Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria
Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight...
Saved in:
| Published in | Frontiers in microbiology Vol. 9; p. 3052 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
Frontiers Media S.A
12.12.2018
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1664-302X 1664-302X |
| DOI | 10.3389/fmicb.2018.03052 |
Cover
| Abstract | Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn
transposon delivery plasmid, and a Tn
transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn
transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria-bacteria, bacteria-host, and bacteria-environment interactions. |
|---|---|
| AbstractList | Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green–yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn
5
transposon delivery plasmid, and a Tn
7
transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn
5
transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria–bacteria, bacteria–host, and bacteria–environment interactions. Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn5 transposon delivery plasmid, and a Tn7 transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn5 transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria-bacteria, bacteria-host, and bacteria-environment interactions.Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn5 transposon delivery plasmid, and a Tn7 transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn5 transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria-bacteria, bacteria-host, and bacteria-environment interactions. Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn transposon delivery plasmid, and a Tn transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria-bacteria, bacteria-host, and bacteria-environment interactions. Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green–yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn5 transposon delivery plasmid, and a Tn7 transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn5 transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria–bacteria, bacteria–host, and bacteria–environment interactions. |
| Author | Jun, Hyunwoo Schlechter, Rudolf O. Oso, Simisola Dobson, Renwick C. J. Boyd, Erica Muñoz-Lintz, Dian A. Remus-Emsermann, Mitja N. P. Remus, Daniela M. Bernach, Michał |
| AuthorAffiliation | 4 Protein Science & Engineering, Callaghan Innovation, School of Biological Sciences, University of Canterbury , Christchurch , New Zealand 1 School of Biological Sciences, University of Canterbury , Christchurch , New Zealand 3 Bio21 Molecular Science and Biotechnology Institute, Department of Biochemistry and Molecular Biology, The University of Melbourne , Parkville, VIC , Australia 2 Biomolecular Interaction Centre, University of Canterbury , Christchurch , New Zealand |
| AuthorAffiliation_xml | – name: 4 Protein Science & Engineering, Callaghan Innovation, School of Biological Sciences, University of Canterbury , Christchurch , New Zealand – name: 3 Bio21 Molecular Science and Biotechnology Institute, Department of Biochemistry and Molecular Biology, The University of Melbourne , Parkville, VIC , Australia – name: 1 School of Biological Sciences, University of Canterbury , Christchurch , New Zealand – name: 2 Biomolecular Interaction Centre, University of Canterbury , Christchurch , New Zealand |
