Periostin upregulates the effector functions of eosinophils

Eosinophils were obtained from healthy volunteers or allergic subjects with allergic rhinitis, and their adhesion to periostin or vascular cell adhesion molecule (VCAM)-1 was measured using residual eosinophil peroxidase assays (see this article's Methods section in the Online Repository at www...

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Published inJournal of allergy and clinical immunology Vol. 138; no. 5; pp. 1449 - 1452.e5
Main Authors Noguchi, Toru, Nakagome, Kazuyuki, Kobayashi, Takehito, Uchida, Yoshitaka, Soma, Tomoyuki, Nakamoto, Hidetomo, Nagata, Makoto
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.2016
Elsevier Limited
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Online AccessGet full text
ISSN0091-6749
1097-6825
1085-8725
1097-6825
DOI10.1016/j.jaci.2016.05.020

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Abstract Eosinophils were obtained from healthy volunteers or allergic subjects with allergic rhinitis, and their adhesion to periostin or vascular cell adhesion molecule (VCAM)-1 was measured using residual eosinophil peroxidase assays (see this article's Methods section in the Online Repository at www.jacionline.org). [...]the viability after 48-hour culture was much decreased to about 40% to 60% (Fig E1; 46.6% [without stimulation], 66.8% [+IL-5], or 47.0% [+periostin]). [...]in this study, we incubated eosinophils for 24 hours for cytokine measurement.
AbstractList Eosinophils were obtained from healthy volunteers or allergic subjects with allergic rhinitis, and their adhesion to periostin or vascular cell adhesion molecule (VCAM)-1 was measured using residual eosinophil peroxidase assays (see this article's Methods section in the Online Repository at www.jacionline.org). [...]the viability after 48-hour culture was much decreased to about 40% to 60% (Fig E1; 46.6% [without stimulation], 66.8% [+IL-5], or 47.0% [+periostin]). [...]in this study, we incubated eosinophils for 24 hours for cytokine measurement.
Author Noguchi, Toru
Uchida, Yoshitaka
Nakamoto, Hidetomo
Nakagome, Kazuyuki
Soma, Tomoyuki
Nagata, Makoto
Kobayashi, Takehito
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  givenname: Toru
  surname: Noguchi
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  givenname: Kazuyuki
  surname: Nakagome
  fullname: Nakagome, Kazuyuki
  email: nakagomek-tky@umin.ac.jp
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  givenname: Takehito
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  surname: Kobayashi
  fullname: Kobayashi, Takehito
  organization: Allergy Center, Saitama Medical University, Saitama, Japan
– sequence: 4
  givenname: Yoshitaka
  surname: Uchida
  fullname: Uchida, Yoshitaka
  organization: Allergy Center, Saitama Medical University, Saitama, Japan
– sequence: 5
  givenname: Tomoyuki
  surname: Soma
  fullname: Soma, Tomoyuki
  organization: Allergy Center, Saitama Medical University, Saitama, Japan
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  givenname: Hidetomo
  surname: Nakamoto
  fullname: Nakamoto, Hidetomo
  organization: Department of General Internal Medicine, Saitama Medical University, Saitama, Japan
– sequence: 7
  givenname: Makoto
  surname: Nagata
  fullname: Nagata, Makoto
  organization: Allergy Center, Saitama Medical University, Saitama, Japan
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  start-page: 6350
  year: 2010
  ident: 10.1016/j.jaci.2016.05.020_bibE6
  article-title: Human eosinophils recognize endogenous danger signal crystalline uric acid and produce proinflammatory cytokines mediated by autocrine ATP
  publication-title: J Immunol
  doi: 10.4049/jimmunol.0902673
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Snippet Eosinophils were obtained from healthy volunteers or allergic subjects with allergic rhinitis, and their adhesion to periostin or vascular cell adhesion...
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SubjectTerms Allergy and Immunology
Asthma
Cell adhesion & migration
Chemokines
Cytokines
Fibroblasts
Proteins
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Title Periostin upregulates the effector functions of eosinophils
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