高效液相色谱法测定~（18）F-FDG注射液中氨基聚醚含量
采用高效液相色谱法测定了18F-FDG注射液中氨基聚醚（K2.2.2）的含量,并对流动相、流速、检测波长进行了选择,确定检测条件为：V（50mmol/L乙酸铵）∶V（乙腈）=1∶1为流动相,流速为0.5mL/min,检测波长210nm,进样量为10μL。K2.2.2在1～50mg/L浓度范围内线性良好,线性回归方程：y=265 939.4x＋7 490.7,相关系数γ=0.999,回收率为96.0%～100.3%,精密度小于3.5%。本方法回收率较高,精密度较小,样品用量少,可用于18F-FDG注射液中微量K2.2.2的测定。...
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| Published in | 同位素 Vol. 25; no. 3; pp. 171 - 174 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
原子高科股份有限公司,北京,102413
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-7512 |
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| Summary: | 采用高效液相色谱法测定了18F-FDG注射液中氨基聚醚（K2.2.2）的含量,并对流动相、流速、检测波长进行了选择,确定检测条件为：V（50mmol/L乙酸铵）∶V（乙腈）=1∶1为流动相,流速为0.5mL/min,检测波长210nm,进样量为10μL。K2.2.2在1～50mg/L浓度范围内线性良好,线性回归方程：y=265 939.4x＋7 490.7,相关系数γ=0.999,回收率为96.0%～100.3%,精密度小于3.5%。本方法回收率较高,精密度较小,样品用量少,可用于18F-FDG注射液中微量K2.2.2的测定。 |
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| Bibliography: | 11-2566/TL 18 F-FDG injection; Kryptofix2.2.2; high performance liquid chromatography A high-performance liquid chromatograph method was used to quantify Krypto- fix2.2.2 （K2.2.2）in the radiopharmaceutical of 18F-FDG（2-Deoxy-2-[18F] fluoro-D-glucose）. A Waters C18 column （ XTerra RP18 150 mm×3. 9 mm,5 μm）was used as stationary phase, V（50 mmol/L ammonium acetate） ： V（acetonitrile）=1 ： 1 was used as mobile phase and the flow was 0.5 mL · min^-1, UV detection wavelength was performed at 210 nm. The calibration curve was established over the rang of 1-50 mg/L, the linear correlation co- efficient was 0. 999, and the recovery was in the rang of 96.0%-100.3%. The method was accurate and using less sample,and can be used for the quality control of K2.2.2 in 18F-FDG injection production. ZHAO Yan, WANG Xiao-jing, YE Zhao-yun, ZHAO Xiu-yan, JIANG Hua （HTA Co. , LTD. , Beijing 102413, China） |
| ISSN: | 1000-7512 |