A pilot study optimizing metabolomic and lipidomic acquisition in serum for biomarker discovery in nonalcoholic fatty liver disease - He, Dandan; Su, Yang; Meng, Duanyue; Wang, Xinmiao; Wang, Jun; Ye, Hui (Journal of mass spectrometry and advances in the clinical lab, 2021)
Keywords: Acylcarnitines; Lipids; Metabolic biomarkers; Metabolomics; NAFLD; Special issue on Lipidomics; Metabolomics; NAFLD; amide-pos; amide-neg; HFD; Lipids; DGs; RPLC; EICs; MGs; T3-neg; Acylcarnitines; HILIC; T3-pos; PEs; PCs; MS; ESI− and ESI; OPLS-DA; ROC; HCC; PKC; ACN; NASH; MCD; TIC; LE; FA; Metabolic biomarkers; VIP; FC; LPC; DGs, diacylglycerols; EICs, extracted ion chromatograms; MS, mass spectrometry; T3-neg, T3 column-based reverse phase separation plus the negative ion mode; FA, Formic acid; LPC, lysophosphatidylcholine; MGs, monoacylglycerols; NAFLD, non-alcoholic fatty liver disease; ACN, acetonitrile; HFD, high-fat diet; MCD, methionine-choline-deficient; T3-pos, T3 column-based reverse phase separation plus the positive ion mode; PKC∊, protein kinase C; PEs, phosphatidylethanolamines; TIC, total ion chromatogram; ESI− and ESI+, ionization polarity modes; HILIC, hydrophilic interaction chromatography; OPLS-DA, orthogonal partial least square discriminant analysis; NASH, non-alcoholic steatohepatitis; amide-neg, amide column-based HILIC separation plus the negative ion mode; PCs, phosphorylcholines; ROC, receiver operating characteristic; FC, fold change; LE, Leucine enkephalin; RPLC, reversed-phase liquid chromatography; VIP, variable importance; amide-pos, amide column-based HILIC separation plus the positive ion mode; HCC, hepatocellular carcinoma