An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing

Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monos...

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Published inNature communications Vol. 13; no. 1; pp. 7645 - 12
Main Authors Xiong, Feng, Yang, Hong, Song, Yi-Ge, Qin, Hai-Bin, Zhang, Qing-Yang, Huang, Xian, Jing, Wei, Deng, Manfei, Liu, Yang, Liu, Zhixiang, Shen, Yin, Han, Yunyun, Lu, Youming, Xu, Xiangmin, Holmes, Todd C., Luo, Minmin, Zhao, Fei, Luo, Min-Hua, Zeng, Wen-Bo
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 10.12.2022
Nature Publishing Group
Nature Portfolio
Subjects
38/44
42
42/35
631/1647
631/378/3920
64/60
82/80
9/74
96/44
Circuits
Connectome
Gene mapping
Herpes simplex
Herpes viruses
Herpesvirus 1, Human - genetics
Humanities and Social Sciences
Injection
Mapping
multidisciplinary
Nerve Net
Neurons
Neurotoxicity
Payloads
Science
Science (multidisciplinary)
Toxicity
Tracing
Online AccessGet full text
ISSN2041-1723
2041-1723
DOI10.1038/s41467-022-35355-6

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Abstract Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129 Amp tracer system based on HSV-1 strain H129. The H129 Amp tracer system consists of two components: an H129-dTK-T2- pac Flox helper which assists H129 Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129 Amp tracer. These improvements of H129 Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129 Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129 Amp tracer system is a powerful tracing tool for revealing neuronal connectomes. Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an anterograde monosynaptic tracer system based on HSV amplicon, which shows fast tracing, bright labeling, low toxicity, input-defined postsynaptic neurons’ anterograde monosynaptic tracing feature, and has potential for functional mapping.
AbstractList Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an anterograde monosynaptic tracer system based on HSV amplicon, which shows fast tracing, bright labeling, low toxicity, input-defined postsynaptic neurons’ anterograde monosynaptic tracing feature, and has potential for functional mapping.
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129 tracer system based on HSV-1 strain H129. The H129 tracer system consists of two components: an H129-dTK-T2-pac helper which assists H129 tracer's propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129 tracer. These improvements of H129 tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129 tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129 tracer system is a powerful tracing tool for revealing neuronal connectomes.
Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an anterograde monosynaptic tracer system based on HSV amplicon, which shows fast tracing, bright labeling, low toxicity, input-defined postsynaptic neurons’ anterograde monosynaptic tracing feature, and has potential for functional mapping.
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer's propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer's propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129 Amp tracer system based on HSV-1 strain H129. The H129 Amp tracer system consists of two components: an H129-dTK-T2- pac Flox helper which assists H129 Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129 Amp tracer. These improvements of H129 Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129 Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129 Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129 Amp tracer system based on HSV-1 strain H129. The H129 Amp tracer system consists of two components: an H129-dTK-T2- pac Flox helper which assists H129 Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129 Amp tracer. These improvements of H129 Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129 Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129 Amp tracer system is a powerful tracing tool for revealing neuronal connectomes. Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an anterograde monosynaptic tracer system based on HSV amplicon, which shows fast tracing, bright labeling, low toxicity, input-defined postsynaptic neurons’ anterograde monosynaptic tracing feature, and has potential for functional mapping.
ArticleNumber 7645
Author Yang, Hong
Lu, Youming
Xu, Xiangmin
Han, Yunyun
Xiong, Feng
Luo, Minmin
Holmes, Todd C.
Huang, Xian
Liu, Yang
Zeng, Wen-Bo
Luo, Min-Hua
Song, Yi-Ge
Zhao, Fei
Deng, Manfei
Zhang, Qing-Yang
Liu, Zhixiang
Shen, Yin
Qin, Hai-Bin
Jing, Wei
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/36496505$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1523/JNEUROSCI.2158-19.2020
10.1007/s00429-014-0733-9
10.1186/s13024-021-00508-6
10.1016/j.cell.2015.07.015
10.1007/978-1-4939-2152-2_20
10.1074/jbc.R111.233981
10.1080/135502801300069656
10.1101/cshperspect.a013011
10.1152/ajpcell.2000.278.3.C619
10.1093/nar/gni035
10.1016/j.jchemneu.2019.101662
10.3390/ijms21165937
10.1016/j.neuron.2020.07.010
10.1038/s41467-017-01943-0
10.1038/s41593-018-0207-0
10.1016/j.neuron.2016.11.045
10.3389/fnana.2015.00080
10.1186/s13024-017-0179-7
10.1038/s41593-022-01068-8
10.1038/nature14600
10.1016/0042-6822(83)90097-1
10.1016/j.cell.2018.07.043
10.1038/nprot.2013.094
10.1016/j.cell.2017.06.014
10.1016/0092-8674(82)90035-6
10.1093/jnci/86.16.1209
10.1016/j.neuron.2016.09.021
10.1007/s12264-020-00614-3
10.1038/mt.2008.306
10.1038/sj.mt.6300273
10.1016/j.neuron.2007.01.033
10.1073/pnas.82.3.694
10.1128/JVI.80.7.3293-3300.2006
10.1038/sj.gt.3302617
10.1007/s12264-017-0099-0
10.3390/v1030594
10.1016/j.omtm.2021.03.020
10.1073/pnas.1110854108
10.1016/S0165-0270(02)00262-5
10.1089/hum.2004.15.1177
10.1016/j.neuron.2018.03.040
10.1016/j.ymthe.2004.06.641
10.1038/s41592-021-01319-9
10.1016/j.ebiom.2020.102838
10.1006/mthe.2000.0096
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References Tervo (CR44) 2016; 92
Beier (CR39) 2015; 162
Luo, Callaway, Svoboda (CR37) 2018; 98
Zingg (CR10) 2017; 93
Suzuki, Chiocca, Saeki (CR42) 2007; 15
Marconi, Fraefel, Epstein (CR26) 2015; 1254
Spaete, Frenkel (CR18) 1985; 82
Nassi, Cepko, Born, Beier (CR5) 2015; 9
Baker (CR35) 2020; 57
Yang (CR14) 2021; 37
Oehmig, Fraefel, Breakefield (CR27) 2004; 10
Li (CR47) 2018; 21
Weller, Coen (CR21) 2012; 4
Epstein (CR16) 2005; 12
Sandler (CR32) 2002; 121
Wojaczynski, Engel, Steren, Enquist, Patrick Card (CR30) 2015; 220
Osakada, Callaway (CR2) 2013; 8
Sun (CR36) 2004; 15
Jia (CR28) 1994; 86
Zhao (CR11) 2017; 33
Spaete, Frenkel (CR17) 1982; 30
Yang (CR9) 2022; 17
Soukupova (CR19) 2021; 21
Muylaert, Tang, Elias (CR22) 2011; 286
Tsai (CR13) 2022; 25
Li (CR15) 2020; 21
Schwarz (CR38) 2015; 524
Zhu (CR46) 2017; 8
CR45
Ciabatti, Gonzalez-Rueda, Mariotti, Morgese, Tripodi (CR3) 2017; 170
Wickersham (CR1) 2007; 53
Zingg, Peng, Huang, Tao, Zhang (CR12) 2020; 40
Suzuki (CR41) 2009; 17
Su (CR31) 2019; 100
Wang (CR34) 2000; 278
Beier (CR8) 2011; 108
Weller (CR24) 1985; 5
de Silva, Bowers (CR20) 2009; 1
Weir (CR25) 2001; 7
Xu (CR4) 2020; 107
Sena-Esteves, Saeki, Fraefel, Breakefield (CR33) 2000; 2
Stow, McMonagle (CR23) 1983; 130
Zeng (CR7) 2017; 12
Ren (CR40) 2018; 175
Suzuki, Kasai, Saeki (CR29) 2006; 80
Warming, Costantino, Court, Jenkins, Copeland (CR43) 2005; 33
Li (CR6) 2021; 18
M Suzuki (35355_CR29) 2006; 80
M Soukupova (35355_CR19) 2021; 21
WW Jia (35355_CR28) 1994; 86
M Suzuki (35355_CR41) 2009; 17
SK Weller (35355_CR24) 1985; 5
RR Spaete (35355_CR18) 1985; 82
NY Tsai (35355_CR13) 2022; 25
IR Wickersham (35355_CR1) 2007; 53
X Xu (35355_CR4) 2020; 107
D Li (35355_CR15) 2020; 21
P Marconi (35355_CR26) 2015; 1254
GJ Wojaczynski (35355_CR30) 2015; 220
L Luo (35355_CR37) 2018; 98
E Ciabatti (35355_CR3) 2017; 170
JP Weir (35355_CR25) 2001; 7
S Warming (35355_CR43) 2005; 33
DG Tervo (35355_CR44) 2016; 92
I Muylaert (35355_CR22) 2011; 286
H Zhu (35355_CR46) 2017; 8
E Li (35355_CR6) 2021; 18
B Zingg (35355_CR12) 2020; 40
VM Sandler (35355_CR32) 2002; 121
JJ Nassi (35355_CR5) 2015; 9
ND Stow (35355_CR23) 1983; 130
35355_CR45
P Su (35355_CR31) 2019; 100
X Li (35355_CR47) 2018; 21
AL Epstein (35355_CR16) 2005; 12
J Ren (35355_CR40) 2018; 175
Y Wang (35355_CR34) 2000; 278
SK Weller (35355_CR21) 2012; 4
F Osakada (35355_CR2) 2013; 8
H Yang (35355_CR14) 2021; 37
C Baker (35355_CR35) 2020; 57
H Yang (35355_CR9) 2022; 17
A Oehmig (35355_CR27) 2004; 10
LA Schwarz (35355_CR38) 2015; 524
B Zingg (35355_CR10) 2017; 93
M Suzuki (35355_CR42) 2007; 15
M Sena-Esteves (35355_CR33) 2000; 2
WB Zeng (35355_CR7) 2017; 12
M Sun (35355_CR36) 2004; 15
RR Spaete (35355_CR17) 1982; 30
KT Beier (35355_CR8) 2011; 108
KT Beier (35355_CR39) 2015; 162
F Zhao (35355_CR11) 2017; 33
S de Silva (35355_CR20) 2009; 1
References_xml – ident: CR45
– volume: 40
  start-page: 3250
  year: 2020
  end-page: 3267
  ident: CR12
  article-title: Synaptic specificity and application of anterograde transsynaptic AAV for probing neural circuitry
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.2158-19.2020
– volume: 220
  start-page: 1395
  year: 2015
  end-page: 1420
  ident: CR30
  article-title: The neuroinvasive profiles of H129 (herpes simplex virus type 1) recombinants with putative anterograde-only transneuronal spread properties
  publication-title: Brain Struct. Funct.
  doi: 10.1007/s00429-014-0733-9
– volume: 17
  start-page: 6
  year: 2022
  ident: CR9
  article-title: A novel H129-based anterograde monosynaptic tracer exhibits features of strong labeling intensity, high tracing efficiency, and reduced retrograde labeling
  publication-title: Mol. Neurodegener.
  doi: 10.1186/s13024-021-00508-6
– volume: 162
  start-page: 622
  year: 2015
  end-page: 634
  ident: CR39
  article-title: Circuit architecture of VTA dopamine neurons revealed by systematic input-output mapping
  publication-title: Cell
  doi: 10.1016/j.cell.2015.07.015
– volume: 1254
  start-page: 269
  year: 2015
  end-page: 293
  ident: CR26
  article-title: Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-2152-2_20
– volume: 286
  start-page: 15619
  year: 2011
  end-page: 15624
  ident: CR22
  article-title: Replication and recombination of herpes simplex virus DNA
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.R111.233981
– volume: 7
  start-page: 43
  year: 2001
  end-page: 51
  ident: CR25
  article-title: Infection of human NT2 cells and differentiated NT-neurons with herpes simplex virus and replication-incompetent herpes simplex virus vectors
  publication-title: J. Neurovirol.
  doi: 10.1080/135502801300069656
– volume: 4
  start-page: a013011
  year: 2012
  ident: CR21
  article-title: Herpes simplex viruses: mechanisms of DNA replication
  publication-title: Cold Spring Harb. Perspect. Biol.
  doi: 10.1101/cshperspect.a013011
– volume: 278
  start-page: C619
  year: 2000
  end-page: C626
  ident: CR34
  article-title: HSV-1 amplicon vectors are a highly efficient gene delivery system for skeletal muscle myoblasts and myotubes
  publication-title: Am. J. Physiol. Cell Physiol.
  doi: 10.1152/ajpcell.2000.278.3.C619
– volume: 33
  start-page: e36
  year: 2005
  ident: CR43
  article-title: Simple and highly efficient BAC recombineering using galK selection
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gni035
– volume: 100
  start-page: 101662
  year: 2019
  ident: CR31
  article-title: Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer
  publication-title: J. Chem. Neuroanat.
