Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma

• Published in: International journal of oncology Vol. 44; no. 2; pp. 600 - 608
• Main Authors: PRZYBYLA, BEATA D, SHAFIRSTEIN, GAL, VISHAL, SAGAR J, DENNIS, RICHARD A, GRIFFIN, ROBERT J
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.02.2014; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: 4T1 tumors; Ablation (Surgery); Animals; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Blotting, Western; Bone marrow; Bone Marrow Neoplasms - genetics; Bone Marrow Neoplasms - metabolism; Bone Marrow Neoplasms - secondary; Breast cancer; Care and treatment; Chemokines - blood; Cxcl12; Cytokines; Cytokines - blood; Disease Models, Animal; Esele; Female; Fgf2; Gene expression; Gene Expression Profiling; Genetic aspects; Health aspects; Heat; Heat shock proteins; HSP27; HSP70; Humans; Hyperthermia, Induced; Hypoxia; Lifr; Mammary Neoplasms, Experimental - genetics; Mammary Neoplasms, Experimental - metabolism; Mammary Neoplasms, Experimental - pathology; Metastasis; Mice; Mice, Inbred BALB C; Mice, Nude; Oligonucleotide Array Sequence Analysis; Physiological aspects; Proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Rodents; SDF1; Stress response; Studies; Temperature; thermal ablation; Tumor Cells, Cultured; Tumors; Ultrasonic imaging; Arkansas
• ISSN: 1019-6439; 1791-2423
• DOI: 10.3892/ijo.2013.2185

Thermal ablation of solid tumors using conductive interstitial thermal therapy (CITT) produces coagulative necrosis in the center of ablation. Local changes in homeostasis for surviving tumor and systemic changes in circulation and distant organs must be understood and monitored in order to prevent tumor re-growth and metastasis. The purpose of this study was to use a mouse carcinoma model to evaluate molecular changes in the bone marrow and surviving tumor after CITT treatment by quantification of transcripts associated with cancer progression and hyperthermia, serum cytokines, stress proteins and the marrow/tumor cross-talk regulator stromal-derived factor 1. Analysis of 27 genes and 22 proteins with quantitative PCR, ELISA, immunoblotting and multiplex antibody assays revealed that the gene and protein expression in tissue and serum was significantly different between ablated and control mice. The transcripts of four genes (Cxcl12, Sele, Fgf2, Lifr) were significantly higher in the bone marrow of treated mice. Tumors surviving ablation showed significantly lower levels of the Lifr and Sele transcripts. Similarly, the majority of transcripts measured in tumors decreased with treatment. Surviving tumors also contained lower levels of SDF-1α and HIF-1α proteins whereas HSP27 and HSP70 were higher. Of 16 serum chemokines, IFNγ and GM-CSF levels were lower with treatment. These results indicate that CITT ablation causes molecular changes which may slow cancer cell proliferation. However, inhibition of HSP27 may be necessary to control aggressiveness of surviving cancer stem cells. The changes in bone marrow are suggestive of possible increased recruitment of circulatory cancer cells. Therefore, the possibility of heightened bone metastasis after thermal ablation needs to be further investigated and inhibition strategies developed, if warranted.