Longitudinal qPCR Study of the Dynamics of L. crispatus, L. iners, A. vaginae, (Sialidase Positive) G. vaginalis, and P. bivia in the Vagina
To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles. Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, th...
Saved in:
| Published in | PloS one Vol. 7; no. 9; p. e45281 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
21.09.2012
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1932-6203 1932-6203 |
| DOI | 10.1371/journal.pone.0045281 |
Cover
| Abstract | To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles.
Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar.
Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7-9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H(2)O(2)-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4-8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF.
This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. |
|---|---|
| AbstractList | To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles.BACKGROUNDTo obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles.Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar.METHODSVaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar.Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7-9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H(2)O(2)-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4-8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF.RESULTSWomen could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7-9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H(2)O(2)-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4-8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF.This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies.CONCLUSIONSThis qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. BACKGROUND: To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles. METHODS: Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar. RESULTS: Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7-9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H(2)O(2)-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4-8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF. CONCLUSIONS: This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles. Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar. Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7-9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H(2)O(2)-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4-8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF. This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. Background To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles. Methods Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae , Lactobacillus crispatus , L. iners , (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar. Results Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7–9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H 2 O 2 -production. L. iner s was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4–8 cells/ml) was mostly present in grade III vaginal microflora. L. iners , G. vaginalis , A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF. Conclusions This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. Background To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles. Methods Vaginal swabs were obtained daily from 17 non-pregnant, menarchal volunteers. For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar. Results Women could be divided in 9 subjects with predominantly normal VMF (grades Ia, Ib and Iab, group N) and 8 with predominantly disturbed VMF (grades I-like, II, III and IV, group D). VMF was variable between women, but overall stable for most of the women. Menses were the strongest disturbing factor of the VMF. L. crispatus was present at log7–9 cells/ml in grade Ia, Iab and II VMF, but concentrations declined 100-fold during menses. L. crispatus below log7 cells/ml corresponded with poor H2O2-production. L. iners was present at log 10 cells/ml in grade Ib, II and III VMF. Sialidase negative G. vaginalis strains (average log5 cells/ml) were detected in grade I, I-like and IV VMF. In grade II VMF, predominantly a mixture of both sialidase negative and positive G. vaginalis strains (average log9 cells/ml) were present, and predominantly sialidase positive strains in grade III VMF. The presence of A. vaginae (average log9 cells/ml) coincided with grade II and III VMF. P. bivia (log4–8 cells/ml) was mostly present in grade III vaginal microflora. L. iners, G. vaginalis, A. vaginae and P. bivia all increased around menses for group N women, and as such L. iners was considered a member of disturbed VMF. Conclusions This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies. |
| Author | Lopes dos Santos Santiago, Guido Temmerman, Marleen Decat, Ellen Tency, Inge Verstraelen, Hans Trog, Marijke Vancoillie, Leen Vaneechoutte, Mario Verhelst, Rita Cools, Piet |
| AuthorAffiliation | 4 Faculty of Health Care, University College Ghent, Ghent, Belgium 3 AIDS Reference Laboratory, Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University/Ghent University Hospital, Ghent, Belgium 1 Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium 2 Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium University of Iowa Carver College of Medicine, United States of America |
| AuthorAffiliation_xml | – name: 4 Faculty of Health Care, University College Ghent, Ghent, Belgium – name: 3 AIDS Reference Laboratory, Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University/Ghent University Hospital, Ghent, Belgium – name: 2 Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium – name: University of Iowa Carver College of Medicine, United States of America – name: 1 Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium |
| Author_xml | – sequence: 1 givenname: Guido surname: Lopes dos Santos Santiago fullname: Lopes dos Santos Santiago, Guido – sequence: 2 givenname: Inge surname: Tency fullname: Tency, Inge – sequence: 3 givenname: Hans surname: Verstraelen fullname: Verstraelen, Hans – sequence: 4 givenname: Rita surname: Verhelst fullname: Verhelst, Rita – sequence: 5 givenname: Marijke surname: Trog fullname: Trog, Marijke – sequence: 6 givenname: Marleen surname: Temmerman fullname: Temmerman, Marleen – sequence: 7 givenname: Leen surname: Vancoillie fullname: Vancoillie, Leen – sequence: 8 givenname: Ellen surname: Decat fullname: Decat, Ellen – sequence: 9 givenname: Piet surname: Cools fullname: Cools, Piet – sequence: 10 givenname: Mario surname: Vaneechoutte fullname: Vaneechoutte, Mario |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23028904$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kltrFDEUgAep2Iv-A9GALxW6Y24zyfgglFVrYcHFqq_hJJPZZpmdbJOZhf0P_mizt9IW8Sk5yXe-nBPOaXbU-c5m2WuCc8IE-TD3Q-igzZfpOMeYF1SSZ9kJqRgdlRSzowf74-w0xjnGBZNl-SI7pgxTWWF-kv2Z-G7m-qF2yYXupuMf6CZFa-Qb1N9a9HndwcKZuIknOTLBxSX0Q7zYRK6zIe0uc7SCWRLYC3R-46B1NUSLpj663q3se3R1AFqXcOhqNM2RdisHSbF95vf2-mX2vIE22lf79Sz79fXLz_G30eT71fX4cjIyBS37kdBaEi25gEIAEEsI4dSUVGNbCUbrsqJWVppXoEFrTmpa6IbjUkgwlNWYnWVvd95l66Paf2RUhNGyKLBkRSKud0TtYa6WwS0grJUHp7YHPswUhN6Z1ioqrGGiEjWvCW-wqKwkDYApG84LzXVyfdq_NuiFrY3t-gDtI-njm87dqplfKca5TAUlwfleEPzdYGOvFi4a27bQWT-kurGkqT0pRELfPUH_3d2bhxXdl3IYiwTwHWCCjzHY5h4hWG2m76BVm-lT--lLaR-fpBnXQ-_8pi_X_j_5L0BR4ZM |
| CitedBy_id | crossref_primary_10_1111_aji_13435 crossref_primary_10_1371_journal_pone_0300510 crossref_primary_10_3389_fcimb_2020_00305 crossref_primary_10_4103_2249_4863_214982 crossref_primary_10_3389_fcimb_2019_00452 crossref_primary_10_1007_s10482_017_0992_6 crossref_primary_10_1128_mbio_00691_24 crossref_primary_10_1371_journal_pone_0105998 crossref_primary_10_3389_fendo_2018_00568 crossref_primary_10_1371_journal_pone_0060670 crossref_primary_10_1128_AEM_01594_20 crossref_primary_10_1128_AEM_02200_17 crossref_primary_10_1128_msphere_00593_20 crossref_primary_10_3389_fimmu_2018_00376 crossref_primary_10_1186_s40168_021_01198_4 crossref_primary_10_1371_journal_pone_0166794 crossref_primary_10_1371_journal_pone_0171856 crossref_primary_10_1097_QAI_0000000000000866 crossref_primary_10_1007_s12602_014_9183_1 crossref_primary_10_18705_2782_3806_2022_2_6_78_90 crossref_primary_10_1080_1040841X_2022_2049696 crossref_primary_10_3390_diagnostics10110879 crossref_primary_10_3389_fcimb_2023_1198113 crossref_primary_10_1016_j_micpath_2020_104029 crossref_primary_10_1371_journal_pone_0153441 crossref_primary_10_1007_s00129_014_3379_z crossref_primary_10_1016_j_resmic_2017_07_004 crossref_primary_10_3389_fimmu_2022_928475 crossref_primary_10_1097_EDE_0000000000001463 crossref_primary_10_1371_journal_pone_0082599 crossref_primary_10_1016_j_rbmo_2022_06_008 crossref_primary_10_3389_fphys_2015_00164 crossref_primary_10_1097_LGT_0000000000000643 crossref_primary_10_1097_MRM_0000000000000186 crossref_primary_10_1186_s12879_015_0825_z crossref_primary_10_3389_fmicb_2017_00564 crossref_primary_10_1038_s41467_020_19382_9 crossref_primary_10_1097_QAD_0b013e32836290b6 crossref_primary_10_3389_fgwh_2022_810673 crossref_primary_10_1038_srep05136 crossref_primary_10_1016_j_ajog_2018_10_014 crossref_primary_10_1016_j_resmic_2017_09_003 crossref_primary_10_3389_fphys_2015_00081 crossref_primary_10_1093_infdis_jiz342 crossref_primary_10_1093_infdis_jiaa676 crossref_primary_10_24075_brsmu_2025_007 crossref_primary_10_3920_BM2015_0174 crossref_primary_10_3389_fcimb_2020_00083 crossref_primary_10_1016_j_resmic_2017_08_001 crossref_primary_10_1186_s12879_024_09455_1 crossref_primary_10_1016_j_anaerobe_2013_07_007 crossref_primary_10_1016_j_fertnstert_2015_10_010 crossref_primary_10_1186_s12866_019_1545_0 crossref_primary_10_3389_fcimb_2023_1059339 crossref_primary_10_1016_j_fertnstert_2018_06_036 crossref_primary_10_1016_j_micpath_2021_104780 crossref_primary_10_1038_s41522_023_00454_9 crossref_primary_10_1093_cid_ciw638 crossref_primary_10_3389_fcimb_2021_676114 crossref_primary_10_1097_GME_0000000000002358 crossref_primary_10_1371_journal_pone_0059539 