MicroCT-Based Virtual Histology Evaluation of Preclinical Medulloblastoma
Purpose The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. Procedures All animal procedures were conducted in...
Saved in:
| Published in | Molecular imaging and biology Vol. 13; no. 3; pp. 493 - 499 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
New York
Springer-Verlag
01.06.2011
Springer Nature B.V |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1536-1632 1860-2002 1860-2002 |
| DOI | 10.1007/s11307-010-0372-3 |
Cover
| Abstract | Purpose
The purpose of this paper is to validate a rapid and cost-effective
ex vivo
technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer.
Procedures
All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered
Patched1, p53
mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning.
Results
Nine-micrometer resolution
ex vivo
micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (
R
= 0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (
p
= 0.02 and
p
= 0.04, respectively). The
ex vivo
microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm
3
for 9-μm isometric resolution microCT vs. 5,416,800 μm
3
for 400 × 111 × 122 μm resolution MRI) at a 28% cost savings ($400 vs. $555 per animal).
Conclusion
The
ex vivo
, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform
in vivo
imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both
in vivo
MRI and
ex vivo
microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma. |
|---|---|
| AbstractList | The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer.PURPOSEThe purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer.All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning.PROCEDURESAll animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning.Nine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (R = 0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (p = 0.02 and p = 0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm³ for 9-μm isometric resolution microCT vs. 5,416,800 μm³ for 400 × 111 × 122 μm resolution MRI) at a 28% cost savings ($400 vs. $555 per animal).RESULTSNine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (R = 0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (p = 0.02 and p = 0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm³ for 9-μm isometric resolution microCT vs. 5,416,800 μm³ for 400 × 111 × 122 μm resolution MRI) at a 28% cost savings ($400 vs. $555 per animal).The ex vivo, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma.CONCLUSIONThe ex vivo, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma. The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning. Nine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (R=0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (p=0.02 and p=0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm^sup 3^ for 9-μm isometric resolution microCT vs. 5,416,800 μm^sup 3^ for 400×111×122 μm resolution MRI) at a 28% cost savings ( 400 vs . 555 per animal). The ex vivo, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma.[PUBLICATION ABSTRACT] Purpose The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. Procedures All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning. Results Nine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes ( R = 0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice ( p = 0.02 and p = 0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm 3 for 9-μm isometric resolution microCT vs. 5,416,800 μm 3 for 400 × 111 × 122 μm resolution MRI) at a 28% cost savings ($400 vs. $555 per animal). Conclusion The ex vivo , en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma. The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning. Nine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (R = 0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (p = 0.02 and p = 0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 μm³ for 9-μm isometric resolution microCT vs. 5,416,800 μm³ for 400 × 111 × 122 μm resolution MRI) at a 28% cost savings ($400 vs. $555 per animal). The ex vivo, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma. Purpose: The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. Procedures: All animal procedures were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas Health Science Center at San Antonio. MRI imaging was performed on 6-week-old, bortezomib-treated genetically engineered Patched1, p53 mice that recapitulate the characteristics of human medulloblastoma. After MRI scans, the same mice were euthanized to collect brain or spine samples for virtual histology staining followed by microCT scanning. Results: Nine-micrometer resolution ex vivo micro X-ray computed tomography (microCT)-based virtual histology images were qualitatively reflective of high-field live animal images obtained with magnetic resonance imaging (MRI) and histopathology. Cerebellar volumes on microCT-based virtual histology correlated closely with MRI cerebellar volumes (R=0.998). MRI and microCT-based virtual histology both indicated a significant difference between cerebellar volumes of untreated and treated mice (p=0.02 and p=0.04, respectively). The ex vivo microCT method also allowed a 7,430-fold improvement in voxel resolution (voxel volume of 729 mu m super(3) for 9- mu m isometric resolution microCT vs. 5,416,800 mu m super(3) for 400111122 mu m resolution MRI) at a 28% cost savings ($400 vs. $555 per animal). Conclusion: The ex vivo, en bloc technique of microCT-based virtual histology matched MRI in reflecting histopathology. MicroCT-based virtual histology proved to be a more cost-effective technique and less labor-intensive. On the other hand, MRI provides ability to perform in vivo imaging, faster scanning and lower radiation dose by sacrificing the spatial resolution. Thus, both in vivo MRI and ex vivo microCT-based virtual histology are effective means of quantitatively evaluating therapeutic response in preclinical models of cerebellar tumors including the childhood cancer, medulloblastoma. |
| Author | Ohshima-Hosoyama, Sachiko Brady, Michelle M. McCarthy, Steven D. Giles, Francis J. Green, Dustin P. Sledz, Timothy J. Blackman, Barron A. Michalek, Joel E. Rubin, Brian P. Zarzabal, Lee Ann Keller, Charles Prajapati, Suresh I. Duong, Timothy Tatiparthy, Arun K. Kilcoyne, Aoife Samano, Aislynn K. |
| AuthorAffiliation | 3 Epidemiology and Biostatistics, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA 7 Cellular and Structural Biology, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA 8 Pediatrics University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA 1 Greehey Children’s Cancer Research Institute, University of Texas Health Science Center, 8403 Floyd Curl Drive, MC-7784 San Antonio, TX 78229, USA 6 Department of Anatomic Pathology, Cleveland Clinic, Taussig Cancer Center and the Lerner Research Institute, Cleveland, OH, USA 2 Department of Medicine, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA 4 Microphotonics Inc., Allentown, PA, USA 5 Research Imaging Institute, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA |
| AuthorAffiliation_xml | – name: 2 Department of Medicine, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA – name: 6 Department of Anatomic Pathology, Cleveland Clinic, Taussig Cancer Center and the Lerner Research Institute, Cleveland, OH, USA – name: 3 Epidemiology and Biostatistics, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA – name: 7 Cellular and Structural Biology, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA – name: 5 Research Imaging Institute, University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA – name: 8 Pediatrics University of Texas Health Science Center, 7703 Floyd Curl, San Antonio, TX 78229, USA – name: 4 Microphotonics Inc., Allentown, PA, USA – name: 1 Greehey Children’s Cancer Research Institute, University of Texas Health Science Center, 8403 Floyd Curl Drive, MC-7784 San Antonio, TX 78229, USA |
| Author_xml | – sequence: 1 givenname: Suresh I. surname: Prajapati fullname: Prajapati, Suresh I. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 2 givenname: Aoife surname: Kilcoyne fullname: Kilcoyne, Aoife organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center, Department of Medicine, University of Texas Health Science Center – sequence: 3 givenname: Aislynn K. surname: Samano fullname: Samano, Aislynn K. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 4 givenname: Dustin P. surname: Green fullname: Green, Dustin P. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 5 givenname: Steven D. surname: McCarthy fullname: McCarthy, Steven D. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 6 givenname: Barron A. surname: Blackman fullname: Blackman, Barron A. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 7 givenname: Michelle M. surname: Brady fullname: Brady, Michelle M. organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 8 givenname: Lee Ann surname: Zarzabal fullname: Zarzabal, Lee Ann organization: Epidemiology and Biostatistics, University of Texas Health Science Center – sequence: 9 givenname: Arun K. surname: Tatiparthy fullname: Tatiparthy, Arun K. organization: Microphotonics Inc – sequence: 10 givenname: Timothy J. surname: Sledz fullname: Sledz, Timothy J. organization: Microphotonics Inc – sequence: 11 givenname: Timothy surname: Duong fullname: Duong, Timothy organization: Research Imaging Institute, University of Texas Health Science Center – sequence: 12 givenname: Sachiko surname: Ohshima-Hosoyama fullname: Ohshima-Hosoyama, Sachiko organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center – sequence: 13 givenname: Francis J. surname: Giles fullname: Giles, Francis J. organization: Department of Medicine, University of Texas Health Science Center – sequence: 14 givenname: Joel E. surname: Michalek fullname: Michalek, Joel E. organization: Epidemiology and Biostatistics, University of Texas Health Science Center – sequence: 15 givenname: Brian P. surname: Rubin fullname: Rubin, Brian P. organization: Department of Anatomic Pathology, Cleveland Clinic, Taussig Cancer Center and the Lerner Research Institute – sequence: 16 givenname: Charles surname: Keller fullname: Keller, Charles email: keller@ohsu.edu organization: Greehey Children’s Cancer Research Institute, University of Texas Health Science Center, Cellular and Structural Biology, University of Texas Health Science Center, Pediatrics University of Texas Health Science Center |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20617390$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kc1u1DAURi1URNuBB2CDIjawMdzrn9jZIMGo0EqtYFHYWo7jDK48cbGTSn17XKYUqERXsZTzfb6-55DsTWnyhDxHeIMA6m1B5KAoIFDgilH-iBygboEyALZXz5K3FFvO9slhKRcAqJDxJ2SfQYuKd3BATs6Cy2l9Tj_Y4ofmW8jzYmNzHMqcYtpcN0dXNi52Dmlq0th8yd7FMAVXmTM_LDGmPtrKbu1T8ni0sfhnt98V-frx6Hx9TE8_fzpZvz-lTrZqphKlU51gAsA60HbsPUrrOWohhYdBiFbano2dduA8jMwy5qQC3rd6cNLyFXm3671c-q0fnJ_mbKO5zGFr87VJNph__0zhu9mkKyOUAt2pWvDqtiCnH4svs9mG4nyMdvJpKUa3ErXSdbcr8vpBEnWHuu5bQEVf3kMv0pKnuojaJzR0kokKvfh79LuZf-uoAO6A6qSU7Mc7BMHcKDc75aYqNzfKDa8ZdS_jwvxLWH19iA8m2S5Z6i3Txuc_M_8_9BPXKr7N |