| Author_xml | – sequence: 1 givenname: Rudolf O. surname: Schlechter fullname: Schlechter, Rudolf O. – sequence: 2 givenname: Hyunwoo surname: Jun fullname: Jun, Hyunwoo – sequence: 3 givenname: Michał surname: Bernach fullname: Bernach, Michał – sequence: 4 givenname: Simisola surname: Oso fullname: Oso, Simisola – sequence: 5 givenname: Erica surname: Boyd fullname: Boyd, Erica – sequence: 6 givenname: Dian A. surname: Muñoz-Lintz fullname: Muñoz-Lintz, Dian A. – sequence: 7 givenname: Renwick C. J. surname: Dobson fullname: Dobson, Renwick C. J. – sequence: 8 givenname: Daniela M. surname: Remus fullname: Remus, Daniela M. – sequence: 9 givenname: Mitja N. P. surname: Remus-Emsermann fullname: Remus-Emsermann, Mitja N. P. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30631309$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1Ul1rHCEUlZLQpGne-1R87Mts1Rkd56WQLPlYCCSUFPomjh8bg6NbnS3pW-lfyD_sL6mzmy1JoT7oVc85l3vveQP2QgwGgHcYzeqadx_t4FQ_IwjzGaoRJa_AIWasqWpEvu49iw_Acc73qKwGkbK_Bgc1YjWuUXcIfs3vUhzk6BQ8lWo0yUn4--cjPIGnKUoNL2Meq88yLA288TIPTkMZNNywYi5MDxchmzS6GOBtjL6PD9DGBM_9OiaTlQkjvElxNC7As4dVecoTtNx2-d6CfSt9NsdP5xH4cn52O7-srq4vFvOTq0pRwseK86Yvkeo0txprqvpGY6JYa7lG2PLW4pYz3fdt33PcdFLRjkmrcIuopbarj8Biq6ujvBer5AaZfogondg8xLQUstShvBEUMY4J4x0ltqGsk43CFktjuEK41rhofdpqrdb9YPRUZZL-hejLn-DuxDJ-F6XvFLekCHx4Ekjx29rkUQyuNMt7GUxcZ0Fw25Upt4QW6Pvnuf4m2Q2xANgWoFLMORkrlBvlNJGS2nmBkZgMIzaGEZNhxMYwhYj-Ie60_0v5A3huxqk |
| CitedBy_id | crossref_primary_10_1128_mbio_01999_23 crossref_primary_10_3390_cells11050873 crossref_primary_10_1038_s41467_024_47416_z crossref_primary_10_1128_mSphere_01331_20 crossref_primary_10_1128_spectrum_02988_23 crossref_primary_10_3390_antibiotics14020152 crossref_primary_10_1007_s00239_023_10103_6 crossref_primary_10_1016_j_copbio_2019_03_012 crossref_primary_10_1371_journal_pgen_1011572 crossref_primary_10_1111_1462_2920_16267 crossref_primary_10_1094_PHYTO_01_23_0016_IA crossref_primary_10_3390_bacteria1010006 crossref_primary_10_1094_PDIS_05_23_0837_RE crossref_primary_10_1073_pnas_2108653119 crossref_primary_10_3390_bios12020064 crossref_primary_10_1126_sciadv_abi7159 crossref_primary_10_1016_j_microb_2024_100089 crossref_primary_10_21769_BioProtoc_3199 crossref_primary_10_1128_aem_00317_23 crossref_primary_10_7567_1347_4065_ab0498 crossref_primary_10_1016_j_csbj_2020_05_023 crossref_primary_10_1128_spectrum_04105_22 crossref_primary_10_1093_ismeco_ycaf031 crossref_primary_10_1111_1462_2920_70025 crossref_primary_10_1371_journal_pcbi_1009461 crossref_primary_10_1093_jambio_lxaf025 crossref_primary_10_31083_FBL23223 crossref_primary_10_3389_fpls_2022_1104905 crossref_primary_10_1063_5_0057787 crossref_primary_10_3390_microorganisms8040464 crossref_primary_10_1038_s41467_024_51944_z crossref_primary_10_12688_f1000research_17652_1 crossref_primary_10_1128_aem_02078_23 crossref_primary_10_3390_microorganisms11010193 crossref_primary_10_1007_s00248_024_02381_4 crossref_primary_10_1111_1758_2229_12934 crossref_primary_10_1038_s41598_024_56443_1 crossref_primary_10_1038_s41598_019_50983_7 crossref_primary_10_3390_cimb47010017 crossref_primary_10_1016_j_plaphy_2023_01_010 crossref_primary_10_1038_s41598_022_13214_0 crossref_primary_10_1038_s41396_022_01271_2 crossref_primary_10_1093_plcell_koac286 crossref_primary_10_1016_j_eti_2022_102910 crossref_primary_10_1016_j_chembiol_2022_02_007 crossref_primary_10_1038_s41396_023_01428_7 crossref_primary_10_1016_j_biortech_2021_125568 crossref_primary_10_1111_1750_3841_17294 crossref_primary_10_1021_acssynbio_0c00280 crossref_primary_10_1186_s40168_024_01792_2 crossref_primary_10_1128_AEM_00982_21 crossref_primary_10_1128_mSphere_00321_21 crossref_primary_10_1038_s41596_021_00582_6 crossref_primary_10_1111_tpj_14654 crossref_primary_10_1021_acsbiomaterials_4c00101 crossref_primary_10_1016_j_cub_2024_01_029 crossref_primary_10_1021_acsami_3c14387 |
| Cites_doi | 10.1007/978-1-61779-483-4_3 10.1016/j.cell.2017.03.041 10.1016/j.syapm.2012.03.004 10.1099/00221287-147-9-2529 10.1128/AEM.01084-09 10.1111/1758-2229.12040 10.1128/mBio.00315-10 10.1128/microbiolspec.MDNA3-0010-2014 10.1094/MPMI.2000.13.11.1243 10.1128/JB.01288-12 10.1007/s00253-011-3618-3 10.3389/fenvs.2018.00061 10.1111/nph.15054 10.1186/1471-2180-6-39 10.1128/jb.172.11.6568-6572.1990 10.3389/fmicb.2014.00730 10.1002/mbo3.171 10.1016/j.mimet.2005.02.011 10.3389/fmicb.2017.02669 10.1093/femsle/fnw178 10.1093/jac/dki255 10.1038/nmeth.4074 10.1038/srep20889 10.1128/AEM.71.1.451-459.2005 10.1111/j.1462-2920.2005.00880.x 10.1021/sb500221v 10.1038/msb.2011.58 10.1007/b102216 10.1073/pnas.0504930102 10.1016/j.micres.2018.07.007 10.1021/bi0622874 10.1038/nature16192 10.1038/nmeth.1318 10.1128/AEM.00133-11 10.1093/nar/gky726 10.1111/j.1574-6976.2011.00289.x 10.1094/MPMI-03-15-0054-TA 10.1094/MPMI-23-4-0384 10.1016/S0140-6736(01)05321-1 10.3389/fmicb.2017.02491 10.1371/journal.pone.0028674 10.1126/science.1225829 10.1146/annurev.genet.42.110807.091656 10.1038/nmeth.2888 10.1038/nbt1183-784 10.1371/journal.pone.0006232 10.3389/fmicb.2018.00145 10.1371/journal.pone.0146827 10.1371/journal.pone.0153158 10.1094/MPMI.2000.13.11.1170 10.1007/s002480000057 10.1016/0167-7799(91)90089-Z 10.1534/genetics.111.131433 10.1139/w98-020 10.1111/j.1462-2920.2012.02715.x 10.1038/nprot.2006.24 10.1016/S0378-1119(97)00197-2 10.5281/ZENODO.1244328 10.1111/j.1574-6968.2010.01916.x 10.1186/s40793-016-0190-6 10.1038/84397 10.1111/j.1462-2920.2004.00605.x 10.1111/1462-2920.12482 10.1038/nmeth.2019 10.1128/genomeA.00036-13 10.1128/AEM.71.9.5484-5493.2005 10.1038/ncomms1738 10.1016/j.plasmid.2013.07.007 10.1128/AEM.64.6.2240-2246.1998 10.1128/JB.01536-06 10.1038/nature11913 |
| ContentType | Journal Article |
| Copyright | Copyright © 2018 Schlechter, Jun, Bernach, Oso, Boyd, Muñoz-Lintz, Dobson, Remus and Remus-Emsermann. 2018 Schlechter, Jun, Bernach, Oso, Boyd, Muñoz-Lintz, Dobson, Remus and Remus-Emsermann |
| Copyright_xml | – notice: Copyright © 2018 Schlechter, Jun, Bernach, Oso, Boyd, Muñoz-Lintz, Dobson, Remus and Remus-Emsermann. 2018 Schlechter, Jun, Bernach, Oso, Boyd, Muñoz-Lintz, Dobson, Remus and Remus-Emsermann |
| DBID | AAYXX CITATION NPM 7X8 5PM DOA |
| DOI | 10.3389/fmicb.2018.03052 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Open Access Full Text url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1664-302X |
| ExternalDocumentID | oai_doaj_org_article_50681268952f4569a4c1f1aee8c013d1 PMC6315172 30631309 10_3389_fmicb_2018_03052 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: Marsden Fund grantid: UOC1704 |
| GroupedDBID | 53G 5VS 9T4 AAFWJ AAKDD AAYXX ACGFO ACGFS ACXDI ADBBV ADRAZ AENEX AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV CITATION DIK ECGQY GROUPED_DOAJ GX1 HYE KQ8 M48 M~E O5R O5S OK1 PGMZT RNS RPM IAO IEA IHR IPNFZ NPM RIG 7X8 5PM |
| ID | FETCH-LOGICAL-c528t-884bc52c9d8fd1d5cb4d12c67f8d01f87f1786dbb7bb8149ac596afc1705f5f93 |
| IEDL.DBID | M48 |
| ISSN | 1664-302X |
| IngestDate | Wed Aug 27 01:26:47 EDT 2025 Thu Aug 21 14:34:39 EDT 2025 Fri Sep 05 11:46:05 EDT 2025 Thu Jan 02 23:01:25 EST 2025 Tue Jul 01 00:44:27 EDT 2025 Thu Apr 24 23:08:46 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | transposon tagging fluorophore Tn5 Tn7 fluorescent labeling |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c528t-884bc52c9d8fd1d5cb4d12c67f8d01f87f1786dbb7bb8149ac596afc1705f5f93 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work Edited by: Haiwei Luo, The Chinese University of Hong Kong, China Reviewed by: Yongtao Zhu, Minnesota State University, Mankato, United States; Beatriz Quiñones, United States Department of Agriculture – Agricultural Research Service, United States This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.3389/fmicb.2018.03052 |
| PMID | 30631309 |
| PQID | 2179338725 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_50681268952f4569a4c1f1aee8c013d1 pubmedcentral_primary_oai_pubmedcentral_nih_gov_6315172 proquest_miscellaneous_2179338725 pubmed_primary_30631309 crossref_citationtrail_10_3389_fmicb_2018_03052 crossref_primary_10_3389_fmicb_2018_03052 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2018-12-12 |
| PublicationDateYYYYMMDD | 2018-12-12 |
| PublicationDate_xml | – month: 12 year: 2018 text: 2018-12-12 day: 12 |
| PublicationDecade | 2010 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland |
| PublicationTitle | Frontiers in microbiology |
| PublicationTitleAlternate | Front Microbiol |
| PublicationYear | 2018 |
| Publisher | Frontiers Media S.A |
| Publisher_xml | – name: Frontiers Media S.A |
| References | Liu (B35) 2013; 195 Ramirez-Mata (B45) 2018; 215 Smits (B61) 2010; 23 Daims (B13) 2006; 8 Nakai (B40) 2001; 19 Remus-Emsermann (B48); 1 Lin (B34) 2012; 93 Suarez (B63) 1997; 196 Million-Weaver (B38) 2012; 834 Reznikoff (B52) 2008; 42 Tolker-Nielsen (B67) 2000; 40 Winterberg (B71) 2005; 71 McKenzie (B36) 2006; 6 Parks (B42) 2007; 189 Ledermann (B33) 2015; 28 Rodriguez (B53) 2017; 8 Bajar (B4) 2016; 16 Remus-Emsermann (B51) 2018; 218 Zimmermann (B73) 2005 de Lorenzo (B15) 1990; 172 Remus-Emsermann (B46); 363 Klümper (B26) 2014; 5 Enne (B17) 2005; 56 Zengerer (B72) 2018; 9 Christen (B11) 2011; 7 Whitaker (B70) 2017; 169 Innerebner (B24) 2011; 77 Tecon (B66) 2012; 14 Lambertsen (B31) 2004; 6 Peredo (B43) 2017; 8 Stewart (B62) 2001; 358 Kremers (B27) 2007; 46 Fernandes (B19) 2001; 147 Bai (B3) 2015; 528 Schmidt (B58) 2018; 6 Schmid (B57) 2018; 46 Gallagher (B20) 2011; 2 Schada von Borzyskowski (B55) 2015; 4 Remus-Emsermann (B47); 11 Andersson (B2) 2011; 35 Choi (B10) 2006; 1 Benoit (B6) 2016; 11 Chu (B12) 2014; 11 Diard (B16) 2013; 494 Andersen (B1) 1998; 64 Schindelin (B56) 2012; 9 Summers (B65) 1991; 9 Kroupitski (B28) 2009; 75 Valm (B68) 2012; 35 Das (B14) 2005; 63 Goedhart (B22) 2012; 3 Miller (B37) 2000; 13 Lambert (B30) 2018 Feil (B18) 2005; 102 Carroll (B9) 2011; 188 Vidal (B69) 2009; 4 Barbier (B5) 2016; 11 Remus-Emsermann (B49); 5 Gibson (B21) 2009; 6 Monier (B39) 2005; 71 Nilsson (B41) 2014; 3 Lau (B32) 2013; 70 Subach (B64) 2011; 6 Remus-Emsermann (B50) 2014; 16 Simon (B59) 1983; 1 Bloemberg (B8) 2000; 13 Bindels (B7) 2017; 14 Jinek (B25) 2012; 337 Peters (B44) 2015 Harder (B23) 1973; 78 Smith (B60) 1998; 44 Lagendijk (B29) 2010; 305 Sambrook (B54) 1989 |
| References_xml | – volume: 834 start-page: 33 year: 2012 ident: B38 article-title: Quantifying plasmid copy number to investigate plasmid dosage effects associated with directed protein evolution. publication-title: Methods Mol. Biol. doi: 10.1007/978-1-61779-483-4_3 – volume: 169 start-page: e12 year: 2017 ident: B70 article-title: Tunable expression tools enable single-cell strain distinction in the gut microbiome. publication-title: Cell doi: 10.1016/j.cell.2017.03.041 – volume: 35 start-page: 496 year: 2012 ident: B68 article-title: CLASI-FISH: principles of combinatorial labeling and spectral imaging. publication-title: Syst. Appl. Microbiol. doi: 10.1016/j.syapm.2012.03.004 – volume: 147 start-page: 2529 year: 2001 ident: B19 article-title: Construction of Rhodococcus random mutagenesis libraries using Tn5 transposition complexes. publication-title: Microbiology doi: 10.1099/00221287-147-9-2529 – volume: 75 start-page: 6076 year: 2009 ident: B28 article-title: Internalization of Salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.01084-09 – volume: 5 start-page: 387 ident: B49 article-title: Single-cell versus population-level reproductive success of bacterial immigrants to pre-colonized leaf surfaces. publication-title: Environ. Microbiol. Rep. doi: 10.1111/1758-2229.12040 – volume: 2 start-page: 315 year: 2011 ident: B20 article-title: Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq. publication-title: mBio doi: 10.1128/mBio.00315-10 – start-page: 647 year: 2015 ident: B44 doi: 10.1128/microbiolspec.MDNA3-0010-2014 – volume: 13 start-page: 1243 year: 2000 ident: B37 article-title: Improved gfp and inaZ broad-host-range promoter-probe vectors. publication-title: Mol. Plant Microbe Interact. doi: 10.1094/MPMI.2000.13.11.1243 – volume: 195 start-page: 629 year: 2013 ident: B35 article-title: Use of a mariner-based transposon mutagenesis system to isolate Clostridium perfringens mutants deficient in gliding motility. publication-title: J. Bacteriol. doi: 10.1128/JB.01288-12 – volume: 93 start-page: 1185 year: 2012 ident: B34 article-title: The actinobacterium Microbacterium sp. 16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots, and fixes N2 associated with micropropagated sugarcane plants. publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-011-3618-3 – volume: 6 year: 2018 ident: B58 article-title: Recognizing patterns: spatial analysis of observed microbial colonization on root surfaces. publication-title: Front. Environ. Sci. Eng. doi: 10.3389/fenvs.2018.00061 – volume: 218 start-page: 1327 year: 2018 ident: B51 article-title: Phyllosphere microbiology: at the interface between microbial individuals and the plant host. publication-title: New Phytol. doi: 10.1111/nph.15054 – volume: 6 year: 2006 ident: B36 article-title: Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. publication-title: BMC Microbiol. doi: 10.1186/1471-2180-6-39 – volume: 172 start-page: 6568 year: 1990 ident: B15 article-title: Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. publication-title: J. Bacteriol. doi: 10.1128/jb.172.11.6568-6572.1990 – volume: 5 year: 2014 ident: B26 article-title: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2014.00730 – volume: 3 start-page: 333 year: 2014 ident: B41 article-title: A mariner transposon vector adapted for mutagenesis in oral streptococci. publication-title: Microbiologyopen doi: 10.1002/mbo3.171 – volume: 63 start-page: 89 year: 2005 ident: B14 article-title: An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites. publication-title: J. Microbiol. Methods doi: 10.1016/j.mimet.2005.02.011 – volume: 8 year: 2017 ident: B43 article-title: Leaf-FISH: microscale imaging of bacterial taxa on phyllosphere. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2017.02669 – volume: 363 ident: B46 article-title: MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae. publication-title: FEMS Microbiol. Lett. doi: 10.1093/femsle/fnw178 – volume: 56 start-page: 544 year: 2005 ident: B17 article-title: Assessment of the fitness impacts on Escherichia coli of acquisition of antibiotic resistance genes encoded by different types of genetic element. publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dki255 – volume: 14 start-page: 53 year: 2017 ident: B7 article-title: mScarlet: a bright monomeric red fluorescent protein for cellular imaging. publication-title: Nat. Methods doi: 10.1038/nmeth.4074 – volume: 16 year: 2016 ident: B4 article-title: Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. publication-title: Sci. Rep. doi: 10.1038/srep20889 – volume: 71 start-page: 451 year: 2005 ident: B71 article-title: Phenotypic screening of Escherichia coli K-12 Tn5 insertion libraries, using whole-genome oligonucleotide microarrays. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.71.1.451-459.2005 – volume: 8 start-page: 200 year: 2006 ident: B13 article-title: daime, a novel image analysis program for microbial ecology and biofilm research. publication-title: Environ. Microbiol. doi: 10.1111/j.1462-2920.2005.00880.x – volume: 4 start-page: 430 year: 2015 ident: B55 article-title: A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in Methylobacterium extorquens AM1 and other alphaproteobacteria. publication-title: ACS Synth. Biol. doi: 10.1021/sb500221v – volume: 7 year: 2011 ident: B11 article-title: The essential genome of a bacterium. publication-title: Mol. Syst. Biol. doi: 10.1038/msb.2011.58 – start-page: 245 year: 2005 ident: B73 article-title: “Spectral imaging and linear unmixing in light microscopy,” in publication-title: Microscopy Techniques Advances in Biochemical Engineering/Biotechnology