Characterization of water-soluble shrimp allergens released during boiling

Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Sh...

Full description

Saved in:
Bibliographic Details
Published inJournal of allergy and clinical immunology Vol. 85; no. 6; pp. 1005 - 1013
Main Authors Lehrer, S.B., Ibanez, M.D., McCants, M.L., Daul, C.B., Morgan, J.E.
Format Journal Article
LanguageEnglish
Published United States Mosby, Inc 01.06.1990
Subjects
Online AccessGet full text
ISSN0091-6749
DOI10.1016/0091-6749(90)90044-5

Cover

Abstract Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. Although none of the sera from shrimp-tolerant individuals reacted, 12 14 sera (85.7%) from shrimp-sensitive subjects reacted with shrimp-water proteins with acid isoelectric points. Shrimp-water extract was fractionated by chromatofocusing with pH and NaCl gradients. A number of eluted ultraviolet-absorbing peaks contained allergens as determined by RAST inhibition. Isoelectrofocusing demonstrated many protein bands present in these peaks, some of which bound IgE from a RAST-positive sera pool. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.
AbstractList Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.
Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. Although none of the sera from shrimp-tolerant individuals reacted, 12 14 sera (85.7%) from shrimp-sensitive subjects reacted with shrimp-water proteins with acid isoelectric points. Shrimp-water extract was fractionated by chromatofocusing with pH and NaCl gradients. A number of eluted ultraviolet-absorbing peaks contained allergens as determined by RAST inhibition. Isoelectrofocusing demonstrated many protein bands present in these peaks, some of which bound IgE from a RAST-positive sera pool. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.
Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. Although none of the sera from shrimp-tolerant individuals reacted, 12/14 sera (85.7%) from shrimp-sensitive subjects reacted with shrimp-water proteins with acid isoelectric points. Shrimp-water extract was fractionated by chromatofocusing with pH and NaCl gradients. A number of eluted ultraviolet-absorbing peaks contained allergens as determined by RAST inhibition. Isoelectrofocusing demonstrated many protein bands present in these peaks, some of which bound IgE from a RAST-positive sera pool. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.
Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. Although none of the sera from shrimp-tolerant individuals reacted, 12/14 sera (85.7%) from shrimp-sensitive subjects reacted with shrimp-water proteins with acid isoelectric points. Shrimp-water extract was fractionated by chromatofocusing with pH and NaCl gradients. A number of eluted ultraviolet-absorbing peaks contained allergens as determined by RAST inhibition. Isoelectrofocusing demonstrated many protein bands present in these peaks, some of which bound IgE from a RAST-positive sera pool. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat). Both shrimp extracts contained acidic proteins (isoelectrofocusing) and demonstrated similar allergenic activity (RAST and RAST inhibition). Shrimp-water extract was analyzed further by immunoprinting with sera from 14 shrimp-sensitive, RAST-positive subjects, and six nonsensitive, RAST-negative individuals. Although none of the sera from shrimp-tolerant individuals reacted, 12/14 sera (85.7%) from shrimp-sensitive subjects reacted with shrimp-water proteins with acid isoelectric points. Shrimp-water extract was fractionated by chromatofocusing with pH and NaCl gradients. A number of eluted ultraviolet-absorbing peaks contained allergens as determined by RAST inhibition. Isoelectrofocusing demonstrated many protein bands present in these peaks, some of which bound IgE from a RAST-positive sera pool. These studies indicate that shrimp water is an excellent source of shrimp allergens, that chromatofocusing is a useful method for fractionation of shrimp allergens, and that shrimp allergens are generally protein molecules with acid isoelectric points.
Author Ibanez, M.D.
Lehrer, S.B.
Morgan, J.E.
McCants, M.L.
Daul, C.B.
Author_xml – sequence: 1
  givenname: S.B.
  surname: Lehrer
  fullname: Lehrer, S.B.
– sequence: 2
  givenname: M.D.
  surname: Ibanez
  fullname: Ibanez, M.D.
– sequence: 3
  givenname: M.L.
  surname: McCants
  fullname: McCants, M.L.
– sequence: 4
  givenname: C.B.
  surname: Daul
  fullname: Daul, C.B.
– sequence: 5
  givenname: J.E.
  surname: Morgan
  fullname: Morgan, J.E.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/2355151$$D View this record in MEDLINE/PubMed
BookMark eNqFUctOGzEU9SJVgNAfQCDNqmoXQ-3YnrFZIKGoD6pILGjWlsdzHVw549SeoaJfj_OABQuyujo-D1nnnKBRFzpA6JzgS4JJ9RVjScqqZvKzxF8kxoyVfISOX5-P0ElKf3DGVMgxGk8p54STY_Rr9qCjNj1E91_3LnRFsMU_nXGZgh8aD0V6iG61LrT3EJfQpSKCB52gLdohum5ZNMH5fE_RB6t9go_7O0GL799-z36W87sft7ObeWk4pX3ZNrKpcS2mzGKpTWutpg1YQqqqBsYtA41phrzhgrAp46QR1mhRkUqAYYZO0Kdd7jqGvwOkXq1cMuC97iAMSdVSUMprfFDIpGA8f-SgkHBBqymts_BiLxyaFbRqnZvR8Unt68z81Y43MaQUwSrj-m2tfdTOK4LVZi612UVtdlESq-1cimcze2N-iT9gO9vZrA5KL6NLanEvCa4YE5m83pGQB3l0EFUyDjoDrYtgetUG9376M1eptfw
CitedBy_id crossref_primary_10_1016_S1081_1206_10_60888_5
crossref_primary_10_1046_j_1365_2222_1998_00301_x
crossref_primary_10_4049_jimmunol_175_12_8354
crossref_primary_10_1007_BF02914471
crossref_primary_10_1016_0097_3165_93_90015_Z
crossref_primary_10_1016_0924_2244_93_90072_I
crossref_primary_10_1016_S0335_7457_97_80205_5
crossref_primary_10_1016_S1382_6689_97_10042_4
crossref_primary_10_1111_j_1398_9995_1995_tb02499_x
crossref_primary_10_3389_falgy_2021_769728
crossref_primary_10_1111_j_1399_3038_1992_tb00046_x
crossref_primary_10_1016_S1081_1206_10_62837_2
crossref_primary_10_1016_S1081_1206_10_62572_0
crossref_primary_10_1080_10408399609527762
crossref_primary_10_1080_10408399609527761
crossref_primary_10_1111_j_1365_2222_1993_tb00359_x
crossref_primary_10_1016_S0889_8561_05_70261_7
crossref_primary_10_1016_S0033_8389_22_00230_5
crossref_primary_10_1007_BF02539112
crossref_primary_10_1007_BF02802288
crossref_primary_10_4049_jimmunol_1601334
crossref_primary_10_1016_S0335_7457_01_80013_7
Cites_doi 10.1016/0091-6749(87)90121-7
10.1016/0091-6749(84)90256-2
10.1111/j.1365-2222.1978.tb00446.x
10.1159/000234418
10.4049/jimmunol.138.12.4169
10.1016/0091-6749(86)90173-9
10.1016/0091-6749(81)90041-5
10.1016/0019-2791(72)90112-7
10.1016/0091-6749(89)90474-0
10.1159/000232110
10.1056/NEJM198005153022005
10.1016/0091-6749(85)90724-9
10.1016/0091-6749(82)90079-3
10.1016/S0091-6749(86)80087-2
10.1016/0019-2791(75)90080-4
10.1016/0091-6749(87)90293-4
ContentType Journal Article
Copyright 1990
Copyright_xml – notice: 1990
DBID FBQ
AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7T5
F1W
H94
H95
L.G
7S9
L.6
7X8
DOI 10.1016/0091-6749(90)90044-5
DatabaseName AGRIS
CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Immunology Abstracts
ASFA: Aquatic Sciences and Fisheries Abstracts
AIDS and Cancer Research Abstracts
Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources
Aquatic Science & Fisheries Abstracts (ASFA) Professional
AGRICOLA
AGRICOLA - Academic
MEDLINE - Academic
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
AIDS and Cancer Research Abstracts
Aquatic Science & Fisheries Abstracts (ASFA) Professional
Immunology Abstracts
Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources
ASFA: Aquatic Sciences and Fisheries Abstracts
AGRICOLA
AGRICOLA - Academic
MEDLINE - Academic
DatabaseTitleList AIDS and Cancer Research Abstracts

MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: FBQ
  name: AGRIS
  url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN
  sourceTypes: Publisher
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EndPage 1013
ExternalDocumentID 2355151
10_1016_0091_6749_90_90044_5
US9106448
0091674990900445
Genre Research Support, U.S. Gov't, P.H.S
Comparative Study
Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: AI-19266
GroupedDBID ---
--K
--M
-~X
.1-
.55
.FO
.GJ
.XZ
.~1
0R~
1B1
1P~
1RT
1~.
1~5
354
3O-
4.4
457
4G.
53G
5GY
5RE
5VS
7-5
71M
8F7
8FE
8FH
8P~
9JM
AAAJQ
AABNK
AACTN
AAEDT
AAEDW
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AARKO
AATTM
AAXKI
AAXUO
ABBQC
ABFNM
ABJNI
ABLJU
ABMAC
ABMZM
ABOCM
ABWVN
ABXDB
ACDAQ
ACGFO
ACGFS
ACPRK
ACRLP
ACRPL
ADBBV
ADEZE
ADFRT
ADMUD
ADNMO
ADVLN
AEBSH
AEIPS
AEKER
AENEX
AFFNX
AFJKZ
AFRAH
AFRHN
AFTJW
AFXIZ
AGEKW
AGHFR
AGUBO
AGYEJ
AHHHB
AHMBA
AIEXJ
AIKHN
AITUG
AJRQY
AJUYK
AKRWK
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
ANKPU
ANZVX
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
BNPGV
BPHCQ
BVXVI
C45
CAG
CJTIS
COF
CS3
DU5
EBS
EFJIC
EJD
EO8
EO9
EP2
EP3
EX3
F5P
FDB
FEDTE
FGOYB
FIRID
FNPLU
FYGXN
G-2
G-Q
GBLVA
HDU
HMK
HMO
HVGLF
HZ~
IHE
J1W
J5H
K-O
KOM
L7B
LK8
LUGTX
M27
M41
MO0
N4W
N9A
O-L
O9-
O9~
OAUVE
OBH
ODZKP
OHH
OHT
OK0
OK1
OVD
OZT
P-8
P-9
P2P
PC.
PQQKQ
PROAC
Q38
R2-
RIG
ROL
RPZ
SAE
SCC
SDF
SDG
SDP
SEL
SES
SEW
SJN
SPCBC
SSH
SSI
SSZ
T5K
TEORI
TWZ
UGJ
UNMZH
UV1
WH7
WOW
WUQ
X7M
XFW
YOC
YQI
YQJ
Z5R
ZGI
ZXP
ZY1
~02
~G-
~KM
FBQ
AAFWJ
AAYWO
AAYXX
ACIEU
ACLOT
ACVFH
ADCNI
ADXHL
AEUPX
AFPUW
AGQPQ
AIGII
AIIUN
AKBMS
AKYEP
APXCP
CITATION
EFKBS
EFLBG
~HD
3V.
7RV
7X7
8C1
AFKRA
AFKWA
AJOXV
AMFUW
BBNVY
BENPR
BHPHI
CGR
CUY
CVF
ECM
EIF
HCIFZ
M2O
M7P
NPM
PKN
7T5
F1W
H94
H95
L.G
7S9
L.6
7X8
ID FETCH-LOGICAL-c533t-db9b707824f09acdffa3bef11667e45f4ea03f115b58142451b8fca86168ec4c3
ISSN 0091-6749
IngestDate Wed Oct 01 13:54:00 EDT 2025
Sat Sep 27 21:20:06 EDT 2025
Sat Sep 27 22:55:53 EDT 2025
Wed Feb 19 02:33:52 EST 2025
Wed Oct 01 05:25:59 EDT 2025
Thu Apr 24 22:58:09 EDT 2025
Thu Apr 03 09:45:26 EDT 2025
Sun Apr 06 06:53:35 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 6
Keywords PBS
UV
pI
IEF
INH-50
PAG
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c533t-db9b707824f09acdffa3bef11667e45f4ea03f115b58142451b8fca86168ec4c3
Notes 9106448
S30
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
PMID 2355151
PQID 15836237
PQPubID 23462
PageCount 9
ParticipantIDs proquest_miscellaneous_79833570
proquest_miscellaneous_49845782
proquest_miscellaneous_15836237
pubmed_primary_2355151
crossref_citationtrail_10_1016_0091_6749_90_90044_5
crossref_primary_10_1016_0091_6749_90_90044_5
fao_agris_US9106448
elsevier_sciencedirect_doi_10_1016_0091_6749_90_90044_5
ProviderPackageCode CITATION
AAYXX
PublicationCentury 1900
PublicationDate 1990-06-01
PublicationDateYYYYMMDD 1990-06-01
PublicationDate_xml – month: 06
  year: 1990
  text: 1990-06-01
  day: 01
PublicationDecade 1990
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Journal of allergy and clinical immunology
PublicationTitleAlternate J Allergy Clin Immunol
PublicationYear 1990
Publisher Mosby, Inc
Publisher_xml – name: Mosby, Inc
References Norman (BIB2) 1986; 77
Norman (BIB1) 1982; 69
Hoffman, Day, Miller (BIB9) 1981; 47
May, Bock (BIB15) 1978
Goldstein, Heiner (BIB3) 1970; 46
Cartier, Malo, Forrest (BIB16) 1984; 74
Daul, Morgan, McCants (BIB18) 1987; 80
Nagpal, Rejappa, Metcalf, Subba Rao (BIB14) 1989; 83
Hoffman (BIB6) 1975; 12
Nagpal, Metcalf, Subba Rao (BIB13) 1987; 138
Moroz, Yang (BIB7) 1980; 302
Sachs, Jones, Yunginger (BIB8) 1981; 67
Ceska, Lundkvist (BIB19) 1972; 9
Cartier, Malo, Ghezzo (BIB20) 1986; 78
Aukrust, Apold, Elsayed (BIB5) 1978; 57
Lehrer (BIB10) 1986; 57
Bush RF, Meier-Davis S, Lehrer SB, et al. Snow crab asthma: identification of allergens by immunoblotting [submitted].
Aas (BIB4) 1978; 8
Halmepuro, Salvaggio, Lehrer (BIB11) 1987; 84
Lehrer, McCants (BIB12) 1987; 80
Waring, deShazo, Daul (BIB17) 1985; 76
Hoffman (10.1016/0091-6749(90)90044-5_BIB9) 1981; 47
Halmepuro (10.1016/0091-6749(90)90044-5_BIB11) 1987; 84
Waring (10.1016/0091-6749(90)90044-5_BIB17) 1985; 76
Daul (10.1016/0091-6749(90)90044-5_BIB18) 1987; 80
Aas (10.1016/0091-6749(90)90044-5_BIB4) 1978; 8
Moroz (10.1016/0091-6749(90)90044-5_BIB7) 1980; 302
Nagpal (10.1016/0091-6749(90)90044-5_BIB13) 1987; 138
Hoffman (10.1016/0091-6749(90)90044-5_BIB6) 1975; 12
Norman (10.1016/0091-6749(90)90044-5_BIB1) 1982; 69
Norman (10.1016/0091-6749(90)90044-5_BIB2) 1986; 77
Lehrer (10.1016/0091-6749(90)90044-5_BIB10) 1986; 57
Ceska (10.1016/0091-6749(90)90044-5_BIB19) 1972; 9
Cartier (10.1016/0091-6749(90)90044-5_BIB20) 1986; 78
May (10.1016/0091-6749(90)90044-5_BIB15) 1978
Goldstein (10.1016/0091-6749(90)90044-5_BIB3) 1970; 46
Cartier (10.1016/0091-6749(90)90044-5_BIB16) 1984; 74
10.1016/0091-6749(90)90044-5_BIB21
Nagpal (10.1016/0091-6749(90)90044-5_BIB14) 1989; 83
Aukrust (10.1016/0091-6749(90)90044-5_BIB5) 1978; 57
Sachs (10.1016/0091-6749(90)90044-5_BIB8) 1981; 67
Lehrer (10.1016/0091-6749(90)90044-5_BIB12) 1987; 80
References_xml – volume: 9
  start-page: 1021
  year: 1972
  end-page: 1031
  ident: BIB19
  article-title: A new and simple radioimmunoassay method for determination of IgE
  publication-title: Immunochemistry
– volume: 69
  start-page: 1
  year: 1982
  end-page: 2
  ident: BIB1
  article-title: Quality control of allergen extracts [Editorial]
  publication-title: J Allergy Clin Immunol
– volume: 57
  start-page: 267
  year: 1986
  end-page: 272
  ident: BIB10
  article-title: The complex nature of food antigens: studies of cross-reacting crustacea allergens
  publication-title: Ann Allergy
– volume: 138
  start-page: 4169
  year: 1987
  end-page: 4174
  ident: BIB13
  article-title: Identification of shrimp-derived allergen as
  publication-title: J Immunol
– volume: 57
  start-page: 253
  year: 1978
  end-page: 262
  ident: BIB5
  article-title: Crossed-immunoelectrophoretic and crossed-radioimmunoelectrophoretic studies employing a model allergen from codfish
  publication-title: Intl Arch Allergy Appl Immunol
– volume: 80
  start-page: 133
  year: 1987
  end-page: 139
  ident: BIB12
  article-title: Reactivity of IgE antibodies with crustacea and oyster allergens: evidence for common allergenic structures
  publication-title: J Allergy Clin Immunol
– volume: 83
  start-page: 26
  year: 1989
  end-page: 36
  ident: BIB14
  article-title: Isolation and characterization of heat-stable allergens from shrimp (
  publication-title: J Allergy Clin Immunol
– volume: 77
  start-page: 405
  year: 1986
  end-page: 406
  ident: BIB2
  article-title: Why standardize extracts [Editorial]?
  publication-title: J Allergy Clin Immunol
– volume: 12
  start-page: 535
  year: 1975
  end-page: 538
  ident: BIB6
  article-title: The specificities of human IgE antibodies combining with cereal grains
  publication-title: Immunochemistry
– start-page: 1159
  year: 1978
  end-page: 1171
  ident: BIB15
  article-title: Adverse reactions to food due to hypersensitivity
  publication-title: Allergy: principles and practice
– volume: 80
  start-page: 716
  year: 1987
  end-page: 722
  ident: BIB18
  article-title: Crustacea allergy: immunologic evaluation of shrimp-allergic individuals
  publication-title: J Allergy Clin Immunol
– volume: 76
  start-page: 440
  year: 1985
  end-page: 445
  ident: BIB17
  article-title: Hypersensitivity reactions to ingested crustacea: clinical evaluation and diagnostic studies in shrimp-sensitive individuals
  publication-title: J Allergy Clin Immunol
– reference: Bush RF, Meier-Davis S, Lehrer SB, et al. Snow crab asthma: identification of allergens by immunoblotting [submitted].
– volume: 74
  start-page: 261
  year: 1984
  end-page: 269
  ident: BIB16
  article-title: Occupational asthma in snow crab-processing workers
  publication-title: I Allergy Clin Immunol
– volume: 84
  start-page: 165
  year: 1987
  end-page: 172
  ident: BIB11
  article-title: Studies of allergens present in crawfish and lobsters
  publication-title: Intl Arch Allergy Appl Immunol
– volume: 46
  start-page: 270
  year: 1970
  end-page: 291
  ident: BIB3
  article-title: Clinical and immunological perspectives in food sensitivity
  publication-title: J Allergy Clin Immunol
– volume: 67
  start-page: 27
  year: 1981
  end-page: 34
  ident: BIB8
  article-title: Isolation and partial characterization of a major peanut allergen
  publication-title: J Allergy Clin Immunol
– volume: 8
  start-page: 39
  year: 1978
  end-page: 50
  ident: BIB4
  article-title: The diagnosis of hypersensitivity to ingested foods
  publication-title: Clin Allergy
– volume: 302
  start-page: 1126
  year: 1980
  end-page: 1131
  ident: BIB7
  article-title: Kunitz soybean trypsin inhibitor
  publication-title: N Engl J Med
– volume: 47
  start-page: 17
  year: 1981
  end-page: 22
  ident: BIB9
  article-title: The major heat-stable allergen of shrimp
  publication-title: Ann Allergy
– volume: 78
  start-page: 344
  year: 1986
  end-page: 348
  ident: BIB20
  article-title: IgE sensitization in snowcrab-processing workers
  publication-title: J Allergy Clin Immunol
– volume: 80
  start-page: 133
  year: 1987
  ident: 10.1016/0091-6749(90)90044-5_BIB12
  article-title: Reactivity of IgE antibodies with crustacea and oyster allergens: evidence for common allergenic structures
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(87)90121-7
– volume: 74
  start-page: 261
  year: 1984
  ident: 10.1016/0091-6749(90)90044-5_BIB16
  article-title: Occupational asthma in snow crab-processing workers
  publication-title: I Allergy Clin Immunol
  doi: 10.1016/0091-6749(84)90256-2
– volume: 8
  start-page: 39
  year: 1978
  ident: 10.1016/0091-6749(90)90044-5_BIB4
  article-title: The diagnosis of hypersensitivity to ingested foods
  publication-title: Clin Allergy
  doi: 10.1111/j.1365-2222.1978.tb00446.x
– volume: 84
  start-page: 165
  year: 1987
  ident: 10.1016/0091-6749(90)90044-5_BIB11
  article-title: Studies of allergens present in crawfish and lobsters
  publication-title: Intl Arch Allergy Appl Immunol
  doi: 10.1159/000234418
– volume: 138
  start-page: 4169
  year: 1987
  ident: 10.1016/0091-6749(90)90044-5_BIB13
  article-title: Identification of shrimp-derived allergen as t RNA
  publication-title: J Immunol
  doi: 10.4049/jimmunol.138.12.4169
– volume: 77
  start-page: 405
  year: 1986
  ident: 10.1016/0091-6749(90)90044-5_BIB2
  article-title: Why standardize extracts [Editorial]?