| CitedBy_id | crossref_primary_10_1002_advs_202201723 crossref_primary_10_1107_S1600577518005489 crossref_primary_10_4236_ojmi_2015_53016 crossref_primary_10_1016_j_jneumeth_2019_108416 crossref_primary_10_1111_jmi_12543 crossref_primary_10_1007_s11060_011_0619_0 crossref_primary_10_1038_nprot_2017_101 crossref_primary_10_1097_MPH_0b013e3182309fe4 crossref_primary_10_1007_s11307_018_1246_3 crossref_primary_10_1159_000398781 |
| Cites_doi | 10.1097/00005072-199103000-00005 10.1371/journal.pgen.0020061 10.1148/rg.236035168 10.1002/pbc.21968 10.1161/CIRCULATIONAHA.108.829531 10.1182/blood-2002-08-2543 |
| ContentType | Journal Article |
| Copyright | Academy of Molecular Imaging and Society for Molecular Imaging 2010 Academy of Molecular Imaging and Society for Molecular Imaging 2011 |
| Copyright_xml | – notice: Academy of Molecular Imaging and Society for Molecular Imaging 2010 – notice: Academy of Molecular Imaging and Society for Molecular Imaging 2011 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QO 7RV 7X7 7XB 88E 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. KB0 L6V LK8 M0S M1P M7P M7S NAPCQ P64 PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS 7X8 5PM |
| DOI | 10.1007/s11307-010-0372-3 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Biotechnology Research Abstracts Nursing & Allied Health Database Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Technology Collection Natural Science Collection ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection (ProQuest) Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Nursing & Allied Health Database (Alumni Edition) ProQuest Engineering Collection Biological Sciences ProQuest Health & Medical Collection Medical Database Biological Science Database Engineering Database Nursing & Allied Health Premium Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering collection MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest Central Student Technology Collection Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection ProQuest Engineering Collection Health Research Premium Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Nursing & Allied Health Source ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Materials Science & Engineering Collection ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic ProQuest Central Student MEDLINE Engineering Research Database |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1860-2002 |
| EndPage | 499 |
| ExternalDocumentID | PMC4770897 2337684471 20617390 10_1007_s11307_010_0372_3 |
| Genre | Validation Study Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: NINDS NIH HHS grantid: R01 NS045879 |
| GroupedDBID | --- --K -5E -5G -BR -EM -Y2 -~C .86 .VR 04C 06C 06D 0R~ 0VY 123 1B1 1N0 203 29M 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 3V. 4.4 406 408 409 40D 40E 53G 5VS 67Z 6NX 7RV 7X7 88E 8AO 8FE 8FG 8FH 8FI 8FJ 8UJ 95- 95. 95~ 96X AAAVM AABHQ AACDK AAEDT AAHNG AAIAL AAJBT AAJKR AALRI AANXM AANZL AAQFI AARHV AARTL AASML AATNV AATVU AAUYE AAWCG AAWTL AAXUO AAYIU AAYQN AAYTO AAYZH ABAKF ABBBX ABBXA ABDZT ABECU ABFTV ABHLI ABHQN ABIPD ABJCF ABJNI ABJOX ABKCH ABKTR ABMNI ABMQK ABNWP ABPLI ABQBU ABQSL ABSXP ABTEG ABTKH ABTMW ABULA ABUWG ABWNU ABWVN ABXPI ACAOD ACBXY ACDTI ACGFS ACHSB ACHXU ACIUM ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPIV ACPRK ACRPL ACSNA ACZOJ ADBBV ADHHG ADHIR ADINQ ADKNI ADKPE ADMUD ADNMO ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEFQL AEGAL AEGNC AEJHL AEJRE AEKMD AEMSY AENEX AEOHA AEPYU AESKC AETLH AEVLU AEXYK AFBBN AFKRA AFLOW AFQWF AFRAH AFWTZ AFZKB AGAYW AGDGC AGJBK AGMZJ AGQEE AGQMX AGRDE AGRTI AGWIL AGWZB AGYKE AHAVH AHBYD AHIZS AHKAY AHMBA AHSBF AHYZX AIAKS AIGIU AIIXL AILAN AITGF AJBLW AJRNO AJZVZ AKMHD ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARMRJ ASPBG AVWKF AXYYD AZFZN B-. BA0 BBNVY BDATZ BENPR BGLVJ BGNMA BHPHI BKEYQ BMSDO BPHCQ BSONS BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 EBD EBLON EBS EIHBH EIOEI EJD EMOBN EN4 ESBYG EX3 F5P FDB FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GXS H13 HCIFZ HF~ HG5 HG6 HLICF HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ IMOTQ IWAJR IXC IXD IXE IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KPH L6V LK8 LLZTM M1P M41 M4Y M7P M7S MA- N2Q NAPCQ NB0 NPVJJ NQ- NQJWS NU0 O9- O93 O9I O9J OAM P9S PF0 PQQKQ PROAC PSQYO PT4 PTHSS Q2X QOR QOS R89 R9I RIG RNS ROL RPX RPZ RRX RSV S16 S1Z S27 S37 S3B SAP SDH SEW SHX SISQX SJYHP SMD SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZ9 SZN T13 TSG TSK TSV TT1 TUC TUS U2A U9L UG4 UHS UKHRP UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WJK WK8 WOW YLTOR Z45 Z7U Z7X Z82 Z83 Z87 ZMTXR ZOVNA ~KM AAPKM AAYXX ABBRH ABDBE ABFSG ACSTC ACVFH ADCNI ADHKG AEUPX AEZWR AFDZB AFHIU AFOHR AFPUW AGQPQ AHPBZ AHWEU AIGII AIXLP ATHPR AYFIA CITATION PHGZM PHGZT CGR CUY CVF ECM EIF NPM 7QO 7XB 8FD 8FK ABRTQ AZQEC DWQXO FR3 GNUQQ K9. P64 PJZUB PKEHL PPXIY PQEST PQGLB PQUKI PRINS PUEGO 7X8 5PM |
| ID | FETCH-LOGICAL-c567t-515c7942400ac08afbe15ae318454e0d4465ab2f98c0ce0f2a22c5703b68dc5a3 |
| IEDL.DBID | 7X7 |
| ISSN | 1536-1632 1860-2002 |
| IngestDate | Thu Aug 21 18:32:57 EDT 2025 Thu Sep 04 18:19:48 EDT 2025 Fri Sep 05 14:57:27 EDT 2025 Tue Sep 16 13:11:54 EDT 2025 Thu Apr 03 07:09:44 EDT 2025 Tue Jul 01 01:23:58 EDT 2025 Thu Apr 24 23:03:28 EDT 2025 Fri Feb 21 02:36:52 EST 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | MicroCT MRI Medulloblastoma Imaging Virtual histology Therapeutics |
| Language | English |
| License | http://www.springer.com/tdm |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c567t-515c7942400ac08afbe15ae318454e0d4465ab2f98c0ce0f2a22c5703b68dc5a3 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3 |
| OpenAccessLink | http://doi.org/10.1007/s11307-010-0372-3 |
| PMID | 20617390 |
| PQID | 864809524 |
| PQPubID | 55372 |
| PageCount | 7 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_4770897 proquest_miscellaneous_865187811 proquest_miscellaneous_1891886040 proquest_journals_864809524 pubmed_primary_20617390 crossref_primary_10_1007_s11307_010_0372_3 crossref_citationtrail_10_1007_s11307_010_0372_3 springer_journals_10_1007_s11307_010_0372_3 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2011-06-01 |
| PublicationDateYYYYMMDD | 2011-06-01 |
| PublicationDate_xml | – month: 06 year: 2011 text: 2011-06-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | New York |
| PublicationPlace_xml | – name: New York – name: United States |
| PublicationTitle | Molecular imaging and biology |
| PublicationTitleAbbrev | Mol Imaging Biol |
| PublicationTitleAlternate | Mol Imaging Biol |
| PublicationYear | 2011 |
| Publisher | Springer-Verlag Springer Nature B.V |
| Publisher_xml | – name: Springer-Verlag – name: Springer Nature B.V |
| References | Taniguchi, Cho, Arenkiel (CR3) 2009; 53 Johnson, Hansen, Wu (CR5) 2006; 2 Koeller, Rushing (CR1) 2003; 23 Martinez, Prajapati, Estrada (CR6) 2009; 120 Hideshima, Mitsiades, Akiyama (CR8) 2003; 101 CR4 Palombella, Dutcher (CR7) 1998; 117 Roberts, Lynch, Jones, Hart (CR2) 1991; 50 372_CR4 T Hideshima (372_CR8) 2003; 101 372_CR7 E Taniguchi (372_CR3) 2009; 53 JT Johnson (372_CR5) 2006; 2 HG Martinez (372_CR6) 2009; 120 KK Koeller (372_CR1) 2003; 23 RO Roberts (372_CR2) 1991; 50 28389781 - Mol Imaging Biol. 2017 Jun;19(3):483. doi: 10.1007/s11307-017-1079-5 |
| References_xml | – volume: 50 start-page: 134 year: 1991 end-page: 144 ident: CR2 article-title: Medulloblastoma: a population-based study of 532 cases publication-title: J Neuropathol Exp Neurol doi: 10.1097/00005072-199103000-00005 – volume: 2 start-page: e61 year: 2006 ident: CR5 article-title: Virtual histology of transgenic mouse embryos for high-throughput phenotyping publication-title: PLoS Genet doi: 10.1371/journal.pgen.0020061 – ident: CR4 – volume: 23 start-page: 1613 year: 2003 end-page: 1637 ident: CR1 article-title: From the archives of the AFIP: medulloblastoma: a comprehensive review with radiologic-pathologic correlation publication-title: Radiographics doi: 10.1148/rg.236035168 – volume: 53 start-page: 136 year: 2009 end-page: 144 ident: CR3 article-title: Bortezomib reverses a post-translational mechanism of tumorigenesis for patched1 haploinsufficiency in medulloblastoma publication-title: Pediatr Blood Cancer doi: 10.1002/pbc.21968 – volume: 120 start-page: 821 year: 2009 end-page: 822 ident: CR6 article-title: Images in cardiovascular medicine: microscopic computed tomography-based virtual histology for visualization and morphometry of atherosclerosis in diabetic apolipoprotein e mutant mice publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.108.829531 – volume: 117 start-page: 455 issue: 2 year: 1998 end-page: 64 ident: CR7 article-title: Identification of the gene encoding the tryptophan synthase beta-subunit from Chlamydomonas reinhardtii publication-title: Plant Physiol. 1998 Jun – volume: 101 start-page: 1530 issue: 4 year: 2003 end-page: 1534 ident: CR8 article-title: Molecular mechanisms mediating antimyeloma activity of proteasome inhibitor PS-341 publication-title: Blood doi: 10.1182/blood-2002-08-2543 – volume: 53 start-page: 136 year: 2009 ident: 372_CR3 publication-title: Pediatr Blood Cancer doi: 10.1002/pbc.21968 – volume: 2 start-page: e61 year: 2006 ident: 372_CR5 publication-title: PLoS Genet doi: 10.1371/journal.pgen.0020061 – volume: 101 start-page: 1530 issue: 4 year: 2003 ident: 372_CR8 publication-title: Blood doi: 10.1182/blood-2002-08-2543 – ident: 372_CR4 – volume: 50 start-page: 134 year: 1991 ident: 372_CR2 publication-title: J Neuropathol Exp Neurol doi: 10.1097/00005072-199103000-00005 – ident: 372_CR7 – volume: 120 start-page: 821 year: 2009 ident: 372_CR6 publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.108.829531 – volume: 23 start-page: 1613 year: 2003 ident: 372_CR1 publication-title: Radiographics doi: 10.1148/rg.236035168 – reference: 28389781 - Mol Imaging Biol. 2017 Jun;19(3):483. doi: 10.1007/s11307-017-1079-5 |
| SSID | ssj0017123 |
| Score | 1.978629 |
| Snippet | Purpose
The purpose of this paper is to validate a rapid and cost-effective
ex vivo
technique, microCT-based virtual histology, as an alternative to MRI... The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for... Purpose: The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI... |
| SourceID | pubmedcentral proquest pubmed crossref springer |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 493 |
| SubjectTerms | Animals Boronic Acids - pharmacology Boronic Acids - therapeutic use Bortezomib Cerebellum - drug effects Cerebellum - pathology Imaging Magnetic Resonance Imaging Medicine Medicine & Public Health Medulloblastoma - drug therapy Medulloblastoma - pathology Mice Organ Size - drug effects Pyrazines - pharmacology Pyrazines - therapeutic use Radiology Research Article Tumor Burden - drug effects User-Computer Interface X-Ray Microtomography - methods |
| SummonAdditionalLinks | – databaseName: SpringerLink Journals (ICM) dbid: U2A link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1La9wwEB7ygJJLSZM0dTYNCuTUIJAlWZaP25CQBjbkkC25GVm2aKCxy-7m_3fGa3vZ5gEF3zQS9oxGM57HJ4AzgTYAHyrFCZLrEErusrjgzvlKmVBYUVBoYHJrrqf65iF56Pq45321e5-SbE_qVbNbrNoyScGFSiVXm7CdEJwUbuKpHA-pgzRu73RDTTYcnQ3ZpzJfW2LdGL3wMF8WSv6TLW2N0NUufOy8RzZeivsTbFT1HnyYdPnxffgxofq6i3v-HY1TyX4-zqg9hLVYIBQ-Z5cDuDdrArvD467rjGS4CP6NNgV604vmyR3A9Ory_uKad5clcJ-YdMHRL_GoW1QS6rywLhRVnDiKcOpEV6IkYDRXyJBZL3wlgnRSeoLfKowtfeLUZ9iqm7r6AsxkJjbOGx-U1joJmVYm0aXSWdC-jEUEouda7jskcbrQ4ne-wkAmRufI6JwYnasIvg1T_ixhNN4jHvWiyDuNmufWaIvuoNQRnA6jqAqU33B11TzP89hmsbUGj6UI2Bs01uDWpO7aCA6Xsh3eR5I3pzKcnK5JfSAgJO71kfrxV4vIrdNU2CyN4LzfH6sXf_Mzj_6LegQ7y3A2BYCOYWsxe66-oj-0KE7a_f8XzP8Aqw priority: 102 providerName: Springer Nature |
| Title | MicroCT-Based Virtual Histology Evaluation of Preclinical Medulloblastoma |
| URI | https://link.springer.com/article/10.1007/s11307-010-0372-3 https://www.ncbi.nlm.nih.gov/pubmed/20617390 https://www.proquest.com/docview/864809524 https://www.proquest.com/docview/1891886040 https://www.proquest.com/docview/865187811 https://pubmed.ncbi.nlm.nih.gov/PMC4770897 |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1ba9RAFD7UFsQX0XqL1RLBJ2VwbplMnmS37LYquxTpyvoUJpMMFtqkdrf_33OyScpaWgjJw8yEuc835_IdgI8czwB8yBQnSKZDKJnLRMGc85UyobC8INHAbG5OFvr7Mll2tjmrzqyy3xPbjbpsPMnIv1ijLcIBqb9e_WUUNIqUq10EjUewJxCIUOSGdDnct0Qq2uhuuKYNQ9ghe6Vm6zknVGtzyRlXqWRq-1i6gzXvmkz-pzdtj6PpM3ja4ch4tBn457BT1fvweNZpyl_AtxlZ2h2dsTEeU2X86_yaHEXilhWEBOnxZKD5jpsQn-LG1_lIxvgTvJc2BeLqdXPpXsJiOjk7OmFd2ATmE5OuGSIUj6uMjEOd59aFohKJI1mnTnTFS6JIc4UMmfXcVzxIJ6UnIq7C2NInTr2C3bqpqzcQm8wI47zxQWmtk5BpZRJdKp0F7UvBI-B9r-W-4xSn0BYX-S0bMnV0jh2dU0fnKoJPQ5GrDaHGQ5kP-qHIu7W1yoeZEMGHIRUXBWk6XF01N6tc2ExYa3CDiiC-J481OEnJzzaC15uxHeojCdepDAunW6M-ZCBO7u2U-vxPy82t05TbLI3gcz8_bit-bzPfPtjMA3iyEWST6Ocd7K6vb6r3iITWxWE73_Ftp8eHsDeajsdz-h7__jHB73gyP_2JqQs5-gcOpwpU |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB6VrQRcEG9CeQQJLiALx3Yc51AhWrbapd1Vhbaot-A4sajUJqW7FeLH8d-YySaplqq9VcotduSMxzPjeXwD8JajDsCHUnG8YMr7gtk0ypm1rpTa54bn5BqYTPXoQH09jA_X4G9XC0NplZ1MbAR1UTvykX80Whk0B4T6dPqLUdMoCq52HTRs21mh2GwQxtq6jt3yz2-8wc03x19wu98JsTOcbY9Y22SAuVgnC4b63CFPUiqlddxYn5dRbMkzqGJV8oIAxWwufGocdyX3wgrhCLYq16ZwsZX43Vuwrsh_MoD1reF0_1sfxkiipr8cShXN0PARXVi1qd2LZJP1yRmXiWByVTFesnYvJ23-F7ltFOLOfbjXWrLh5yXrPYC1snoItydtrP4RjCeU67c9Y1uoKIvw-9EZlaqEDS4JufLDYQ80HtY-3EfR21ZphvgRvBnXOVr2i_rEPoaDG6HpExhUdVU-g1CnOtLWaeelUir2qZI6VoVUqVeuiHgAvKNa5lpUc2qucZxd4DEToTMkdEaEzmQA7_spp0tIj-sGb3RbkbWne571vBjAm_4tHkuKtdiqrM_nWWTSyBiNIjKA8IoxRiOvUqVvAE-Xe9uvR5BlKVOcnKzsej-AUMFX31RHPxt0cJUk3KRJAB86_rhY-JW_-fza33wNd0azyV62N57ubsDdpVudHFEvYLA4Oy9fol22yF-13B_Cj5s-cP8A4HpG6w |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwEB61i4R6gb4J9OFKPbUyOLbjOEdKdwulizhARU-p48QC0SaIzV766-vJa7VQKlWVcvNDfsx4JvP4BuAt8zLAfxiK4ziVzuXUJGFGjbGFUC7TLEPTwPRI7Z_Kz2fRWVfndNZHu_cuyTanAVGaynrnKnc7i8S3UDQhk4wyEXMq7sOKxBISI1jZ_fTtcDw4EuKwqfDm-VpRr3rw3rH5p0mWRdMtffN22OQN32kjkibr8L3fTBuJcrk9r7Nt--sGzuN_7PYhrHXqKtlt6esR3CvKx7A67RzyT-BgigF9eyf0g5eGOfl6cY35KKQBH0F7PRkPaOKkcuTYv69dKibxk_jf3yrz6ntd_TRP4XQyPtnbp111BmojFdfUK0LWMzPGoBrLtHFZEUYGTaoykgXLEYnNZNwl2jJbMMcN5xbxvjKlcxsZ8QxGZVUWG0BUokJlrLJOSCkjl0ihIpkLmThp85AFwPqLSW0HXY4VNH6kC9BlPKLUH1GKR5SKAN4NQ65a3I6_dd7qbzvtWHiWaiW11z-5DODN0Op5Dx0qpiyq-SwNdRJqrfw7GAC5o49WnhcwnTeA5y35DOvhqD6KxA-Olwhr6IDQ38st5cV5AwEu45jpJA7gfU89i4Xfuc3Nf-r9GlaPP07SLwdHh1vwoDWlo_HpBYzq63nx0utidfaq47ff4a8pJQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=MicroCT-Based+Virtual+Histology+Evaluation+of+Preclinical+Medulloblastoma&rft.jtitle=Molecular+imaging+and+biology&rft.au=Prajapati%2C+Suresh+I.&rft.au=Kilcoyne%2C+Aoife&rft.au=Samano%2C+Aislynn+K.&rft.au=Green%2C+Dustin+P.&rft.date=2011-06-01&rft.issn=1536-1632&rft.eissn=1860-2002&rft.volume=13&rft.issue=3&rft.spage=493&rft.epage=499&rft_id=info:doi/10.1007%2Fs11307-010-0372-3&rft_id=info%3Apmid%2F20617390&rft.externalDocID=PMC4770897 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1536-1632&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1536-1632&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1536-1632&client=summon |