Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors - Wheeler, Emily C.; Vu, Anthony Q.; Einstein, Jaclyn M.; DiSalvo, Matthew; Ahmed, Noorsher; Van Nostrand, Eric L.; Shishkin, Alexander A.; Jin, Wenhao; Allbritton, Nancy L.; Yeo, Gene W. (Nature methods, 2020)
Keywords: 631/208; 631/61; 631/61/191; 631/80; 631/80/2373; Arrays; Automation; Binding proteins; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; Crafts; CRISPR; CRISPR-Cas Systems - genetics; Cytology; Cytoplasm - metabolism; Genetic screening; Granular materials; gRNA; Humans; Imaging; Learning algorithms; Life Sciences; Machine Learning; Mechanization; Microscopy, Confocal - methods; Morphology; Oxidative Stress - genetics; Phenotypes; Phenotyping; Protein Aggregates - genetics; Protein binding; Proteins; Proteomics; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; RNA-binding protein; RNA-Binding Proteins - genetics; Tissue Array Analysis - methods