Use of macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration of healthy dermis in humans: An in vivo study
If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are availab...
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| Published in | Journal of plastic, reconstructive & aesthetic surgery Vol. 63; no. 5; pp. 848 - 857 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Kidlington
Elsevier Ltd
01.05.2010
Elsevier |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1748-6815 1878-0539 1878-0539 |
| DOI | 10.1016/j.bjps.2009.01.068 |
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| Abstract | If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration.
In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane
®). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed.
The Restylane
® merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time.
The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject.
Further
in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. |
|---|---|
| AbstractList | Summary If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane® ). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed. The Restylane® merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed. The Restylane merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration.If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed. The Restylane merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane ®). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed. The Restylane ® merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno)histological results were analysed. The Restylane merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the surroundings that migrate into them and start to produce autologous extracellular matrix as the scaffold is degraded. Several materials are available, but most of them are in the form of sheets and need to be laid on an open wound surface. A number of injectable fillers have been developed to correct soft-tissue defects. However, none of these has been used for guided tissue regeneration. We present a new technique that could possibly be used to correct dermal defects by using macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration. In eight healthy volunteers, intradermal injections of macroporous gelatine spheres were compared with injections of saline and hyaluronic acid (Restylane (R)). Full-thickness skin biopsy specimens of the implants and surrounding tissue were removed 2, 8, 12 and 26 weeks after injection, and the (immuno) histological results were analysed. The Restylane (R) merely occupied space. It shattered the dermal tissue and compressed collagen fibres and cells at the interface between the implant and the dermis. No regeneration of tissue was found with this material at any time. The macroporous gelatine spheres were populated with fibroblasts already after 2 weeks. After 8 weeks the spheres were completely populated by fibroblasts producing dermal tissue. After 12 and 26 weeks, the gelatine spheres had been more or less completely resorbed and replaced by vascularised neodermis. There were no signs of capsular formation, rejection or adverse events in any subject. Further in vivo studies in humans are needed to evaluate the effect of the macroporous spheres fully as a matrix for guided tissue regeneration with and without cellular pre-seeding. However, the results of this study indicate the possibility of using macroporous gelatine spheres as an injectable, three-dimensional, degradable matrix for guided tissue regeneration. |
| Author | Huss, Fredrik R.M. Kratz, Gunnar Nyman, Erika Bolin, Johanna S.C. |
| Author_xml | – sequence: 1 givenname: Fredrik R.M. surname: Huss fullname: Huss, Fredrik R.M. email: fredrik.huss@lio.se organization: Department of Hand-, Plastic-, and Burn Surgery, University Hospital of Linköping, Sweden – sequence: 2 givenname: Erika surname: Nyman fullname: Nyman, Erika organization: Department of Hand-, Plastic-, and Burn Surgery, University Hospital of Linköping, Sweden – sequence: 3 givenname: Johanna S.C. surname: Bolin fullname: Bolin, Johanna S.C. organization: Laboratory for Experimental Plastic Surgery, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköpings universitet, Linköping, Sweden – sequence: 4 givenname: Gunnar surname: Kratz fullname: Kratz, Gunnar organization: Department of Hand-, Plastic-, and Burn Surgery, University Hospital of Linköping, Sweden |
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| CitedBy_id | crossref_primary_10_1371_journal_pone_0128093 crossref_primary_10_3390_ma2041908 crossref_primary_10_1021_acs_langmuir_1c01508 crossref_primary_10_1016_j_fsc_2015_07_002 crossref_primary_10_1177_1558944715628005 crossref_primary_10_1371_journal_pone_0221878 crossref_primary_10_1089_ten_tea_2013_0207 crossref_primary_10_1371_journal_pone_0109214 crossref_primary_10_1016_j_msec_2014_10_051 crossref_primary_10_1097_PRS_0000000000000106 crossref_primary_10_3892_etm_2017_5020 crossref_primary_10_1016_j_burns_2010_03_014 crossref_primary_10_1016_j_biomaterials_2011_04_015 crossref_primary_10_1039_c0sm01302a crossref_primary_10_1002_biot_201700087 crossref_primary_10_1016_j_addr_2012_04_008 crossref_primary_10_1016_j_bej_2013_11_014 crossref_primary_10_3390_magnetochemistry10040020 crossref_primary_10_1002_btpr_618 |
| Cites_doi | 10.1038/nbt1186-989 10.1001/archfaci.1.3.165 10.1126/science.8493529 10.1007/s00266-003-3022-1 10.1016/S0142-9612(03)00428-9 10.1016/S0142-9612(03)00374-0 10.1016/j.biomaterials.2003.10.072 10.1007/s00266-002-2048-0 10.1097/00007890-199507150-00001 10.1111/j.1067-1927.2004.012205.x 10.1097/00006534-200001000-00057 10.1126/science.7031899 10.1016/j.bjps.2005.10.031 10.1001/archderm.143.2.155 |
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| Copyright | 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons British Association of Plastic, Reconstructive and Aesthetic Surgeons 2015 INIST-CNRS Copyright (c) 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved. |
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| Keywords | Soft-tissue defect Human Plastic surgery Tissue engineering Filler Guided tissue regeneration Soft tissue defect Absorbable Guided tissue Guidance In vivo Regeneration Treatment Derm |
| Language | English |
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| References | Watson, Keller, Lacombe (bib8) 1999; 1 Huss, Junker, Johnson (bib12) 2007; 60 Yannas (bib3) 1988 Langer, Vacanti (bib1) 1993; 260 Liu, Hafner, Dragieva (bib13) 2004; 12 Yurugi, Hatoko, Kuwahara (bib6) 2002; 26 Park, Kim, Suh (bib7) 2004; 25 Nilsson, Buzsaky, Mosbach (bib11) 1986; 4 Wang, Garza, Kang (bib15) 2007 Feb; 143 Ma, Gao, Mao (bib5) 2003; 24 Yannas, Burke, Orgill (bib4) 1982; 215 Fagien (bib9) 2000; 105 Livesey, Herndon, Hollyoak (bib2) 1995; 60 Malda, Kreijveld, Temenoff (bib14) 2003; 24 Lemperle, Morhenn, Charrier (bib10) 2003; 27 Lemperle (10.1016/j.bjps.2009.01.068_bib10) 2003; 27 Liu (10.1016/j.bjps.2009.01.068_bib13) 2004; 12 Yurugi (10.1016/j.bjps.2009.01.068_bib6) 2002; 26 Fagien (10.1016/j.bjps.2009.01.068_bib9) 2000; 105 Malda (10.1016/j.bjps.2009.01.068_bib14) 2003; 24 Yannas (10.1016/j.bjps.2009.01.068_bib3) 1988 Wang (10.1016/j.bjps.2009.01.068_bib15) 2007; 143 Park (10.1016/j.bjps.2009.01.068_bib7) 2004; 25 Yannas (10.1016/j.bjps.2009.01.068_bib4) 1982; 215 Langer (10.1016/j.bjps.2009.01.068_bib1) 1993; 260 Huss (10.1016/j.bjps.2009.01.068_bib12) 2007; 60 Ma (10.1016/j.bjps.2009.01.068_bib5) 2003; 24 Nilsson (10.1016/j.bjps.2009.01.068_bib11) 1986; 4 Watson (10.1016/j.bjps.2009.01.068_bib8) 1999; 1 Livesey (10.1016/j.bjps.2009.01.068_bib2) 1995; 60 |
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| Snippet | If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from the... Summary If a biodegradable scaffold is applied, the dermis can be regenerated by guided tissue regeneration. Scaffolds can stimulate in-growth of cells from... |
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| SubjectTerms | Absorbable Implants Adult Biological and medical sciences Biopsy Biotechnology Dermis - cytology Dermis - physiology Fibroblasts - cytology Fibroblasts - drug effects Filler Fundamental and applied biological sciences. Psychology Gelatin - administration & dosage Guided tissue regeneration Health. Pharmaceutical industry Human Humans Hyaluronic Acid - administration & dosage Hyaluronic Acid - analogs & derivatives Industrial applications and implications. Economical aspects Injections, Intradermal Medical sciences MEDICIN MEDICINE Miscellaneous Plastic Surgery Porosity Reconstructive Surgical Procedures - methods Reference Values Regeneration - physiology Soft Tissue Injuries - surgery Soft-tissue defect Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue engineering Tissue Scaffolds Wound Healing - drug effects Young Adult |
| Title | Use of macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration of healthy dermis in humans: An in vivo study |
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