多壁碳纳米管修饰铂基片石英晶体微天平研究白细胞介素-6与其受体的相互作用

在pH 7.4、室温条件下,用多壁碳纳米管（MWCNTs）修饰石英晶体微天平（QCM）Pt基片,经过EDC/NHS活化后固定白细胞介素-6（IL-6）,检测不同浓度可溶性白细胞介素-6受体（sIL-6R）与IL-6的结合所引起响应信号的变化,并用Origin软件对数据进行拟合分析,构建了一种高性能实时监测IL-6与sIL-6R之间相互作用的亲和型生物传感器。实验表明：随着sIL-6R浓度的增加,其结合量呈线性增长,相关系数（R2）为0.997。IL-6与sIL-6R之间相互作用的结合平衡常数（KA）和解离平衡常数（KD）分别为3.37×106 L/mol和2.97×10-7 mol/L。验证了...

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Bibliographic Details
Published in分析化学 Vol. 39; no. 9; pp. 1302 - 1306
Main Author 房钰鑫 商洪才 秦霞 樊官伟 张迪 赵薇 李争春 张伯礼 陈强
Format Journal Article
LanguageChinese
Published 南开大学生命科学学院,生物活性材料教育部重点实验室,天津300071%天津中医药大学中医药研究院,天津,300193 2011
Subjects
Pt基片
可溶性白细胞介素-6受体
多壁碳纳米管
白细胞介素-6
石英晶体微天平
Pt基片
白细胞介素-6
石英晶体微天平
可溶性白细胞介素-6受体
多壁碳纳米管
Online AccessGet full text
ISSN0253-3820
DOI10.3724/SP.J.1096.2011.01302

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Summary:在pH 7.4、室温条件下,用多壁碳纳米管（MWCNTs）修饰石英晶体微天平（QCM）Pt基片,经过EDC/NHS活化后固定白细胞介素-6（IL-6）,检测不同浓度可溶性白细胞介素-6受体（sIL-6R）与IL-6的结合所引起响应信号的变化,并用Origin软件对数据进行拟合分析,构建了一种高性能实时监测IL-6与sIL-6R之间相互作用的亲和型生物传感器。实验表明：随着sIL-6R浓度的增加,其结合量呈线性增长,相关系数（R2）为0.997。IL-6与sIL-6R之间相互作用的结合平衡常数（KA）和解离平衡常数（KD）分别为3.37×106 L/mol和2.97×10-7 mol/L。验证了通过MWCNTs修饰的Pt QCM能够高效监测IL-6与sIL-6R间相互作用及动力学过程,有助于阐明二者的生物学功能。
Bibliography:Multi-walled carbon nanotubes; Platinum electrode; Quartz crystal microbalance; Interleukin-6; Soluble interleukin-6 receptor
22-1125/O6
An affinity biosensor using multi-walled carbon nanotubes to activate Pt quartz crystal microbalance（QCM） electrode and immobilizing interleukin-6（IL-6） on 1-ethyl-3-（3-dimethylaminopropyl） carbodimide（EDC）/N-hydroxysuccinimide（NHS）-activated QCM electrode was employed to real time detect the interaction and kinetic analysis of IL-6 and soluble interleukin-6 receptor（sIL-6R）,and the association data between IL-6 and sIL-6R was fitted by Origin software.The results showed that the combined masses of sIL-6R on IL-6-EDC/NHS-MWCNTs activated Pt QCM increased linearly with the increase of concentration of sIL-6R,and the linear correlation coefficient was 0.997.For the interaction,KA was 3.37×106 L/mol,and KD was 2.97×10-7 mol/L.The experiment proves the feasibility of using multi-walled carbon nanotubes activated Pt quartz crystal microbalance to monitor the interaction and kinetic
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2011.01302