Noninvasive method for assessing the human circadian clock using hair follicle cells
A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhyth...
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| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 35; pp. 15643 - 15648 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences
31.08.2010
National Acad Sciences |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0027-8424 1091-6490 1091-6490 |
| DOI | 10.1073/pnas.1003878107 |
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| Abstract | A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting. |
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| AbstractList | A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting. A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting. [PUBLICATION ABSTRACT] A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting.A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting. |
| Author | Nishida, Eisuke Yasuda, Akio Akashi, Makoto Yamamoto, Takuya Takahashi, Joseph S. Yamamoto, Takuro Tsugitomi, Asuka Yamashita, Shiko Node, Koichi Liao, James K. Soma, Haruhiko |
| Author_xml | – sequence: 1 givenname: Makoto surname: Akashi fullname: Akashi, Makoto – sequence: 2 givenname: Haruhiko surname: Soma fullname: Soma, Haruhiko – sequence: 3 givenname: Takuro surname: Yamamoto fullname: Yamamoto, Takuro – sequence: 4 givenname: Asuka surname: Tsugitomi fullname: Tsugitomi, Asuka – sequence: 5 givenname: Shiko surname: Yamashita fullname: Yamashita, Shiko – sequence: 6 givenname: Takuya surname: Yamamoto fullname: Yamamoto, Takuya – sequence: 7 givenname: Eisuke surname: Nishida fullname: Nishida, Eisuke – sequence: 8 givenname: Akio surname: Yasuda fullname: Yasuda, Akio – sequence: 9 givenname: James K. surname: Liao fullname: Liao, James K. – sequence: 10 givenname: Koichi surname: Node fullname: Node, Koichi – sequence: 11 givenname: Joseph S. surname: Takahashi fullname: Takahashi, Joseph S. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20798039$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Algorithms Animals Biological Clocks - physiology Biological Sciences Biopsy Cells Circadian rhythm Circadian Rhythm - physiology Circadian rhythms CLOCK Proteins - genetics Cosine function Female Gene expression Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Hair Hair cells Hair Follicle - cytology Hair Follicle - metabolism Hair follicles head Humans Instructional materials Lifestyle Male Mice Models, Genetic Nuclear Receptor Subfamily 1, Group D, Member 1 - genetics Oligonucleotide Array Sequence Analysis Period Circadian Proteins - genetics Receptors, Cytoplasmic and Nuclear - genetics Repressor Proteins - genetics Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA Rotating shifts Scalp |
| Title | Noninvasive method for assessing the human circadian clock using hair follicle cells |
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