Molecular karyotype of the white rot fungus Pleurotus ostreatus

The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic con...

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Published in	Applied and environmental microbiology Vol. 65; no. 8; pp. 3413 - 3417
Main Authors	Larraya, L.M, Perez, G, Penas, M.M, Baars, J.J.P, Mikosch, T.S.P, Pisabarro, A.G, Ramirez, L
Format	Journal Article
Language	English
Published	Washington, DC American Society for Microbiology 01.08.1999
Subjects	Base Sequence Biological and medical sciences Biotechnology breeding chromosome number chromosome segregation chromosomes Chromosomes, Fungal Chromosomes, Fungal - genetics Cytogenetics DNA probes DNA Probes - genetics Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Fungi genetic engineering genetics Genetics of eukaryotes. Biological and molecular evolution Genomics Karyotypes Karyotyping Molecular Sequence Data Molecules Mycology Pleurotus Pleurotus - genetics Pleurotus ostreatus pulsed field electrophoresis pulsed-field gel electrophoresis Thallophyta, bryophyta Vegetals and fungi white-rot fungi Fungi Pleurotus ostreatus Cytogenetics Basidiomycetes Chromosome Karyotype Edible fungi Thallophyta
Online Access	Get full text
ISSN	0099-2240 1070-6291 1098-5336 1098-5336
DOI	10.1128/AEM.65.8.3413-3417.1999

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Abstract	The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species.
AbstractList	The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species. The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species. The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species.The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species. The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure.
Author	Mikosch, T.S.P Perez, G Pisabarro, A.G Penas, M.M Larraya, L.M Ramirez, L Baars, J.J.P
AuthorAffiliation	Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain, 1 and Mushroom Experimental Station, NL-5960 AA Horst, The Netherlands 2
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Keywords	Fungi Pleurotus ostreatus Cytogenetics Basidiomycetes Chromosome Karyotype Edible fungi Thallophyta
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Notes	SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-2 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain. Phone: (34) 948 169 130. Fax: (34) 948 169 732. E-mail: lramirez@upna.es.
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Snippet	The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and... The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and...
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SubjectTerms	Base Sequence Biological and medical sciences Biotechnology breeding chromosome number chromosome segregation chromosomes Chromosomes, Fungal Chromosomes, Fungal - genetics Cytogenetics DNA probes DNA Probes - genetics Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Fungi genetic engineering genetics Genetics of eukaryotes. Biological and molecular evolution Genomics Karyotypes Karyotyping Molecular Sequence Data Molecules Mycology Pleurotus Pleurotus - genetics Pleurotus ostreatus pulsed field electrophoresis pulsed-field gel electrophoresis Thallophyta, bryophyta Vegetals and fungi white-rot fungi
Title	Molecular karyotype of the white rot fungus Pleurotus ostreatus
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