crossref_primary_10_3389_fcimb_2021_792787 crossref_primary_10_1136_sextrans_2018_053769 crossref_primary_10_1097_QAD_0000000000001768 crossref_primary_10_1177_2377960816662286 crossref_primary_10_3389_fimmu_2019_02823 crossref_primary_10_1128_msystems_00689_22 crossref_primary_10_1016_j_tim_2016_11_007 crossref_primary_10_3389_fmed_2018_00181 crossref_primary_10_1038_ismej_2014_26 crossref_primary_10_1007_s00414_021_02525_y crossref_primary_10_1016_j_ajog_2020_08_034 crossref_primary_10_1128_mBio_00097_15 crossref_primary_10_1088_1742_6596_1294_6_062072 crossref_primary_10_1111_aji_12979 |
| ContentType | Journal Article |
| Copyright | Lopes dos Santos Santiago et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2012 Lopes dos Santos Santiago et al 2012 Lopes dos Santos Santiago et al |
| Copyright_xml | – notice: Lopes dos Santos Santiago et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2012 Lopes dos Santos Santiago et al 2012 Lopes dos Santos Santiago et al |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0045281 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection ProQuest Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE Agricultural Science Database |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Medicine Biology |
| DocumentTitleAlternate | Longitudinal qPCR Study of 5 Key Vaginal Species |
| EISSN | 1932-6203 |
| ExternalDocumentID | 1326550835 oai_doaj_org_article_27ec3797d4d14f079e81faac6f445b4b PMC3448655 2943665971 23028904 10_1371_journal_pone_0045281 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | Belgium |
| GeographicLocations_xml | – name: Belgium |
| GroupedDBID | --- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO PTHSS PYCSY RIG RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM BBORY CGR CUY CVF ECM EIF NPM PV9 RZL 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PJZUB PKEHL PPXIY PQEST PQGLB PQUKI PRINS RC3 7X8 ESTFP PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c526t-7bb81b847a57aa1e11142c62b0e9732d692e89b49ababb41d25bf40678ac23d03 |
| IEDL.DBID | M48 |
| ISSN | 1932-6203 |
| IngestDate | Fri Nov 26 17:12:33 EST 2021 Wed Aug 27 01:25:51 EDT 2025 Thu Aug 21 13:58:47 EDT 2025 Mon Sep 08 10:20:21 EDT 2025 Fri Jul 25 10:46:04 EDT 2025 Fri May 16 01:56:55 EDT 2025 Tue Jul 01 04:15:23 EDT 2025 Thu Apr 24 23:00:51 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c526t-7bb81b847a57aa1e11142c62b0e9732d692e89b49ababb41d25bf40678ac23d03 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: HV RV M. Trog M. Temmerman ED MV GL. Performed the experiments: HV RV M. Trog LV GL. Analyzed the data: HV RV LV PC MV GL. Wrote the paper: IT HV RV M. Trog M. Temmerman LV ED PC MV GL. Competing Interests: The authors have declared that no competing interests exist. |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.1371/journal.pone.0045281 |
| PMID | 23028904 |
| PQID | 1326550835 |
| PQPubID | 1436336 |
| ParticipantIDs | plos_journals_1326550835 doaj_primary_oai_doaj_org_article_27ec3797d4d14f079e81faac6f445b4b pubmedcentral_primary_oai_pubmedcentral_nih_gov_3448655 proquest_miscellaneous_1082406877 proquest_journals_1326550835 pubmed_primary_23028904 crossref_primary_10_1371_journal_pone_0045281 crossref_citationtrail_10_1371_journal_pone_0045281 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2012-09-21 |
