Cdc28 Activates Exit from Mitosis in Budding Yeast

• Published in: The Journal of cell biology Vol. 149; no. 7; pp. 1361 - 1376
• Main Authors: Rudner, Adam D., Hardwick, Kevin G., Murray, Andrew W.
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 26.06.2000; The Rockefeller University Press
• Subjects: anaphase; Anaphase - physiology; anaphase-promoting complex; binding proteins; CDC2-CDC28 Kinases; Cdc20 Proteins; CDC28 gene; Cdh1 Proteins; Cell Cycle Proteins; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell division; Cellular biology; cyclin-dependent kinase; cytology; enzyme activity; Fungal Proteins; Fungal Proteins - genetics; Fungal Proteins - metabolism; G1 Phase; G1 Phase - physiology; Genes, cdc; Genes, cdc - physiology; genetics; interphase; metabolism; mitosis; Mitosis - physiology; mutants; Mutation; Mutation - genetics; Original; phenotype; Phosphorylation; physiology; Protein Serine-Threonine Kinases; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Saccharomycetales; Saccharomycetales - cytology; Saccharomycetales - genetics; Saccharomycetales - metabolism; Spindle Apparatus; Spindle Apparatus - genetics; Spindle Apparatus - metabolism; Time Factors; Yeast; Yeasts
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.149.7.1361

The activity of the cyclin-dependent kinase 1 (Cdk1), Cdc28, inhibits the transition from anaphase to G1 in budding yeast. CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive to perturbations that arrest cells in mitosis. Surprisingly, this behavior is not due to a lack of inhibitory phosphorylation or increased kinase activity, but reflects reduced activity of the anaphase-promoting complex (APC), a defect shared with other mutants that lower Cdc28/Clb activity in mitosis. CDC28-VF has reduced Cdc20-dependent APC activity in mitosis, but normal Hct1-dependent APC activity in the G1 phase of the cell cycle. The defect in Cdc20-dependent APC activity in CDC28-VF correlates with reduced association of Cdc20 with the APC. The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast.