局部冷冻对G422胶质瘤细胞凋亡的影响

R730.56%R739.41; 目的:观察局部冷冻对胶质瘤细胞凋亡的影响,以及不同冻融周期与细胞凋亡之间的关系,进一步探讨冷冻杀伤肿瘤细胞的分子机制.方法:建立胶质瘤动物模型,应用末端标记技术原位检测细胞凋亡,DNA琼脂糖电泳观察DNA梯形条带.结果:经不同冻融周期冷冻G422胶质瘤细胞后,在不同时间点,冷冻周围区的肿瘤细胞出现了形态学和DNA水平上的细胞凋亡变化,其峰值出现在冷冻后24～48 h,重复冻融后出现更多的凋亡细胞.结论:冷冻治疗可以诱导G422胶质瘤细胞发生凋亡,细胞凋亡的数目与冻融周期有关.诱导肿瘤细胞凋亡可能是冷冻杀伤肿瘤细胞的重要机制....

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Published in癌症 Vol. 19; no. 10; pp. 865 - 867
Main Authors 秦军, 涂汉军, 席刚明, 王伦长
Format Journal Article
LanguageChinese
Published 湖北省十堰市太和医院神经外科,湖北,十堰,442000 2000
Subjects
细胞凋亡
冷冻治疗
胶质瘤
Online AccessGet full text
ISSN1000-467X
DOI10.3321/j.issn:1000-467X.2000.10.007

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Abstract R730.56%R739.41; 目的:观察局部冷冻对胶质瘤细胞凋亡的影响,以及不同冻融周期与细胞凋亡之间的关系,进一步探讨冷冻杀伤肿瘤细胞的分子机制.方法:建立胶质瘤动物模型,应用末端标记技术原位检测细胞凋亡,DNA琼脂糖电泳观察DNA梯形条带.结果:经不同冻融周期冷冻G422胶质瘤细胞后,在不同时间点,冷冻周围区的肿瘤细胞出现了形态学和DNA水平上的细胞凋亡变化,其峰值出现在冷冻后24～48 h,重复冻融后出现更多的凋亡细胞.结论:冷冻治疗可以诱导G422胶质瘤细胞发生凋亡,细胞凋亡的数目与冻融周期有关.诱导肿瘤细胞凋亡可能是冷冻杀伤肿瘤细胞的重要机制.
AbstractList R730.56%R739.41; 目的:观察局部冷冻对胶质瘤细胞凋亡的影响,以及不同冻融周期与细胞凋亡之间的关系,进一步探讨冷冻杀伤肿瘤细胞的分子机制.方法:建立胶质瘤动物模型,应用末端标记技术原位检测细胞凋亡,DNA琼脂糖电泳观察DNA梯形条带.结果:经不同冻融周期冷冻G422胶质瘤细胞后,在不同时间点,冷冻周围区的肿瘤细胞出现了形态学和DNA水平上的细胞凋亡变化,其峰值出现在冷冻后24～48 h,重复冻融后出现更多的凋亡细胞.结论:冷冻治疗可以诱导G422胶质瘤细胞发生凋亡,细胞凋亡的数目与冻融周期有关.诱导肿瘤细胞凋亡可能是冷冻杀伤肿瘤细胞的重要机制.
Author 秦军
席刚明
涂汉军
王伦长
AuthorAffiliation 湖北省十堰市太和医院神经外科,湖北,十堰,442000
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冷冻治疗
胶质瘤
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Title 局部冷冻对G422胶质瘤细胞凋亡的影响
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