Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution

• Published in: Cell Vol. 176; no. 4; pp. 743 - 756.e17
• Main Authors: Pollen, Alex A., Bhaduri, Aparna, Andrews, Madeline G., Nowakowski, Tomasz J., Meyerson, Olivia S., Mostajo-Radji, Mohammed A., Di Lullo, Elizabeth, Alvarado, Beatriz, Bedolli, Melanie, Dougherty, Max L., Fiddes, Ian T., Kronenberg, Zev N., Shuga, Joe, Leyrat, Anne A., West, Jay A., Bershteyn, Marina, Lowe, Craig B., Pavlovic, Bryan J., Salama, Sofie R., Haussler, David, Eichler, Evan E., Kriegstein, Arnold R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.02.2019
• Subjects: Animals; Biological Evolution; brain; Brain - cytology; Cell Culture Techniques - methods; Cell Differentiation - genetics; Cerebral Cortex - cytology; Cerebral Cortex - metabolism; cerebral organoids; chimpanzee; cortex; cortical development; evolution; gene duplication; gene regulatory networks; Gene Regulatory Networks - genetics; genes; human-specific evolution; Humans; Induced Pluripotent Stem Cells - cytology; Macaca; macaque; mTOR; neural progenitor cells; neurogenesis; Neurogenesis - genetics; Organoids - growth & development; Organoids - metabolism; Pan troglodytes; Pluripotent Stem Cells - cytology; radial glia; receptors; Single-Cell Analysis; single-cell RNA sequencing; Species Specificity; Transcriptome - genetics; single-cell RNA sequencing; cerebral organoids; macaque; radial glia; human-specific evolution; mTOR; chimpanzee; cortical development; neural progenitor cells
• ISSN: 0092-8674; 1097-4172; 1097-4172
• DOI: 10.1016/j.cell.2019.01.017

Direct comparisons of human and non-human primate brains can reveal molecular pathways underlying remarkable specializations of the human brain. However, chimpanzee tissue is inaccessible during neocortical neurogenesis when differences in brain size first appear. To identify human-specific features of cortical development, we leveraged recent innovations that permit generating pluripotent stem cell-derived cerebral organoids from chimpanzee. Despite metabolic differences, organoid models preserve gene regulatory networks related to primary cell types and developmental processes. We further identified 261 differentially expressed genes in human compared to both chimpanzee organoids and macaque cortex, enriched for recent gene duplications, and including multiple regulators of PI3K-AKT-mTOR signaling. We observed increased activation of this pathway in human radial glia, dependent on two receptors upregulated specifically in human: INSR and ITGB8. Our findings establish a platform for systematic analysis of molecular changes contributing to human brain development and evolution. •Brain organoids preserve gene expression networks despite elevated metabolic stress•Chimpanzee organoids enable studies of the evolution of human brain development•Primary and organoid samples reveal 261 human-specific gene expression changes•Human radial glia exhibit increased mTOR activation compared to non-human primates Comparisons of cerebral organoids between chimpanzees, macaques, and humans reveal gene duplications and cell-signaling alterations that explain developmental evolutionary differences that are unique to us as a species.