Single-Laboratory Validation Study of Rapid Analysis Method for Multi-Class Veterinary Drugs in Milk, Fish and Shellfish by LC-MS/MS

A method of rapid analysis of multi-class residual veterinary drugs in milk, fish and shellfish was validated in accordance with Japanese guidelines for the validation of analytical methods for residual agricultural chemicals in food. Using LC-MS/MS, 43 multi-class veterinary drugs, including sulfon...

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Published inFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 54; no. 4; pp. 335 - 344
Main Authors TATEISHI, Yukinari, HASHIMOTO, Tsuneo, MATSUSHIMA, Yoko, TAKANO, Ichiro, NAGANO, Chieko, KANDA, Maki, HAYASHI, Hiroshi, KANAI, Setsuko, SASAMOTO, Takeo, NAKAJIMA, Takayuki
Format Journal Article
LanguageEnglish
Published Japan Japanese Society for Food Hygiene and Safety 25.08.2013
Subjects
Animals
Chromatography, High Pressure Liquid - methods
Drug Residues - analysis
fish and shellfish
Fish Products - analysis
Fishes - metabolism
Food Analysis - methods
Food Analysis - standards
Food Contamination - analysis
Guidelines as Topic - standards
Japan
LC-MS/MS
milk
Milk - chemistry
multi-class analysis
Salmonidae
Shellfish - analysis
single-laboratory validation study
Tandem Mass Spectrometry - methods
veterinary drug
Veterinary Drugs - analysis
Japan
Online AccessGet full text
ISSN0015-6426
1882-1006
1882-1006
DOI10.3358/shokueishi.54.335

