Determination of Estazolam in Plasma by High-Performance Liquid Chromatography with Solid-Phase Extraction

A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyano...

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Published in	Analytical Sciences Vol. 18; no. 5; pp. 525 - 528
Main Authors	SUGAWARA, Kazunobu, OTANI, Koichi, OHKUBO, Tadashi, OKUYAMA, Naoyuki, MIURA, Masatomo
Format	Journal Article
Language	English
Published	Tokyo The Japan Society for Analytical Chemistry 01.05.2002 Japan Society for Analytical Chemistry Nature Publishing Group
Subjects	Analysis Biological and medical sciences Calibration Chromatography, High Pressure Liquid - methods Estazolam - blood Estazolam - pharmacokinetics General pharmacology Humans Hypnotics and Sedatives - blood Hypnotics and Sedatives - pharmacokinetics Liquid chromatography Male Medical sciences Pharmacology. Drug treatments Reference Standards Reference Values Spectrophotometry, Ultraviolet Solid phase extraction Biological fluid Biochemical analysis HPLC chromatography Hypnotic Chemical enrichment Blood plasma Ultraviolet detector Estazolam Benzodiazepine derivatives Sedative Pharmacokinetics Quantitative analysis
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ISSN	0910-6340 1348-2246
DOI	10.2116/analsci.18.525

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Abstract	A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyanopropyl bonded-phase extraction. Chromatographic separation was achieved with a reversed-phase C8-5 column using a mobile phase of 0.5% potassium dihydrogenphosphate(pH 4.5)-acetonitrile (70:30, v/v). The determination of estazolam was possible in the concentration range of 1.0 - 200.0 ng/mL. The mean recovery of estazolam added to plasma was 96.1 ± 1.5% with coefficients of variation of less than 5.5%. This method is applicable for accurately monitoring the plasma level of estazolam in healthy subjects participating in scientific research.
AbstractList	A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyanopropyl bonded-phase extraction. Chromatographic separation was achieved with a reversed-phase C8-5 column using a mobile phase of 0.5% potassium dihydrogenphosphate(pH 4.5)-acetonitrile (70:30, v/v). The determination of estazolam was possible in the concentration range of 1.0 - 200.0 ng/mL. The mean recovery of estazolam added to plasma was 96.1 ± 1.5% with coefficients of variation of less than 5.5%. This method is applicable for accurately monitoring the plasma level of estazolam in healthy subjects participating in scientific research. A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyanopropyl bonded-phase extraction. Chromatographic separation was achieved with a reversed-phase C8-5 column using a mobile phase of 0.5% potassium dihydrogenphosphate(pH 4.5)-acetonitrile (70:30, v/v). The determination of estazolam was possible in the concentration range of 1.0 - 200.0 ng/mL. The mean recovery of estazolam added to plasma was 96.1 +/- 1.5% with coefficients of variation of less than 5.5%. This method is applicable for accurately monitoring the plasma level of estazolam in healthy subjects participating in scientific research.A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyanopropyl bonded-phase extraction. Chromatographic separation was achieved with a reversed-phase C8-5 column using a mobile phase of 0.5% potassium dihydrogenphosphate(pH 4.5)-acetonitrile (70:30, v/v). The determination of estazolam was possible in the concentration range of 1.0 - 200.0 ng/mL. The mean recovery of estazolam added to plasma was 96.1 +/- 1.5% with coefficients of variation of less than 5.5%. This method is applicable for accurately monitoring the plasma level of estazolam in healthy subjects participating in scientific research. A high-performance liquid chromatography (HPLC) assay was developed for the determination of estazolam in human plasma. Estazolam and alprazolam as an internal standard were detected by ultraviolet absorbance at 240 nm. Estazolam in plasma was extracted by a rapid and simple procedure based on cyanopropyl bonded-phase extraction. Chromatographic separation was achieved with a reversed-phase C8-5 column using a mobile phase of 0.5% potassium dihydrogenphosphate(pH 4.5)-acetonitrile (70:30, v/v). The determination of estazolam was possible in the concentration range of 1.0 - 200.0 ng/mL. The mean recovery of estazolam added to plasma was 96.1 +/- 1.5% with coefficients of variation of less than 5.5%. This method is applicable for accurately monitoring the plasma level of estazolam in healthy subjects participating in scientific research.
Author	OHKUBO, Tadashi OTANI, Koichi SUGAWARA, Kazunobu OKUYAMA, Naoyuki MIURA, Masatomo
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SubjectTerms	Analysis Biological and medical sciences Calibration Chromatography, High Pressure Liquid - methods Estazolam - blood Estazolam - pharmacokinetics General pharmacology Humans Hypnotics and Sedatives - blood Hypnotics and Sedatives - pharmacokinetics Liquid chromatography Male Medical sciences Pharmacology. Drug treatments Reference Standards Reference Values Spectrophotometry, Ultraviolet
Title	Determination of Estazolam in Plasma by High-Performance Liquid Chromatography with Solid-Phase Extraction
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