Identification of Potential Key lncRNAs and Genes Associated with Aging Based on Microarray Data of Adipocytes from Mice

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 11
• Main Authors: Yu, Weigang, Meng, Songyan, Teng, Zongyan, Yang, Yi
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc
• Subjects: Adipocytes; Adipocytes - metabolism; Adipocytes - pathology; Adipose tissues; Age; Aging; Aging - genetics; Aging - pathology; Algorithms; Animals; Antisense RNA; Biomedical research; Biosynthesis; Bone marrow; Cholesterol; Cluster Analysis; Gene expression; Gene Expression Profiling; Gene Regulatory Networks - genetics; Genetic aspects; Health aspects; Humans; Metabolism; Methods; Mice; Oligonucleotide Array Sequence Analysis; Protein synthesis; Proteins; RNA sequencing; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; RNA, Long Noncoding - isolation & purification; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Senescence; Studies; Time series; Transcriptome - genetics
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2016/9181702

Objective. This study aimed to screen potential crucial lncRNAs and genes involved in aging. Methods. The data of 9 peripheral white adipocytes, respectively, taken from male C57BL/6J mice (6 months, 14 months, and 18 months of age) in GSE25905 were used in this study. Differentially time series expressed lncRNA genes (DE-lncRNAs) and mRNA genes (DEGs) were identified. After cluster analysis of lncRNAs expression pattern, target genes of DE-lncRNAs were predicted from the DEGs, and functional analysis for target genes was conducted. Results. A total of 8301 time series-related DEGs and 43 time series-related DE-lncRNAs were identified. Among them, 41 DE-lncRNAs targeted 1880 DEGs. The DEGs positively regulated by DE-lncRNAs were mainly related to the development of blood vessel and the pathways of cholesterol biosynthesis and elastic fibre formation. Furthermore, the DEGs negatively regulated by DE-lncRNAs were correlated with protein metabolism. Conclusion. These DE-lncRNAs and DEGs are potentially involved in the process of aging.