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(86)90173-9
– volume: 46
  start-page: 270
  year: 1970
  ident: 10.1016/0091-6749(90)90044-5_BIB3
  article-title: Clinical and immunological perspectives in food sensitivity
  publication-title: J Allergy Clin Immunol
– volume: 67
  start-page: 27
  year: 1981
  ident: 10.1016/0091-6749(90)90044-5_BIB8
  article-title: Isolation and partial characterization of a major peanut allergen
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(81)90041-5
– ident: 10.1016/0091-6749(90)90044-5_BIB21
– start-page: 1159
  year: 1978
  ident: 10.1016/0091-6749(90)90044-5_BIB15
  article-title: Adverse reactions to food due to hypersensitivity
– volume: 9
  start-page: 1021
  year: 1972
  ident: 10.1016/0091-6749(90)90044-5_BIB19
  article-title: A new and simple radioimmunoassay method for determination of IgE
  publication-title: Immunochemistry
  doi: 10.1016/0019-2791(72)90112-7
– volume: 57
  start-page: 267
  year: 1986
  ident: 10.1016/0091-6749(90)90044-5_BIB10
  article-title: The complex nature of food antigens: studies of cross-reacting crustacea allergens
  publication-title: Ann Allergy
– volume: 83
  start-page: 26
  year: 1989
  ident: 10.1016/0091-6749(90)90044-5_BIB14
  article-title: Isolation and characterization of heat-stable allergens from shrimp (Penaeus indicus)
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(89)90474-0
– volume: 57
  start-page: 253
  year: 1978
  ident: 10.1016/0091-6749(90)90044-5_BIB5
  article-title: Crossed-immunoelectrophoretic and crossed-radioimmunoelectrophoretic studies employing a model allergen from codfish
  publication-title: Intl Arch Allergy Appl Immunol
  doi: 10.1159/000232110
– volume: 302
  start-page: 1126
  year: 1980
  ident: 10.1016/0091-6749(90)90044-5_BIB7
  article-title: Kunitz soybean trypsin inhibitor
  publication-title: N Engl J Med
  doi: 10.1056/NEJM198005153022005
– volume: 76
  start-page: 440
  year: 1985
  ident: 10.1016/0091-6749(90)90044-5_BIB17
  article-title: Hypersensitivity reactions to ingested crustacea: clinical evaluation and diagnostic studies in shrimp-sensitive individuals
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(85)90724-9
– volume: 69
  start-page: 1
  year: 1982
  ident: 10.1016/0091-6749(90)90044-5_BIB1
  article-title: Quality control of allergen extracts [Editorial]
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(82)90079-3
– volume: 47
  start-page: 17
  year: 1981
  ident: 10.1016/0091-6749(90)90044-5_BIB9
  article-title: The major heat-stable allergen of shrimp
  publication-title: Ann Allergy
– volume: 78
  start-page: 344
  year: 1986
  ident: 10.1016/0091-6749(90)90044-5_BIB20
  article-title: IgE sensitization in snowcrab-processing workers
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/S0091-6749(86)80087-2
– volume: 12
  start-page: 535
  year: 1975
  ident: 10.1016/0091-6749(90)90044-5_BIB6
  article-title: The specificities of human IgE antibodies combining with cereal grains
  publication-title: Immunochemistry
  doi: 10.1016/0019-2791(75)90080-4
– volume: 80
  start-page: 716
  year: 1987
  ident: 10.1016/0091-6749(90)90044-5_BIB18
  article-title: Crustacea allergy: immunologic evaluation of shrimp-allergic individuals
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/0091-6749(87)90293-4
SSID ssj0009389
Score 1.5095706
Snippet Water-soluble shrimp allergens released during boiling (shrimp water) were characterized and compared to allergen extracts from boiled shrimp (shrimp meat)....