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Abstract A method of rapid analysis of multi-class residual veterinary drugs in milk, fish and shellfish was validated in accordance with Japanese guidelines for the validation of analytical methods for residual agricultural chemicals in food. Using LC-MS/MS, 43 multi-class veterinary drugs, including sulfonamides, quinolones, coccidiostats and antiparasites, could be analyzed in one injection. Analytes were extracted from samples with two kinds of solvent, acetonitrile containing 1 vol% formic acid and anhydrous acetonitrile, and salted out with 4.0 g of magnesium sulfate, 1.5 g of trisodium citrate and 2.0 g of sodium chloride. This method was assessed by performing recovery tests in retail milk and 4 kinds of fresh cultured fish and shellfish (salmon, tiger shrimp, red sea bream and bastard halibut) spiked with the 43 target analytes at the levels of 10 and 100 μg/kg. Using this method, 40 out of 43 drugs satisfied the guideline criteria in milk, 37 drugs in salmon, 42 drugs in tiger shrimp, 41 drugs in red sea bream and 39 drugs in bastard halibut.
AbstractList A method of rapid analysis of multi-class residual veterinary drugs in milk, fish and shellfish was validated in accordance with Japanese guidelines for the validation of analytical methods for residual agricultural chemicals in food. Using LC-MS/MS, 43 multi-class veterinary drugs, including sulfonamides, quinolones, coccidiostats and antiparasites, could be analyzed in one injection. Analytes were extracted from samples with two kinds of solvent, acetonitrile containing 1 vol% formic acid and anhydrous acetonitrile, and salted out with 4.0 g of magnesium sulfate, 1.5 g of trisodium citrate and 2.0 g of sodium chloride. This method was assessed by performing recovery tests in retail milk and 4 kinds of fresh cultured fish and shellfish (salmon, tiger shrimp, red sea bream and bastard halibut) spiked with the 43 target analytes at the levels of 10 and 100 μg/kg. Using this method, 40 out of 43 drugs satisfied the guideline criteria in milk, 37 drugs in salmon, 42 drugs in tiger shrimp, 41 drugs in red sea bream and 39 drugs in bastard halibut.
A method of rapid analysis of multi-class residual veterinary drugs in milk, fish and shellfish was validated in accordance with Japanese guidelines for the validation of analytical methods for residual agricultural chemicals in food. Using LC-MS/MS, 43 multi-class veterinary drugs, including sulfonamides, quinolones, coccidiostats and antiparasites, could be analyzed in one injection. Analytes were extracted from samples with two kinds of solvent, acetonitrile containing 1 vol% formic acid and anhydrous acetonitrile, and salted out with 4.0 g of magnesium sulfate, 1.5 g of trisodium citrate and 2.0 g of sodium chloride. This method was assessed by performing recovery tests in retail milk and 4 kinds of fresh cultured fish and shellfish (salmon, tiger shrimp, red sea bream and bastard halibut) spiked with the 43 target analytes at the levels of 10 and 100 mu g/kg. Using this method, 40 out of 43 drugs satisfied the guideline criteria in milk, 37 drugs in salmon, 42 drugs in tiger shrimp, 41 drugs in red sea bream and 39 drugs in bastard halibut.
Author NAGANO, Chieko
MATSUSHIMA, Yoko
SASAMOTO, Takeo
KANAI, Setsuko
HAYASHI, Hiroshi
TATEISHI, Yukinari
TAKANO, Ichiro
HASHIMOTO, Tsuneo
KANDA, Maki
NAKAJIMA, Takayuki
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Cites_doi 10.1016/j.foodchem.2011.12.042
10.3358/shokueishi.52.34
10.1021/jf301723g
10.3358/shokueishi.49.381
10.1016/j.aca.2009.12.016
10.1016/j.chroma.2011.01.021
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11
1
12
2
3
4
5
6
7
8
9
10
References_xml – reference: 5) Jimenez, V., Rubies, A., Centrich, F., Companyo, R., Guiteras, J. Development and validation of a amulticlass method for the analysis of antibiotic residues in eggs by liquid chromatography-tandem mass spectrometry. J. Chromatogr. A, 1218, 1443–1451 (2011).
– reference: 9) Kanda, M., Sasamoto, T., Takeba, K., Hayashi, H., Kusano, T., Matsushima, Y., Nakajima, T., Kanai, S., Takano, I. Rapid determination of fumagillin residue in honey by liquid chromatography-tandem mass spectrometry using the QuEChERS method. J. AOAC Int., 94, 878–885 (2011).
– reference: 6) Aguilera-Luiz, Martinez Vidal, J. L., M. M., Romero-Gonzalez, R., Frenich, A. G. Multiclass method for fast determination of veterinary drug residues in baby food by ultra-high-performance liquid chromatography-tandem mass spectrometry. Food Chem., 132, 2171–2180 (2012).
– reference: 7) Chen, Y., Al-Taher, F., Juskelis, R., Wong, J. W., Zhang, K., Hayward, D. G., Zweigenbaum, J., Stevens, J. Multiresidue pesticide analysis of dried botanical dietary supplements using an automated dispersive SPE cleanup for QuEChERS and high-performance liquid chromatography-tandem mass spectrometry. J. Agric. Food Chem., 60, 9991–9999 (2012).
– reference: 4) Frenich, A.G., Aguilera-Luiz, M.M., Martinez Vidal, J.L., Romero-Gonzalez, R. Comparison of several extraction techniques for multiclass analysis of veterinary drugs in eggs using ultra-high pressure liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta, 661, 150–160 (2010).
– reference: 3) Kajita, H., Hatakeyama, E. Simultaneous determination of residual veterinary drugs in livestock products and fish by liquid chromatography with tandem mass spectrometry. J. Food Hyg. Soc. Japan, 49, 381–389 (2008).
– reference: 12) Nakajima, T., Sasamoto, T., Hayashi, H., Kanda, M., Takeba, K., Kanai, S., Kusano, T., Matsushima, Y., Takano, I. Screening assay of residual antibiotics in livestock samples by LC-MS/MS. Food Hyg. Saf. Sci., 53, 91–97 (2012).
– reference: 10) Saito, S., Sakai, T., Nemoto, S., Matsuda, R. Determination of pindone in animal products, fishery products and honey by LC-MS/MS. Food Hyg. Saf. Sci., 52, 294–298 (2011).
– reference: 11) Nakajima, T., Nagano, C., Sasamoto, T., Hayashi, H., Kanda, M., Kanai, S., Takeba, K., Matsushima, Y., Takano, I. Determination and validation of rapid analysis method for multi-class veterinary drugs in livestock products by LC-MS/MS. Food Hyg. Saf. Sci., 53, 243–253 (2012).
– reference: 1) Wegener, H. C., Aarestrup, F. M., Gerner-Smidt, P., Bager, F. Transfer of antibiotic resistant bacteria from animals to man. Acta Vet. Scand. Suppl., 92, 51–56 (1999).
– reference: 2) Noguchi, S., Terada, H., