离子排斥色谱法测定生脉注射液中的有机酸

1引言 生脉注射液是由红参、麦冬和五味子3种药材经提取后制成的灭菌水溶液,为国家中药保护品种,具有益气养阴,复脉固脱的功效。前期的分析实验表明,生脉注射液中含有有机酸类成分。根据生脉注射液的生产工艺和3种药材的的化学成分研究报道,分析生脉注射液中的有机酸主要来源于五味子。五味子含有柠檬酸、苹果酸、琥珀酸等多种有机酸成分。目前,有机酸的分析方法有气相色谱法[1]、高效液相色谱法[2]、毛细管电泳法[3]及离子色谱法[4]。...

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Bibliographic Details
Published in分析化学 Vol. 40; no. 3; pp. 489 - 490
Main Author 刘瑞 聂黎行 孙焕 李涛 王钢力 林瑞超
Format Journal Article
LanguageChinese
Published 中国食品药品检定研究院,北京100050%北京中医药大学中药学院,北京,100029%瑞士万通中国有限公司中心实验室,北京,100005%中国食品药品检定研究院,北京,100050 2012
北京中医药大学中药学院,北京100029
Subjects
化学成分
有机酸类
毛细管电泳法
气相色谱法
测定
生脉注射液
离子排斥色谱法
高效液相色谱法
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ISSN0253-3820
DOI10.3724/SP.J.1096.2012.10799

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Summary:1引言 生脉注射液是由红参、麦冬和五味子3种药材经提取后制成的灭菌水溶液,为国家中药保护品种,具有益气养阴,复脉固脱的功效。前期的分析实验表明,生脉注射液中含有有机酸类成分。根据生脉注射液的生产工艺和3种药材的的化学成分研究报道,分析生脉注射液中的有机酸主要来源于五味子。五味子含有柠檬酸、苹果酸、琥珀酸等多种有机酸成分。目前,有机酸的分析方法有气相色谱法[1]、高效液相色谱法[2]、毛细管电泳法[3]及离子色谱法[4]。
Bibliography:LIU Rui,NIE Li-Xing ,SUN Huan,LI Tao,WANG Gang-Li,LIN Rui-Chao1（Beijing University of Chinese Medicine,School of Chinese Materia Medica,Beijing 100029,China）2（National Institute for Food and Drug Control,Beijing 100050,China）3（Metrohm China Limited,Central Laboratory,Beijing 100005,China）
A method was developed for the separation and determination of three organic acid,viz citric acid,malic acid and succinic acid in Sheng-mai Injection by ion exclusion chromatography with suppressed conductivity detection.The chromatographic separation was performed on a Transgenomic Sep-ION-300 column（300 mm length ×7.8 mm i.d.,8 μm） at 70 ℃ and eluted with a mixture of 5 mmol/L sulphuric acid and acetone（95 ∶ 5） at the flow rate of 0.4 mL/min.The effects of the concentration of sulphuric acid,the volume ratio of acetone and column temperature were investigated and optimized.The calibration curve for citric acid,malic acid and succinic acid was in a good linearity in the range of 7.45-476.5 mg/L,14-521.0 mg/L and 3.47-222.0 m
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2012.10799