| PublicationDateYYYYMMDD | 2012-09-21 |
| PublicationDate_xml | – month: 09 year: 2012 text: 2012-09-21 day: 21 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2012 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | 16225680 - BMC Microbiol. 2005;5:61 17215167 - Best Pract Res Clin Obstet Gynaecol. 2007 Jun;21(3):347-54 17714681 - Am J Obstet Gynecol. 2008 Jan;198(1):132.e1-7 21059957 - Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4688-95 9218863 - Can J Gastroenterol. 1997 May-Jun;11(4):361-6 21444061 - Am J Obstet Gynecol. 2011 May;204(5):450.e1-7 15995952 - J Infect Dis. 2005 Aug 1;192(3):394-8 15102329 - BMC Microbiol. 2004 Apr 21;4:16 3262199 - N Engl J Med. 1988 Oct 13;319(15):972-8 9155551 - Genitourin Med. 1997 Feb;73(1):23-8 9259496 - Int J STD AIDS. 1997 Aug;8(8):489-94 20482854 - BMC Infect Dis. 2010;10:120 1551983 - J Clin Microbiol. 1992 Mar;30(3):663-6 20305015 - J Clin Microbiol. 2010 May;48(5):1812-9 15018635 - BMC Infect Dis. 2004 Feb 13;4:5 1738516 - Obstet Gynecol. 1992 Mar;79(3):369-73 20419168 - PLoS One. 2010;5(4):e10197 20711427 - PLoS One. 2010;5(8):e12078 21771337 - BMC Infect Dis. 2011;11:200 17999885 - Curr Infect Dis Rep. 2007 Nov;9(6):485-500 11368254 - Infect Dis Obstet Gynecol. 2001;9(1):17-22 19490622 - BMC Microbiol. 2009;9:116 10515826 - J Infect Dis. 1999 Nov;180(5):1632-6 10852812 - Clin Infect Dis. 2000 Jun;30(6):901-7 16267321 - N Engl J Med. 2005 Nov 3;353(18):1899-911 21068695 - Sex Transm Dis. 2010 Dec;37(12):732-44 21513795 - Res Microbiol. 2011 Jun;162(5):506-13 22140538 - PLoS One. 2011;6(11):e28180 1647428 - J Infect Dis. 1991 Jul;164(1):94-100 16272477 - J Clin Microbiol. 2005 Nov;43(11):5504-8 16260520 - Obstet Gynecol. 2005 Nov;106(5 Pt 1):1013-23 18762999 - Eur J Clin Microbiol Infect Dis. 2009 Mar;28(3):281-5 20534435 - Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4680-7 9203662 - J Infect Dis. 1997 Feb;175(2):406-13 18093311 - BMC Microbiol. 2007;7:115 7851968 - Indian J Med Res. 1994 Oct;100:172-6 19733660 - Eur J Pharm Biopharm. 2009 Nov;73(3):399-403 21051845 - Gynecol Obstet Invest. 2010;70(4):256-63 18510764 - BMC Infect Dis. 2008;8:73 8166188 - Am J Obstet Gynecol. 1994 Apr;170(4):1048-59; discussion 1059-60 15071062 - J Clin Microbiol. 2004 Apr;42(4):1829-31 18475388 - Infect Dis Obstet Gynecol. 1994;2(4):179-83 12843073 - J Clin Microbiol. 2003 Jul;41(7):3260-4 9310218 - Int J STD AIDS. 1997 Oct;8(10):603-8 20660593 - Sex Transm Infect. 2010 Aug;86(4):297-302 19948869 - Appl Environ Microbiol. 2010 Jan;76(2):400-5 22553250 - Sci Transl Med. 2012 May 2;4(132):132ra52 19828036 - BMC Infect Dis. 2009;9:167 18513147 - Clin Infect Dis. 2008 Jul 1;47(1):33-43 15717268 - J Infect Dis. 2005 Mar 15;191(6):924-9 11714935 - Sex Transm Infect. 2001 Dec;77(6):402-8 21075899 - Appl Environ Microbiol. 2011 Jan;77(1):378-81 2915019 - J Clin Microbiol. 1989 Feb;27(2):251-6 10558952 - J Infect Dis. 1999 Dec;180(6):1950-6 17989585 - Sex Transm Dis. 2008 Jan;35(1):78-83 15800083 - Sex Transm Infect. 2005 Apr;81(2):100-2 15695992 - Am J Obstet Gynecol. 2005 Feb;192(2):489-96 |
| References_xml | – reference: 20534435 - Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4680-7 – reference: 19733660 - Eur J Pharm Biopharm. 2009 Nov;73(3):399-403 – reference: 22140538 - PLoS One. 2011;6(11):e28180 – reference: 9259496 - Int J STD AIDS. 1997 Aug;8(8):489-94 – reference: 17714681 - Am J Obstet Gynecol. 2008 Jan;198(1):132.e1-7 – reference: 16272477 - J Clin Microbiol. 2005 Nov;43(11):5504-8 – reference: 11368254 - Infect Dis Obstet Gynecol. 2001;9(1):17-22 – reference: 1551983 - J Clin Microbiol. 1992 Mar;30(3):663-6 – reference: 22553250 - Sci Transl Med. 2012 May 2;4(132):132ra52 – reference: 17989585 - Sex Transm Dis. 2008 Jan;35(1):78-83 – reference: 18510764 - BMC Infect Dis. 2008;8:73 – reference: 9155551 - Genitourin Med. 1997 Feb;73(1):23-8 – reference: 10558952 - J Infect Dis. 1999 Dec;180(6):1950-6 – reference: 15800083 - Sex Transm Infect. 2005 Apr;81(2):100-2 – reference: 21075899 - Appl Environ Microbiol. 2011 Jan;77(1):378-81 – reference: 15995952 - J Infect Dis. 2005 Aug 1;192(3):394-8 – reference: 21068695 - Sex Transm Dis. 2010 Dec;37(12):732-44 – reference: 15071062 - J Clin Microbiol. 2004 Apr;42(4):1829-31 – reference: 15102329 - BMC Microbiol. 2004 Apr 21;4:16 – reference: 18513147 - Clin Infect Dis. 2008 Jul 1;47(1):33-43 – reference: 21059957 - Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4688-95 – reference: 21444061 - Am J Obstet Gynecol. 2011 May;204(5):450.e1-7 – reference: 20660593 - Sex Transm Infect. 2010 Aug;86(4):297-302 – reference: 15717268 - J Infect Dis. 2005 Mar 15;191(6):924-9 – reference: 16267321 - N Engl J Med. 2005 Nov 3;353(18):1899-911 – reference: 20711427 - PLoS One. 2010;5(8):e12078 – reference: 9218863 - Can J Gastroenterol. 1997 May-Jun;11(4):361-6 – reference: 20305015 - J Clin Microbiol. 2010 May;48(5):1812-9 – reference: 3262199 - N Engl J Med. 1988 Oct 13;319(15):972-8 – reference: 1647428 - J Infect Dis. 1991 Jul;164(1):94-100 – reference: 1738516 - Obstet Gynecol. 1992 Mar;79(3):369-73 – reference: 17215167 - Best Pract Res Clin Obstet Gynaecol. 2007 Jun;21(3):347-54 – reference: 19828036 - BMC Infect Dis. 2009;9:167 – reference: 10852812 - Clin Infect Dis. 2000 Jun;30(6):901-7 – reference: 12843073 - J Clin Microbiol. 2003 Jul;41(7):3260-4 – reference: 7851968 - Indian J Med Res. 1994 Oct;100:172-6 – reference: 11714935 - Sex Transm Infect. 2001 Dec;77(6):402-8 – reference: 17999885 - Curr Infect Dis Rep. 2007 Nov;9(6):485-500 – reference: 9203662 - J Infect Dis. 1997 Feb;175(2):406-13 – reference: 18475388 - Infect Dis Obstet Gynecol. 1994;2(4):179-83 – reference: 15018635 - BMC Infect Dis. 2004 Feb 13;4:5 – reference: 20419168 - PLoS One. 2010;5(4):e10197 – reference: 19490622 - BMC Microbiol. 2009;9:116 – reference: 21513795 - Res Microbiol. 2011 Jun;162(5):506-13 – reference: 15695992 - Am J Obstet Gynecol. 2005 Feb;192(2):489-96 – reference: 2915019 - J Clin Microbiol. 1989 Feb;27(2):251-6 – reference: 18762999 - Eur J Clin Microbiol Infect Dis. 2009 Mar;28(3):281-5 – reference: 18093311 - BMC Microbiol. 2007;7:115 – reference: 19948869 - Appl Environ Microbiol. 2010 Jan;76(2):400-5 – reference: 9310218 - Int J STD AIDS. 1997 Oct;8(10):603-8 – reference: 21051845 - Gynecol Obstet Invest. 2010;70(4):256-63 – reference: 20482854 - BMC Infect Dis. 2010;10:120 – reference: 8166188 - Am J Obstet Gynecol. 1994 Apr;170(4):1048-59; discussion 1059-60 – reference: 16260520 - Obstet Gynecol. 2005 Nov;106(5 Pt 1):1013-23 – reference: 10515826 - J Infect Dis. 1999 Nov;180(5):1632-6 – reference: 16225680 - BMC Microbiol. 2005;5:61 – reference: 21771337 - BMC Infect Dis. 2011;11:200 |
| SSID | ssj0053866 |
| Score | 2.393634 |
| Snippet | To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during... Background To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17... BACKGROUND: To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17... Background To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17... |
| SourceID | plos doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e45281 |
| SubjectTerms | Actinobacteria - genetics Actinobacteria - growth & development Adolescent Adult Agar Atopobium vaginae Bacterial Load Bacteriology Biology Cell culture Correlation analysis Dynamic tests Female Gardnerella vaginalis Gardnerella vaginalis - genetics Gardnerella vaginalis - growth & development Gentian Violet Gynecology Health sciences Humans Hydrogen Hydrogen peroxide Hydrogen Peroxide - metabolism Immunology Laboratories Lactobacillus - genetics Lactobacillus - growth & development Lactobacillus crispatus Lactobacillus iners Longitudinal Studies Medicine Menstrual Cycle Menstruation Microbial Consortia - genetics Microbiota Microflora Microscopy Neuraminidase - metabolism Obstetrics Phenazines Pregnancy Prevotella - genetics Prevotella - growth & development Prevotella bivia Quality Real-Time Polymerase Chain Reaction Stains Studies Vagina Vagina - microbiology Womens health |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEBYlp15K01fcpkWFHhKIHVuWJeuYhqahpCWUBnIzepkaFjutd_dX5EdnxpKXbAnk0ptlyZasGWm-saRvCPmkrQKrbqu05uDpcKdlqsFrSHNbOSmMBdiPvwa-_xDnV_zbdXV9L9QX7gkL9MCh446Z9LaUSjruCt7mUvm6aLW2ouW8Mtzg7JurfHamwhwMo1iIeFCulMVxlEt2M_Q-m1jE62LLEE18_chvuhjGh7Dmv1sm79mgs-fkWQSP9CQ0epc88f0LshuH50gPIof04UtyezFgIKKVw6BX9M_l6U86EcnSoaUA-agLgehHTF9kFKYOmFmWq_EIU3geEK5OMrrGGEbaH9GDEfS0c2DyaNjmtfaH9OtcYNFBcd07eplR0607Da-YqgnZr8jV2Zdfp-dpDLyQ2oqJZSqNATQLdktXUuvCF3jg1gpmco_kPk4o5mtluNJGG8MLxyrTcrR72rLS5eVrstNDV-8RioR2AAE88-D-KuG1Upxr3poaEqVoE1LOUmhsZCXH4BiLZlpqk-CdhL5tUHZNlF1C0s1TN4GV45Hyn1HAm7LIqT3dAE1roqY1j2laQvZQPeYKRqiOiQrp9KuE7M8q83D2x002jF5cktG9H1Yj0rMipKqlTMiboGGbRoJziKvAPCFyS_e2vmI7p-9-TwzhJTjdUPfb__HZ78hTAIkM98iwYp_sLP-u_HsAYkvzYRpzdz9nMZo priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV1Lb9NAEB6VVEJcEC2PGgpaJA6tVKexvd61Dwi1paVCbRUVinqz9uUSKbLTOonEf-BHM-OsA0EV3LLZTdb2vD2z3wC8UyZHq27SMOMY6XCrZKgwaggHJrVSaINuP70aOL8Qp1f883V6vQYX3VkYKqvsdGKrqG1t6B35PkZNIiXs8vTD5DakrlGUXe1aaCjfWsG-byHGHsA6quR00IP1w-OL4WWnm1G6hfAH6BIZ7Xt69Sd15fotungWrRioFsefcE_HdXOfD_p3KeUftunkCTz2TiU7WHDBBqy5ahMenvu0-SZseAlu2I6Hmd59Cj_PaupVNLPUF4vdDo8uGRUV_mB1ydArZB8XveobGp_1GWoXVD7TWbNHIzoyiJ8O-mxObY6U22M7X5CVRxatIhu2lWBzt8s-dQvGI1yuKsuGfaZH85HCv2i3-dZOP4Ork-OvR6eh780QmjQW01BqjQ4vmjaVSqUiF9GZXCNiPXCE_2NFHrss1zxXWmnNIxunuuRkGpWJEztInkOvwqe-BYww79BLcLHDCDkXTuU554qXOsNBIsoAko4ghfHA5dQ_Y1y02TiJAcziMRdExsKTMYBw-avJArjjP-sPidbLtQS73X5R390UXoqLWDqTyFxabiNeDmTusqhUyoiS81RzHcAWcUq3QVP85toAtjvuuX_67XIaBZyyNqpy9awhBFfyujIpA3ixYLblRWL8SIliHoBcYcOVu1idqUbfWxDxBONy3Pvlvy_rFTxCDzGmApk42obe9G7mXqMXNtVvvGj9Asa7Maw priority: 102 providerName: ProQuest |