doi: 10.1007/b102216 – volume: 102 start-page: 11064 year: 2005 ident: B18 article-title: Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000. publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.0504930102 – volume: 215 start-page: 155 year: 2018 ident: B45 article-title: Versatile use of Azospirillum brasilense strains tagged with egfp and mCherry genes for the visualization of biofilms associated with wheat roots. publication-title: Microbiol. Res. doi: 10.1016/j.micres.2018.07.007 – volume: 46 start-page: 3775 year: 2007 ident: B27 article-title: Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. publication-title: Biochemistry doi: 10.1021/bi0622874 – year: 1989 ident: B54 publication-title: Molecular Cloning: A Laboratory Manual – volume: 528 start-page: 364 year: 2015 ident: B3 article-title: Functional overlap of the Arabidopsis leaf and root microbiota. publication-title: Nature doi: 10.1038/nature16192 – volume: 6 start-page: 343 year: 2009 ident: B21 article-title: Enzymatic assembly of DNA molecules up to several hundred kilobases. publication-title: Nat. Methods doi: 10.1038/nmeth.1318 – volume: 77 start-page: 3202 year: 2011 ident: B24 article-title: Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.00133-11 – volume: 46 start-page: 8953 year: 2018 ident: B57 article-title: Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats. publication-title: Nucleic Acids Res. doi: 10.1093/nar/gky726 – volume: 35 start-page: 901 year: 2011 ident: B2 article-title: Persistence of antibiotic resistance in bacterial populations. publication-title: FEMS Microbiol. Rev. doi: 10.1111/j.1574-6976.2011.00289.x – volume: 28 start-page: 959 year: 2015 ident: B33 article-title: Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoefficiens to analyze host-plant infection and colonization. publication-title: Mol. Plant Microbe Interact. doi: 10.1094/MPMI-03-15-0054-TA – volume: 23 start-page: 384 year: 2010 ident: B61 article-title: Complete genome sequence of the fire blight pathogen Erwinia amylovora CFBP 1430 and comparison to other Erwinia spp. publication-title: Mol. Plant Microbe Interact. doi: 10.1094/MPMI-23-4-0384 – volume: 358 start-page: 135 year: 2001 ident: B62 article-title: Antibiotic resistance of bacteria in biofilms. publication-title: Lancet doi: 10.1016/S0140-6736(01)05321-1 – volume: 8 year: 2017 ident: B53 article-title: Construction of stable fluorescent reporter plasmids for use in Staphylococcus aureus. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2017.02491 – volume: 6 year: 2011 ident: B64 article-title: An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. publication-title: PLoS One doi: 10.1371/journal.pone.0028674 – volume: 337 start-page: 816 year: 2012 ident: B25 article-title: A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. publication-title: Science doi: 10.1126/science.1225829 – volume: 42 start-page: 269 year: 2008 ident: B52 article-title: Transposon Tn5. publication-title: Annu. Rev. Genet. doi: 10.1146/annurev.genet.42.110807.091656 – volume: 11 start-page: 572 year: 2014 ident: B12 article-title: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. publication-title: Nat. Methods doi: 10.1038/nmeth.2888 – volume: 1 year: 1983 ident: B59 article-title: A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. publication-title: Biotechnology doi: 10.1038/nbt1183-784 – volume: 4 year: 2009 ident: B69 article-title: Use of an EZ-Tn5-based random mutagenesis system to identify a novel toxin regulatory locus in Clostridium perfringens strain 13. publication-title: PLoS One doi: 10.1371/journal.pone.0006232 – volume: 9 year: 2018 ident: B72 article-title: Pseudomonas orientalis F9: a potent antagonist against phytopathogens with phytotoxic effect in the apple flower. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2018.00145 – volume: 11 year: 2016 ident: B5 article-title: Rainbow vectors for broad-range bacterial fluorescence labeling. publication-title: PLoS One doi: 10.1371/journal.pone.0146827 – volume: 11 year: 2016 ident: B6 article-title: Seamless insert-plasmid assembly at high efficiency and low cost. publication-title: PLoS One doi: 10.1371/journal.pone.0153158 – volume: 13 start-page: 1170 year: 2000 ident: B8 article-title: Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different autofluorescent proteins in the rhizosphere: new perspectives for studying microbial communities. publication-title: Mol. Plant Microbe Interact. doi: 10.1094/MPMI.2000.13.11.1170 – volume: 40 start-page: 75 year: 2000 ident: B67 article-title: Spatial organization of microbial biofilm communities. publication-title: Microb. Ecol. doi: 10.1007/s002480000057 – volume: 9 start-page: 273 year: 1991 ident: B65 article-title: The kinetics of plasmid loss. publication-title: Trends Biotechnol. doi: 10.1016/0167-7799(91)90089-Z – volume: 188 start-page: 773 year: 2011 ident: B9 article-title: Genome engineering with zinc-finger nucleases. publication-title: Genetics doi: 10.1534/genetics.111.131433 – volume: 44 start-page: 351 year: 1998 ident: B60 article-title: Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size. publication-title: Can. J. Microbiol. doi: 10.1139/w98-020 – volume: 14 start-page: 1325 year: 2012 ident: B66 article-title: The mechanics of bacterial cluster formation on plant leaf surfaces as revealed by bioreporter technology. publication-title: Environ. Microbiol. doi: 10.1111/j.1462-2920.2012.02715.x – volume: 1 start-page: 153 year: 2006 ident: B10 article-title: mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. publication-title: Nat. Protoc. doi: 10.1038/nprot.2006.24 – volume: 196 start-page: 69 year: 1997 ident: B63 article-title: Green fluorescent protein-based reporter systems for genetic analysis of bacteria including monocopy applications. publication-title: Gene doi: 10.1016/S0378-1119(97)00197-2 – year: 2018 ident: B30 publication-title: Tlambert03/Fpbase: V1.1.0. Zenodo doi: 10.5281/ZENODO.1244328 – volume: 305 start-page: 81 year: 2010 ident: B29 article-title: Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. publication-title: FEMS Microbiol. Lett. doi: 10.1111/j.1574-6968.2010.01916.x – volume: 11 ident: B47 article-title: Complete genome sequence of Pseudomonas citronellolis P3B5, a candidate for microbial phyllo-remediation of hydrocarbon-contaminated sites. publication-title: Stand. Genomic Sci. doi: 10.1186/s40793-016-0190-6 – volume: 19 start-page: 137 year: 2001 ident: B40 article-title: A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. publication-title: Nat. Biotechnol. doi: 10.1038/84397 – volume: 6 start-page: 726 year: 2004 ident: B31 article-title: Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins. publication-title: Environ. Microbiol. doi: 10.1111/j.1462-2920.2004.00605.x – volume: 16 start-page: 2329 year: 2014 ident: B50 article-title: Spatial distribution analyses of natural phyllosphere-colonizing bacteria on Arabidopsis thaliana revealed by fluorescence in situ hybridization. publication-title: Environ. Microbiol. doi: 10.1111/1462-2920.12482 – volume: 9 start-page: 676 year: 2012 ident: B56 article-title: Fiji: an open-source platform for biological-image analysis. publication-title: Nat. Methods doi: 10.1038/nmeth.2019 – volume: 1 ident: B48 article-title: Draft genome sequence of the phyllosphere model bacterium Pantoea agglomerans 299R. publication-title: Genome Announc. doi: 10.1128/genomeA.00036-13 – volume: 78 start-page: 155 year: 1973 ident: B23 article-title: Methanol assimilation by Hyphomicrobium sp. publication-title: Microbiology – volume: 71 start-page: 5484 year: 2005 ident: B39 article-title: Spatial organization of dual-species bacterial aggregates on leaf surfaces. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.71.9.5484-5493.2005 – volume: 3 year: 2012 ident: B22 article-title: Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. publication-title: Nat. Commun. doi: 10.1038/ncomms1738 – volume: 70 start-page: 353 year: 2013 ident: B32 article-title: New quantitative methods for measuring plasmid loss rates reveal unexpected stability. publication-title: Plasmid doi: 10.1016/j.plasmid.2013.07.007 – volume: 64 start-page: 2240 year: 1998 ident: B1 article-title: New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.64.6.2240-2246.1998 – volume: 189 start-page: 2170 year: 2007 ident: B42 article-title: Transposon Tn7 is widespread in diverse bacteria and forms genomic islands. publication-title: J. Bacteriol. doi: 10.1128/JB.01536-06 – volume: 494 start-page: 353 year: 2013 ident: B16 article-title: Stabilization of cooperative virulence by the expression of an avirulent phenotype. publication-title: Nature doi: 10.1038/nature11913 |
| SSID | ssj0000402000 |
| Score | 2.4514298 |
| Snippet | Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation,... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 3052 |
| SubjectTerms | fluorescent labeling fluorophore Microbiology tagging Tn5 Tn7 transposon |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LatwwFBUhUMgm9Jm6L1ToJgs3tmyN5GVSMkwLLSEkkJ3QkwxM5ZCZgXRX-gv9w35J75U8w0wp6aY7v4SFzpV0jnV9RMg72RrDXBXKVnv8dCNdabxrS5jqmto1QJo1_uD8-ctoctl-uuJXG1t9YU5YtgfODXfEK3TIGsmOswCTfadbW4daey8tsBeXhE_VVRtiKo3BKIuqKq9LggrrAKapNZjKJd9jjLOteSjZ9f-NY_6ZKrkx94wfkv2BNNLjXNlHZMfHx-RB3kby2xPyAx1uk_UqPcnmy5r--v6THlMQ2drRST9flOf4FwE9A7L8deqojo6mUv0cSs7ox4ir8oARvej7menvKJBZOp4t-9vs90TP0NBhGunp3ZA6Gymcrd73lFyOTy8-TMphb4XSciYXpQSQ4Mh2TgZXO25N62pmRyJIV9VBilALOXLGCGMkqChtOcJm0X0n8NA1z8hu7KN_TqhthHGtQSYAatEE46TnrXAswHUtqoIcrVpa2cF4HPe_mCkQIIiNStgoxEYlbApyuC5xk0037nn2BMFbP4d22ekCBJEagkj9K4gK8nYFvYLuhWsmOvp-OVcMB7BGCsYLcpBDYf0qUFsNUICuIGIrSLbqsn0nTq-ThTcU5EAdX_yPyr8ke9gcmGNTs1dkd3G79K-BKS3Mm9QpfgN_1RPE priority: 102 providerName: Directory of Open Access Journals |
| Title | Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30631309 https://www.proquest.com/docview/2179338725 https://pubmed.ncbi.nlm.nih.gov/PMC6315172 https://doaj.org/article/50681268952f4569a4c1f1aee8c013d1 |
| Volume | 9 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bi9QwFA6youyLeLdelgi--NC1aZtJ-iCyKzuOgrLIDsxbyKVZB2qzOxeYfRP_gv_QX-I5aWd0ZPDBl9LbISXfSfKd5uQLIS9kaUzuMp-WusZfN9KlpnZlCkNdwVwBpFnjAuePnwajcflhwie_l0f3FTjfGdrhflLjWXO4urx6Aw3-NUacMN4CAlNrMEtLHqL7Qod8Pc4WYSJfT_Zjv4yhUpZ1c5U7DffJTWDQBZhWW8NUVPPfRUH_zqT8Y2ga3ia3ek5JjzonuEOu1e1dcqPbZfLqHvmOArhRmZUed9rMmv789oMeUYjBtaOjMF-kn3GRAT0FLv116qhuHY1WYQ6WDX3f4qQ9QEjPQmhMWFHgunTYLMOsk4Oip6j3MG3pyarPrG0pXK3Lu0_Gw5Ozt6O033ohtTyXi1QChnBmKye9Y45bUzqW24Hw0mXMS-GZkANnjDBGQpClLUdULYrzeO6r4gHZa0NbPyLUFsK40iBRgGDSeONkzUvhcg_3tcgS8mpd08r2uuS4PUajID5BmFSESSFMKsKUkJcbi4tOk-Mf7x4jeJv3UE073gizc9U3TsUzVGEbyIrnHghlpUvLPNN1LS0wZMcS8nwNvYLWh1Mquq3Dcq5y7N8KKXKekIedK2yKWrtSQsSWk2x9y_aTdvolKnyDIQdm-fi_LZ-QfawDzLth-VOyt5gt62fAnhbmIP51gOO7CTuIDeQXcT4e_g |
| linkProvider | Scholars Portal |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Chromatic+Bacteria+%E2%80%93+A+Broad+Host-Range+Plasmid+and+Chromosomal+Insertion+Toolbox+for+Fluorescent+Protein+Expression+in+Bacteria&rft.jtitle=Frontiers+in+microbiology&rft.au=Schlechter%2C+Rudolf+O.&rft.au=Jun%2C+Hyunwoo&rft.au=Bernach%2C+Micha%C5%82&rft.au=Oso%2C+Simisola&rft.date=2018-12-12&rft.pub=Frontiers+Media+S.A&rft.eissn=1664-302X&rft.volume=9&rft_id=info:doi/10.3389%2Ffmicb.2018.03052&rft_id=info%3Apmid%2F30631309&rft.externalDocID=PMC6315172 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-302X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-302X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-302X&client=summon |