_xml | – sequence: 1 fullname: 张晓红 陈丽波 魏仲航 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3724/SP.J.1096.2011.01934 |
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| DocumentTitleAlternate | Synthesis and Application Gold Nanopaticles/Meldola′s Blue Colloids as NADH Biosensors |
| DocumentTitle_FL | Synthesis and Application Gold Nanopaticles/Meldola's Blue Colloids as NADH Biosensors |
| EndPage | 1935 |
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| Notes | This study reports a method for facile synthesis of gold nanoparticles/Eastman AQ55D/meldola′s blue（AuNPs/Eastman AQ55D/MDB） colloids.Cation exchange resin Eastman AQ55D can not only immobilize the redox mediator MDB by ion exchange interaction,but also serve as the protectant and stabilizer of AuNPs.Due to remarkable synergistic effect of AuNPs and MDB,the electrode modified with the AuNPs/Eastman AQ55D/MDB colloids apparently reduced the activation energy of Nico tina mide-adenine dinucleotide（NADH） oxidation in the electrode surface.Chronoamperometry on NADH biosensor showed that the linear range for fast detection of NADH was 0.1 to 1.1 mmol/L and the sensitivity was 70 mA/（mol/L）.Facile synthesis of AuNPs/Eastman AQ55D/MDB composite films with high stability,high sensitivity and high reproducibility is promising for the reliable and durable NADH biosensors. 22-1125/O6 Gold nanoparticles; Meldola′ blue; Eastman AQ55D; Nicotinamide-adenine dinucleotide ZHANG Xiao-Hong,CHEN Li-Bo,WEI Zhong-Hang （School of Phar |
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| PublicationTitle | 分析化学 |
| PublicationTitleAlternate | Chinese Journal of Analytical Chemistry |
| PublicationTitle_FL | Chinese Journal of Analytical Chemistry |
| PublicationYear | 2011 |
| Publisher | 吉林大学药学院,长春,130021%吉林大学中日联谊医院,长春,130033 |
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| Snippet | 1引言
脱氢酶是氧化还原酶中为数最多的一类酶,目前已知超过300种脱氢酶使用氧化型烟酰胺腺嘌呤二核苷酸（NAD＋）作为辅酶。在这些酶和辅酶协同作用催化底物时,NAD＋从底物... R346 |
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| StartPage | 1934 |
| SubjectTerms | NADH 传感器应用 合成 氧化还原酶 溶胶 纳米金颗粒 还原型烟酰胺腺嘌呤二核苷酸 麦尔多拉蓝 |
| Title | 纳米金颗粒/麦尔多拉蓝溶胶的合成及NADH传感器应用 |
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