Increased expression of α7nAChR in chronic rhinosinusitis: The intranasal cholinergic anti-inflammatory hypothesis

Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal sinuses. A parasympathetic-mediated anti-inflammatory reflex is known to have a pivotal role in the control of damage induced by immune response to injury and infectio...

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Published inAuris, nasus, larynx Vol. 43; no. 2; pp. 176 - 181
Main Authors Cerejeira, Rui, Fernandes, Susana, Pinto Moura, Carla
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ireland Ltd 01.04.2016
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ISSN0385-8146
1879-1476
DOI10.1016/j.anl.2015.08.011

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Abstract Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal sinuses. A parasympathetic-mediated anti-inflammatory reflex is known to have a pivotal role in the control of damage induced by immune response to injury and infection; acetylcholine released by peripheral nerves interacts with nicotinic acetylcholine receptor subunit α7 – α7nAChR – of innate immune cells, inhibiting pro-inflammatory signalling. This work aims to investigate whether cholinergic function is implicated in chronic rhinosinusitis. α7nAChR mRNA and protein levels were measured in nasal biopsy specimens of 14 patients with CRSwNP, 8 with CRSsNP and 10 control subjects, undergoing surgery. Gene expression levels of α7nAChR did not differ between groups; protein expression was significantly higher in CRSwNP than in CRSsNP (p=0.041), and both of these patient groups showed significant higher levels than controls (CRSwNP vs Controls – p=0.001; CRSsNP vs Controls – p=0.041). Elevated α7nAChR protein levels suggest that the cholinergic system is involved in the inflammatory response of chronic rhinosinusitis. This can shed light on both, the disease pathophysiology and the development of future treatment options.
AbstractList Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal sinuses. A parasympathetic-mediated anti-inflammatory reflex is known to have a pivotal role in the control of damage induced by immune response to injury and infection; acetylcholine released by peripheral nerves interacts with nicotinic acetylcholine receptor subunit α7 – α7nAChR – of innate immune cells, inhibiting pro-inflammatory signalling. This work aims to investigate whether cholinergic function is implicated in chronic rhinosinusitis. α7nAChR mRNA and protein levels were measured in nasal biopsy specimens of 14 patients with CRSwNP, 8 with CRSsNP and 10 control subjects, undergoing surgery. Gene expression levels of α7nAChR did not differ between groups; protein expression was significantly higher in CRSwNP than in CRSsNP (p=0.041), and both of these patient groups showed significant higher levels than controls (CRSwNP vs Controls – p=0.001; CRSsNP vs Controls – p=0.041). Elevated α7nAChR protein levels suggest that the cholinergic system is involved in the inflammatory response of chronic rhinosinusitis. This can shed light on both, the disease pathophysiology and the development of future treatment options.
OBJECTIVEChronic rhinosinusitis results from a dysfunctional host-environment interaction at the site of interface, in the nose and paranasal sinuses. A parasympathetic-mediated anti-inflammatory reflex is known to have a pivotal role in the control of damage induced by immune response to injury and infection; acetylcholine released by peripheral nerves interacts with nicotinic acetylcholine receptor subunit α7 - α7nAChR - of innate immune cells, inhibiting pro-inflammatory signalling. This work aims to investigate whether cholinergic function is implicated in chronic rhinosinusitis.METHODSα7nAChR mRNA and protein levels were measured in nasal biopsy specimens of 14 patients with CRSwNP, 8 with CRSsNP and 10 control subjects, undergoing surgery.RESULTSGene expression levels of α7nAChR did not differ between groups; protein expression was significantly higher in CRSwNP than in CRSsNP (p=0.041), and both of these patient groups showed significant higher levels than controls (CRSwNP vs Controls - p=0.001; CRSsNP vs Controls - p=0.041).CONCLUSIONElevated α7nAChR protein levels suggest that the cholinergic system is involved in the inflammatory response of chronic rhinosinusitis. This can shed light on both, the disease pathophysiology and the development of future treatment options.
Abstract Objective Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal sinuses. A parasympathetic-mediated anti-inflammatory reflex is known to have a pivotal role in the control of damage induced by immune response to injury and infection; acetylcholine released by peripheral nerves interacts with nicotinic acetylcholine receptor subunit α7 – α7nAChR – of innate immune cells, inhibiting pro-inflammatory signalling. This work aims to investigate whether cholinergic function is implicated in chronic rhinosinusitis. Methods α7nAChR mRNA and protein levels were measured in nasal biopsy specimens of 14 patients with CRSwNP, 8 with CRSsNP and 10 control subjects, undergoing surgery. Results Gene expression levels of α7nAChR did not differ between groups; protein expression was significantly higher in CRSwNP than in CRSsNP ( p = 0.041), and both of these patient groups showed significant higher levels than controls (CRSwNP vs Controls – p = 0.001; CRSsNP vs Controls – p = 0.041). Conclusion Elevated α7nAChR protein levels suggest that the cholinergic system is involved in the inflammatory response of chronic rhinosinusitis. This can shed light on both, the disease pathophysiology and the development of future treatment options.
Author Pinto Moura, Carla
Cerejeira, Rui
Fernandes, Susana
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Issue 2
Keywords Parasympathetic system
Inflammation
Nasal cavity
Rhinitis
Sinusitis
Language English
License Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
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Snippet Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal sinuses. A...
Abstract Objective Chronic rhinosinusitis results from a dysfunctional host–environment interaction at the site of interface, in the nose and paranasal...
Chronic rhinosinusitis results from a dysfunctional host-environment interaction at the site of interface, in the nose and paranasal sinuses. A...
OBJECTIVEChronic rhinosinusitis results from a dysfunctional host-environment interaction at the site of interface, in the nose and paranasal sinuses. A...
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StartPage 176
SubjectTerms Adolescent
Adult
Aged
alpha7 Nicotinic Acetylcholine Receptor - genetics
alpha7 Nicotinic Acetylcholine Receptor - metabolism
Case-Control Studies
Chronic Disease
Female
Gene Expression
Humans
Inflammation
Male
Middle Aged
Nasal cavity
Nasal Polyps - genetics
Nasal Polyps - metabolism
Otolaryngology
Parasympathetic Nervous System - metabolism
Parasympathetic system
Rhinitis
Rhinitis - genetics
Rhinitis - metabolism
RNA, Messenger - metabolism
Sinusitis
Sinusitis - genetics
Sinusitis - metabolism
Young Adult
Title Increased expression of α7nAChR in chronic rhinosinusitis: The intranasal cholinergic anti-inflammatory hypothesis
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https://www.ncbi.nlm.nih.gov/pubmed/26410356
https://www.proquest.com/docview/1769983379
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