| Title | Longitudinal qPCR Study of the Dynamics of L. crispatus, L. iners, A. vaginae, (Sialidase Positive) G. vaginalis, and P. bivia in the Vagina |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/23028904 https://www.proquest.com/docview/1326550835 https://www.proquest.com/docview/1082406877 https://pubmed.ncbi.nlm.nih.gov/PMC3448655 https://doaj.org/article/27ec3797d4d14f079e81faac6f445b4b http://dx.doi.org/10.1371/journal.pone.0045281 |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3fa9swEBZd-7KXse5XvXVBgz20UJtYliX7YYw2NC2jK6EskDcjWfJmCHYbJ2H7H_ZH7062wzJS9mKiSLIS6c5355O-j5CPKk_Bquexn3CIdLhR0lcQNfjDPDZS6Bzcfnw18PVWXE_5l1k82yM9Z2s3gc3O0A75pKaLefDz4ddnUPhPjrVBhn2n4L6ubOAwwvEs9oHLGOFmPr7JK4B2C9EdoHusJ8IDRy7_xrdslYP0RwjUed3sckf_3VX5l5kaPyfPOv-SnrcCcUj2bPWCHHYa3NCTDmb69CX5fVMjV9HKIC8WfZiM7qjDmqV1QcErpKblqm-wfBNQeLrAw2e5as6whEcG4dN5QNdIc6TsGT1pQJRLA1aRtjvB1vaUXvUN5iU0V5Whk4Dqcl0quIUbpq1-Rabjy2-ja7_jZvDzmImlL7UGhxdMm4qlUqEN8UxuLpgeWsT_MSJlNkk1T5VWWvPQsFgXHE2jyllkhtFrsl_BrB8Riph34CVYZiFCToVVacq54oVOoBCJwiNRvwpZ3gGXI3_GPHPZOAkBTDu3GS5j1i2jR_xNr_sWuOM_7S9wgTdtEXbbfVEvvmedFmdM2jySqTTchLwYytQmYaFULgrOY821R45QPPoBGhiOiRgR92OPHPcis7v6w6YaFByzNqqy9apBBFf0uhIpPfKmlbDNj-wF1SNyS_a2_sV2TVX-cCDiEcTlMPbbR-_5jjwF55Dh3hgWHpP95WJl34MDttQD8kTOJFyTUYjX8dWAHFxc3k7uBu6VxsDp3B9ZHzNM |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6VVAIuiJZHDQUWCaRWqtPYXnvjQ4X6JKVJFJUW9Wb2ZbAU7LROgvof-E38NmacdSCoglNv2ezGdjyz89iZ-YaQN0LFoNVV6LYZeDpMC-4K8Brclgo1j6QCsx-PBnr9qHPOPlyEF0vkZ10Lg2mVtUysBLUuFJ6Rb4PXFIWIXR6-G1262DUKo6t1Cw1hWyvonQpizBZ2nJjr7-DClTvHB0Dvt75_dHi233FtlwFXhX40drmUYLqBkBYhF8IzHlaXqsiXLYNINjqKfdOOJYuFFFIyT_uhTBkKeaH8QLcCuO4dsszwAKVBlvcO-4PTWheANIkiW7AXcG_b8kdzVOSmWaGZt70FhVj1DUCc1WFR3mTz_p26-YcuPHpIHlgjlu7OuG6FLJl8ldzt2TD9KlmxEqOkGxbWevMR-dEtsDfSRGMfLno52D-lmMR4TYuUghVKD65z8S1TJY67TQrSDITdeFJu4QhLFOHTbpNOsa2SMFt04yNsnUyDFqaDKvNsajbp-3rBMIPlItd00KQym2YCLlHd5lM1_Zic3wqVnpBGDm99jVDE2AOrxPgGPPI4MiKOGRMslW0YBFHqkKAmSKIsUDr26xgmVfSPg8M0e80JkjGxZHSIO__VaAYU8p_1e0jr-VqE-a6-KK6-JFZqJD43KuAx10x7LG3x2LS9VAgVpYyFkkmHrCGn1Dcok9-7xCHrNffcPP16Pg0CBaNEIjfFpETEWLTy2pw75OmM2eYPCf4qBqaZQ_gCGy78i8WZPPtagZYHjGEN9LN_P9Yrcq9z1usm3eP-yXNyH6xTH5NzfG-dNMZXE_MCLMCxfGm3GSWfb3tn_wJAQ24v |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3dT9NQFL9BSIgvRvCDKuo10QQSuq3tbe_6QAwwJshYFhTCW71f1SazHXTD7H_wL_Ov8pzudjpD9Im33t67tuv57j3ndwh5I1QMVl2FbptBpMO04K6AqMFtqVDzSCpw-_HTwGk_OjpnHy7DyyXys66FwbTKWidWiloXCr-RNyFqikLELg-bqU2LGHS670ZXLnaQwp3Wup2GsG0W9G4FN2aLPE7M9DuEc-XucQdo_9b3u4efDo5c23HAVaEfjV0uJbhxoLBFyIXwjIeVpiryZcsgqo2OYt-0Y8liIYWUzNN-KFOGCl8oP9CtAK57j6xwsPoQCK7sH_YHZ7VdAM0SRbZ4L-Be0_JKY1TkplEhm7e9BeNY9RBAzNVhUd7m__6dxvmHXew-JA-sQ0v3Zhy4RpZMvk5WT-2W_TpZs9qjpFsW4nr7EfnRK7BP0kRjTy56NTg4o5jQOKVFSsEjpZ1pLr5lqsRxr0FBs4HiG0_KHRxhuSIc7TXoDbZYEmaHbn0EMco0WGQ6qLLQbsw2fV8vGGawXOSaDhpUZjeZgEtUt7moph-T8zuh0hOynMNb3yAU8fbAQzG-geg8joyIY8YES2UbBkGUOiSoCZIoC5qOvTuGSbUTyCF4mr3mBMmYWDI6xJ3_ajQDDfnP-n2k9XwtQn5XJ4rrL4nVIInPjQp4zDXTHktbPDZtLxVCRSljoWTSIRvIKfUNyuS3xDhks-ae26dfz6dBueCOkchNMSkRPRY9vjbnDnk6Y7b5Q0LsipvUzCF8gQ0X_sXiTJ59rQDMA8awHvrZvx_rFVkFCU96x_2T5-Q-OKo-5un43iZZHl9PzAtwBsfypZUySj7ftWD_ApWicnM |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Longitudinal+qPCR+study+of+the+dynamics+of+L.+crispatus%2C+L.+iners%2C+A.+vaginae%2C+%28sialidase+positive%29+G.+vaginalis%2C+and+P.+bivia+in+the+vagina&rft.jtitle=PloS+one&rft.au=Santiago%2C+Guido+Lopes+dos+Santos&rft.au=Tency%2C+Inge&rft.au=Verstraelen%2C+Hans&rft.au=Verhelst%2C+Rita&rft.date=2012-09-21&rft.eissn=1932-6203&rft.volume=7&rft.issue=9&rft.spage=e45281&rft_id=info:doi/10.1371%2Fjournal.pone.0045281&rft_id=info%3Apmid%2F23028904&rft.externalDocID=23028904 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |