PACAP activates MRGPRX2 on meningeal mast cells to drive migraine-like pain

• Published in: Scientific reports Vol. 13; no. 1; pp. 12302 - 9
• Main Authors: Sbei, Sami, Moncrief, Taylor, Limjunyawong, Nathachit, Zeng, Yaping, Green, Dustin P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.07.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/250/2504; 631/250/371; 631/378/2620/410; Animals; Cell activation; Cells; Connective tissue; Connective tissues; G protein-coupled receptors; Headache; Headaches; Humanities and Social Sciences; Humans; Mast Cells; Meninges; Mice; Mice, Transgenic; Migraine; Migraine Disorders - chemically induced; multidisciplinary; Nerve Tissue Proteins - genetics; Neuropeptides; Neurosciences; Pain; Pathophysiology; Peptides; Pituitary adenylate cyclase-activating polypeptide; Pituitary Adenylate Cyclase-Activating Polypeptide - genetics; Quality of Life; Receptors, G-Protein-Coupled - genetics; Receptors, Neuropeptide - genetics; Science; Science (multidisciplinary); Transgenic mice
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-39571-y

Migraine ranks among the most prevalent disorders worldwide, leading to disability and decreased quality of life in patients. Recently, neurogenic inflammation has been recognized as a potential underlying pathology contributing to the migraine pain pathway. Mast cells reside in the meninges and have been implicated in contributing to the pathophysiology of migraine. Here we report for the first time that the mouse Mas-Related G-protein-coupled Receptor B2 (MrgprB2), is expressed on meningeal connective tissue mast cells and contributes to Pituitary Adenylate Cyclase Activating Peptide (PACAP)-induced migraine-like pain behavior. We also found that PACAP was able to dose-dependently lead to enzyme release from human mast cells via activation of MRGPRX2; the human homolog of MrgprB2. Using a transgenic MRGPRX2 mouse, we observed significant increases in PACAP-induced migraine-like pain behavior in MRGPRX2 + mice vs mice lacking the receptor. These results reveal both MrgprB2 and MRGPRX2 as important contributors to neuropeptide-induced migraine pain.