Characterization in vitro and in vivo of the putative multigene 4-coumarate:CoA ligase network in Arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation - Costa, Michael A.; Bedgar, Diana L.; Moinuddin, Syed G.A.; Kim, Kye-Won; Cardenas, Claudia L.; Cochrane, Fiona C.; Shockey, Jay M.; Helms, Gregory L.; Amakura, Yoshiaki; Takahashi, Hironobu; Milhollan, Jessica K.; Davin, Laurence B.; et al. (Phytochemistry (Oxford), 2005)
Keywords: 4-Coumarate CoA ligase homologues; 4-Coumarate CoA ligases; Alcohols; Alcohols - chemistry; Alcohols - metabolism; amino acid sequences; Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis thaliana; Base Sequence; Biological and medical sciences; biosynthesis; Chemical constitution; chemical synthesis; chemistry; chromosomes; Coenzyme A Ligases; Coenzyme A Ligases - genetics; Coenzyme A Ligases - metabolism; coumarate-CoA ligase; Coumaric Acids; Coumaric Acids - chemistry; Cruciferae; DNA Primers; enzymology; Fundamental and applied biological sciences. Psychology; Genes, Plant; genetics; genome; Knockout analysis; Lignin; Lignin - chemical synthesis; Lignin - metabolism; Metabolic networks; Metabolism; Metabolism. Physicochemical requirements; Molecular Sequence Data; multigene family; nucleotide sequences; plant biochemistry; Plant physiology and development; plant proteins; recombinant fusion proteins; Sinapate/sinapyl alcohol derivatives; sinapic acid; sinapyl alcohol; syringyl lignin; Lignin; 4-Coumarate CoA ligase homologues; G; TIGR; Metabolic networks; H; Knockout analysis; Sinapate/sinapyl alcohol derivatives; Arabidopsis thaliana; TAIR; S; Cruciferae; RT-PCR; IPTG; 4-Coumarate CoA ligases; 4CL; WT; CWR; Cinnamic acid; Alcohol; Phylogeny; Stem; EC 6.2.1.12; Characterization; Ester; Gene; Dicotyledones; Substrate specificity; Angiospermae; Recombinant protein; Enzyme; In vitro; Caffeic acid; Screening; In vivo; DNA; Spermatophyta; Kinetics