SourceID proquest
pubmed
crossref
fao
elsevier
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 1005
SubjectTerms ALERGENOS
ALERGIA ALIMENTARIA
ALLERGENE
ALLERGENS
Allergens - analysis
ALLERGIE ALIMENTAIRE
analysis
Animals
Antibody Specificity
BOILING
CAMARON DE MAR
CREVETTE
CUISSON A L'EAU
Decapoda
Decapoda (Crustacea)
Decapoda (Crustacea) - immunology
EBULLICION
EXTRACTION
FOOD ALLERGIES
Humans
HYPERSENSITIVITY
Immunoglobulin E
Immunoglobulin E - immunology
immunologic techniques
IMMUNOLOGICAL TECHNIQUES
immunology
IMMUNOPRINTING
Marine
PRAWNS
Radioallergosorbent Test
RADIOIMMUNOASSAY
RAST
REACCIONES ALERGICAS
REACTION ALLERGIQUE
seafoods
shrimp
SHRIMP MEAT
SHRIMP WATER
Solubility
TECHNIQUE IMMUNOLOGIQUE
TECHNIQUE RADIOIMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
TECNICAS RADIOINMUNOLOGICAS
Water
Title Characterization of water-soluble shrimp allergens released during boiling
URI https://dx.doi.org/10.1016/0091-6749(90)90044-5
https://www.ncbi.nlm.nih.gov/pubmed/2355151
https://www.proquest.com/docview/15836237
https://www.proquest.com/docview/49845782
https://www.proquest.com/docview/79833570
Volume 85
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVLSH
  databaseName: Elsevier Journals
  issn: 0091-6749
  databaseCode: AKRWK
  dateStart: 19710101
  customDbUrl:
  isFulltext: true
  mediaType: online
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0009389
  providerName: Library Specific Holdings
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLZYJ9BeEAwmyjUPTAIhQy7OxY9TAY2N8sBWsTfLV1Zpa6pehLRfz3FsJ5QRDXiJWie2FZ8v9hfnfOcg9DKBZZ2bQmGZKoJJWUpMVWqw1pUmpRIi442X75ficEKOzvKzzk-3UZesxFt59Uddyf9YFcrArlYl-w-WbRuFAvgN9oUjWBiOf2XjURtt-aplfj-APC6w7ddqopbni-nl_I1NmGJVlssmRwosXCroE0U9vQir13WO6uq5EE2thnJqJSUbu_Gf9bnPin3SZXH-JPjMbU-PO7fisRzxxnPDlrb7zu_5utmJDjmglZflxZ23VJhZaYKL0oUfDTOrS8bjEfTrNJnEjdb6-vztthLaxvbtZ9B9u2LGhOCNKmCJ-WVj1xQoU-Kj1m6GzvZnttB2WhZFOkDbB8dfvx13QZmzyr0e-e6CtDIp3rVlr2j82ne_g277FvtIzJbhdf-rSkNZTu-hu96O0YEDzn10S8920Z2x96Z4gI5-x09Um2gDP5HDT9TiJwr4iRx-Io-fh2jy8cPp6BD73BpYAsFfYSWoaIIlEhNTLpUxPBPaJElRlJrkhmgeZ_A3F3llxZB5IiojeVUkRaUlkdkeGszqmX6EIsUp0UQISjnQf2Wq2KRcVUSlPMtiKYYoC4PFpA88b_OfXLDgYWgHm9nBZjRmzWCzfIhwW2vuAq_ccH0Z7MA8eXSkkAG2bqi5C2Zj_DusqmxyAvzZ7lkM0YtgRgYzrv2MBk9NvV6yJK-A9WVl_xWEVsSmiei_oqRW7FjGQ7TnENLeo0fY474TT9BO9_w9RYPVYq2fAS9eiece3j8B7civxg
linkProvider Library Specific Holdings
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+of+water-soluble+shrimp+allergens+released+during+boiling&rft.jtitle=Journal+of+allergy+and+clinical+immunology&rft.au=Lehrer%2C+S+B&rft.au=Ibanez%2C+M+D&rft.au=McCants%2C+M+L&rft.au=Daul%2C+C+B&rft.date=1990-06-01&rft.issn=0091-6749&rft.volume=85&rft.issue=6&rft.spage=1005&rft_id=info:doi/10.1016%2F0091-6749%2890%2990044-5&rft_id=info%3Apmid%2F2355151&rft.externalDocID=2355151
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0091-6749&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0091-6749&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0091-6749&client=summon