  doi: 10.1016/j.jchemneu.2019.101662
– volume: 21
  start-page: 5937
  year: 2020
  ident: CR15
  article-title: Anterograde neuronal circuit tracers derived from herpes simplex virus 1: development, application, and perspectives
  publication-title: Int. J. Mol. Sci
  doi: 10.3390/ijms21165937
– volume: 107
  start-page: 1029
  year: 2020
  end-page: 1047
  ident: CR4
  article-title: Viral vectors for neural circuit mapping and recent advances in trans-synaptic anterograde tracers
  publication-title: Neuron
  doi: 10.1016/j.neuron.2020.07.010
– volume: 8
  year: 2017
  ident: CR46
  article-title: Impairments of spatial memory in an Alzheimer’s disease model via degeneration of hippocampal cholinergic synapses
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-017-01943-0
– volume: 21
  start-page: 1239
  year: 2018
  end-page: 1250
  ident: CR47
  article-title: Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus
  publication-title: Nat. Neurosci.
  doi: 10.1038/s41593-018-0207-0
– volume: 93
  start-page: 33
  year: 2017
  end-page: 47
  ident: CR10
  article-title: AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense behaviors
  publication-title: Neuron
  doi: 10.1016/j.neuron.2016.11.045
– volume: 9
  start-page: 80
  year: 2015
  ident: CR5
  article-title: Neuroanatomy goes viral!
  publication-title: Front. Neuroanat.
  doi: 10.3389/fnana.2015.00080
– volume: 12
  start-page: 38
  year: 2017
  ident: CR7
  article-title: Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129
  publication-title: Mol. Neurodegener.
  doi: 10.1186/s13024-017-0179-7
– volume: 25
  start-page: 659
  year: 2022
  end-page: 674
  ident: CR13
  article-title: Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin
  publication-title: Nat. Neurosci.
  doi: 10.1038/s41593-022-01068-8
– volume: 524
  start-page: 88
  year: 2015
  end-page: 92
  ident: CR38
  article-title: Viral-genetic tracing of the input-output organization of a central noradrenaline circuit
  publication-title: Nature
  doi: 10.1038/nature14600
– volume: 130
  start-page: 427
  year: 1983
  end-page: 438
  ident: CR23
  article-title: Characterization of the TRS/IRS origin of DNA replication of herpes simplex virus type 1
  publication-title: Virology
  doi: 10.1016/0042-6822(83)90097-1
– volume: 175
  start-page: 472
  year: 2018
  end-page: 487 e420
  ident: CR40
  article-title: Anatomically defined and functionally distinct dorsal raphe serotonin sub-systems
  publication-title: Cell
  doi: 10.1016/j.cell.2018.07.043
– volume: 8
  start-page: 1583
  year: 2013
  end-page: 1601
  ident: CR2
  article-title: Design and generation of recombinant rabies virus vectors
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2013.094
– volume: 170
  start-page: 382
  year: 2017
  end-page: 392 e314
  ident: CR3
  article-title: Life-long genetic and functional access to neural circuits using self-inactivating rabies virus
  publication-title: Cell
  doi: 10.1016/j.cell.2017.06.014
– volume: 30
  start-page: 295
  year: 1982
  end-page: 304
  ident: CR17
  article-title: The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector
  publication-title: Cell
  doi: 10.1016/0092-8674(82)90035-6
– volume: 86
  start-page: 1209
  year: 1994
  end-page: 1215
  ident: CR28
  article-title: Selective destruction of gliomas in immunocompetent rats by thymidine kinase-defective herpes simplex virus type 1
  publication-title: J. Natl Cancer Inst.
  doi: 10.1093/jnci/86.16.1209
– volume: 92
  start-page: 372
  year: 2016
  end-page: 382
  ident: CR44
  article-title: A designer AAV variant permits efficient retrograde access to projection neurons
  publication-title: Neuron
  doi: 10.1016/j.neuron.2016.09.021
– volume: 37
  start-page: 701
  year: 2021
  end-page: 719
  ident: CR14
  article-title: HSV-1 H129-derived anterograde neural circuit tracers: improvements, production, and applications
  publication-title: Neurosci. Bull.
  doi: 10.1007/s12264-020-00614-3
– volume: 17
  start-page: 707
  year: 2009
  end-page: 715
  ident: CR41
  article-title: ICP0 inhibits the decrease of HSV amplicon-mediated transgene expression
  publication-title: Mol. Ther.
  doi: 10.1038/mt.2008.306
– volume: 15
  start-page: 2017
  year: 2007
  end-page: 2026
  ident: CR42
  article-title: Early STAT1 activation after systemic delivery of HSV amplicon vectors suppresses transcription of the vector-encoded transgene
  publication-title: Mol. Ther.
  doi: 10.1038/sj.mt.6300273
– volume: 53
  start-page: 639
  year: 2007
  end-page: 647
  ident: CR1
  article-title: Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons
  publication-title: Neuron
  doi: 10.1016/j.neuron.2007.01.033
– volume: 82
  start-page: 694
  year: 1985
  end-page: 698
  ident: CR18
  article-title: The herpes simplex virus amplicon: analyses of cis-acting replication functions
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.82.3.694
– volume: 80
  start-page: 3293
  year: 2006
  end-page: 3300
  ident: CR29
  article-title: Plasmid DNA sequences present in conventional herpes simplex virus amplicon vectors cause rapid transgene silencing by forming inactive chromatin
  publication-title: J. Virol.
  doi: 10.1128/JVI.80.7.3293-3300.2006
– volume: 12
  start-page: S154
  issue: Suppl 1
  year: 2005
  end-page: S158
  ident: CR16
  article-title: HSV-1-based amplicon vectors: design and applications
  publication-title: Gene Ther.
  doi: 10.1038/sj.gt.3302617
– volume: 33
  start-page: 348
  year: 2017
  end-page: 350
  ident: CR11
  article-title: Anterograde trans-synaptic tagging mediated by adeno-associated virus
  publication-title: Neurosci. Bull.
  doi: 10.1007/s12264-017-0099-0
– volume: 1
  start-page: 594
  year: 2009
  end-page: 629
  ident: CR20
  article-title: Herpes virus amplicon vectors
  publication-title: Viruses
  doi: 10.3390/v1030594
– volume: 21
  start-page: 399
  year: 2021
  end-page: 412
  ident: CR19
  article-title: Improvement of HSV-1 based amplicon vectors for a safe and long-lasting gene therapy in non-replicating cells
  publication-title: Mol. Ther. Methods Clin. Dev.
  doi: 10.1016/j.omtm.2021.03.020
– volume: 108
  start-page: 15414
  year: 2011
  end-page: 15419
  ident: CR8
  article-title: Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1110854108
– volume: 121
  start-page: 211
  year: 2002
  end-page: 219
  ident: CR32
  article-title: Modified herpes simplex virus delivery of enhanced GFP into the central nervous system
  publication-title: J. Neurosci. Methods
  doi: 10.1016/S0165-0270(02)00262-5
– volume: 15
  start-page: 1177
  year: 2004
  end-page: 1196
  ident: CR36
  article-title: Coexpression of tyrosine hydroxylase, GTP cyclohydrolase I, aromatic amino acid decarboxylase, and vesicular monoamine transporter 2 from a helper virus-free herpes simplex virus type 1 vector supports high-level, long-term biochemical and behavioral correction of a rat model of Parkinson’s disease
  publication-title: Hum. Gene Ther.
  doi: 10.1089/hum.2004.15.1177
– volume: 98
  start-page: 256
  year: 2018
  end-page: 281
  ident: CR37
  article-title: Genetic dissection of neural circuits: a decade of progress
  publication-title: Neuron
  doi: 10.1016/j.neuron.2018.03.040
– volume: 10
  start-page: 630
  year: 2004
  end-page: 643
  ident: CR27
  article-title: Update on herpesvirus amplicon vectors
  publication-title: Mol. Ther.
  doi: 10.1016/j.ymthe.2004.06.641
– volume: 18
  start-page: 1542
  year: 2021
  end-page: 1551
  ident: CR6
  article-title: Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D
  publication-title: Nat. Methods
  doi: 10.1038/s41592-021-01319-9
– volume: 5
  start-page: 930
  year: 1985
  end-page: 942
  ident: CR24
  article-title: Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis
  publication-title: Mol. Cell Biol.
– volume: 57
  start-page: 102838
  year: 2020
  ident: CR35
  article-title: Using recombination-dependent lethal mutations to stabilize reporter flaviviruses for rapid serodiagnosis and drug discovery
  publication-title: EBioMedicine
  doi: 10.1016/j.ebiom.2020.102838
– volume: 2
  start-page: 9
  year: 2000
  end-page: 15
  ident: CR33
  article-title: HSV-1 amplicon vectors–simplicity and versatility
  publication-title: Mol. Ther.
  doi: 10.1006/mthe.2000.0096
– volume: 12
  start-page: S154
  issue: Suppl 1
  year: 2005
  ident: 35355_CR16
  publication-title: Gene Ther.
  doi: 10.1038/sj.gt.3302617
– volume: 1254
  start-page: 269
  year: 2015
  ident: 35355_CR26
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-2152-2_20
– volume: 37
  start-page: 701
  year: 2021
  ident: 35355_CR14
  publication-title: Neurosci. Bull.
  doi: 10.1007/s12264-020-00614-3
– volume: 162
  start-page: 622
  year: 2015
  ident: 35355_CR39
  publication-title: Cell
  doi: 10.1016/j.cell.2015.07.015
– volume: 220
  start-page: 1395
  year: 2015
  ident: 35355_CR30
  publication-title: Brain Struct. Funct.
  doi: 10.1007/s00429-014-0733-9
– volume: 53
  start-page: 639
  year: 2007
  ident: 35355_CR1
  publication-title: Neuron
  doi: 10.1016/j.neuron.2007.01.033
– ident: 35355_CR45
– volume: 10
  start-page: 630
  year: 2004
  ident: 35355_CR27
  publication-title: Mol. Ther.
  doi: 10.1016/j.ymthe.2004.06.641
– volume: 33
  start-page: e36
  year: 2005
  ident: 35355_CR43
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gni035
– volume: 130
  start-page: 427
  year: 1983
  ident: 35355_CR23
  publication-title: Virology
  doi: 10.1016/0042-6822(83)90097-1
– volume: 98
  start-page: 256
  year: 2018
  ident: 35355_CR37
  publication-title: Neuron
  doi: 10.1016/j.neuron.2018.03.040
– volume: 8
  year: 2017
  ident: 35355_CR46
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-017-01943-0
– volume: 524
  start-page: 88
  year: 2015
  ident: 35355_CR38
  publication-title: Nature
  doi: 10.1038/nature14600
– volume: 278
  start-page: C619
  year: 2000
  ident: 35355_CR34
  publication-title: Am. J. Physiol. Cell Physiol.
  doi: 10.1152/ajpcell.2000.278.3.C619
– volume: 57
  start-page: 102838
  year: 2020
  ident: 35355_CR35
  publication-title: EBioMedicine
  doi: 10.1016/j.ebiom.2020.102838
– volume: 17
  start-page: 6
  year: 2022
  ident: 35355_CR9
  publication-title: Mol. Neurodegener.
  doi: 10.1186/s13024-021-00508-6
– volume: 7
  start-page: 43
  year: 2001
  ident: 35355_CR25
  publication-title: J. Neurovirol.
  doi: 10.1080/135502801300069656
– volume: 80
  start-page: 3293
  year: 2006
  ident: 35355_CR29
  publication-title: J. Virol.
  doi: 10.1128/JVI.80.7.3293-3300.2006
– volume: 175
  start-page: 472
  year: 2018
  ident: 35355_CR40
  publication-title: Cell
  doi: 10.1016/j.cell.2018.07.043
– volume: 30
  start-page: 295
  year: 1982
  ident: 35355_CR17
  publication-title: Cell
  doi: 10.1016/0092-8674(82)90035-6
– volume: 17
  start-page: 707
  year: 2009
  ident: 35355_CR41
  publication-title: Mol. Ther.
  doi: 10.1038/mt.2008.306
– volume: 21
  start-page: 1239
  year: 2018
  ident: 35355_CR47
  publication-title: Nat. Neurosci.
  doi: 10.1038/s41593-018-0207-0
– volume: 170
  start-page: 382
  year: 2017
  ident: 35355_CR3
  publication-title: Cell
  doi: 10.1016/j.cell.2017.06.014
– volume: 9
  start-page: 80
  year: 2015
  ident: 35355_CR5
  publication-title: Front. Neuroanat.
  doi: 10.3389/fnana.2015.00080
– volume: 1
  start-page: 594
  year: 2009
  ident: 35355_CR20
  publication-title: Viruses
  doi: 10.3390/v1030594
– volume: 2
  start-page: 9
  year: 2000
  ident: 35355_CR33
  publication-title: Mol. Ther.
  doi: 10.1006/mthe.2000.0096
– volume: 121
  start-page: 211
  year: 2002
  ident: 35355_CR32
  publication-title: J. Neurosci. Methods
  doi: 10.1016/S0165-0270(02)00262-5
– volume: 15
  start-page: 2017
  year: 2007
  ident: 35355_CR42
  publication-title: Mol. Ther.
  doi: 10.1038/sj.mt.6300273
– volume: 107
  start-page: 1029
  year: 2020
  ident: 35355_CR4
  publication-title: Neuron
  doi: 10.1016/j.neuron.2020.07.010
– volume: 15
  start-page: 1177
  year: 2004
  ident: 35355_CR36
  publication-title: Hum. Gene Ther.
  doi: 10.1089/hum.2004.15.1177
– volume: 18
  start-page: 1542
  year: 2021
  ident: 35355_CR6
  publication-title: Nat. Methods
  doi: 10.1038/s41592-021-01319-9
– volume: 33
  start-page: 348
  year: 2017
  ident: 35355_CR11
  publication-title: Neurosci. Bull.
  doi: 10.1007/s12264-017-0099-0
– volume: 4
  start-page: a013011
  year: 2012
  ident: 35355_CR21
  publication-title: Cold Spring Harb. Perspect. Biol.
  doi: 10.1101/cshperspect.a013011
– volume: 100
  start-page: 101662
  year: 2019
  ident: 35355_CR31
  publication-title: J. Chem. Neuroanat.
  doi: 10.1016/j.jchemneu.2019.101662
– volume: 5
  start-page: 930
  year: 1985
  ident: 35355_CR24
  publication-title: Mol. Cell Biol.
– volume: 86
  start-page: 1209
  year: 1994
  ident: 35355_CR28
  publication-title: J. Natl Cancer Inst.
  doi: 10.1093/jnci/86.16.1209
– volume: 40
  start-page: 3250
  year: 2020
  ident: 35355_CR12
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.2158-19.2020
– volume: 21
  start-page: 399
  year: 2021
  ident: 35355_CR19
  publication-title: Mol. Ther. Methods Clin. Dev.
  doi: 10.1016/j.omtm.2021.03.020
– volume: 8
  start-page: 1583
  year: 2013
  ident: 35355_CR2
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2013.094
– volume: 93
  start-page: 33
  year: 2017
  ident: 35355_CR10
  publication-title: Neuron
  doi: 10.1016/j.neuron.2016.11.045
– volume: 286
  start-page: 15619
  year: 2011
  ident: 35355_CR22
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.R111.233981
– volume: 82
  start-page: 694
  year: 1985
  ident: 35355_CR18
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.82.3.694
– volume: 21
  start-page: 5937
  year: 2020
  ident: 35355_CR15
  publication-title: Int. J. Mol. Sci
  doi: 10.3390/ijms21165937
– volume: 25
  start-page: 659
  year: 2022
  ident: 35355_CR13
  publication-title: Nat. Neurosci.
  doi: 10.1038/s41593-022-01068-8
– volume: 92
  start-page: 372
  year: 2016
  ident: 35355_CR44
  publication-title: Neuron
  doi: 10.1016/j.neuron.2016.09.021
– volume: 12
  start-page: 38
  year: 2017
  ident: 35355_CR7
  publication-title: Mol. Neurodegener.
  doi: 10.1186/s13024-017-0179-7
– volume: 108
  start-page: 15414
  year: 2011
  ident: 35355_CR8
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1110854108
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Snippet Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity...
Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an...
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SubjectTerms 38/44
42
42/35
631/1647
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64/60
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9/74
96/44
Circuits
Connectome
Gene mapping
Herpes simplex
Herpes viruses
Herpesvirus 1, Human - genetics
Humanities and Social Sciences
Injection
Mapping
multidisciplinary
Nerve Net
Neurons
Neurotoxicity
Payloads
Science
Science (multidisciplinary)
Toxicity
Tracing
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Title An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing
URI https://link.springer.com/article/10.1038/s41467-022-35355-6
https://www.ncbi.nlm.nih.gov/pubmed/36496505
https://www.proquest.com/docview/2748910804
https://www.proquest.com/docview/2753307460
https://pubmed.ncbi.nlm.nih.gov/PMC9741617
https://doaj.org/article/50eb8b5789094e9fb35682cd909e2b83
Volume 13
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