Next-generation sequencing identifies micro-RNA–based biomarker panel for Kawasaki disease

• Published in: Journal of allergy and clinical immunology Vol. 138; no. 4; pp. 1227 - 1230
• Main Authors: Kuo, Ho-Chang, Hsieh, Kai-Sheng, Ming-Huey Guo, Mindy, Weng, Ken-Pen, Ger, Luo-Ping, Chan, Wen-Ching, Li, Sung-Chou
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2016; Elsevier Limited
• Subjects: Allergy and Immunology; Biomarkers; Case-Control Studies; Child, Preschool; Classification; Coronary vessels; Early Diagnosis; Female; Fever; Genetic Markers; High-Throughput Nucleotide Sequencing; Humans; Immunoglobulins; Infections; Kawasaki disease; Male; Males; MicroRNAs - blood; MicroRNAs - genetics; Mucocutaneous Lymph Node Syndrome - blood; Mucocutaneous Lymph Node Syndrome - diagnosis; Mucocutaneous Lymph Node Syndrome - genetics; Physicians; Researchers; Sequence Analysis, RNA; Studies; Support Vector Machine; Veins & arteries
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2016.04.050

Because males have a higher KD incidence rate than do females, we further investigated whether the 10 miRNAs are significantly differentially expressed between male and female subjects, causing bias when served as a disease biomarker. The expressions of the remaining 9 biomarker miRNAs were not sex specific. [...]we used the ΔCt values of the 10 miRNAs to develop a KD biomarker panel, training a Support Vector Machine (SVM) classification model, and diagnosing KD.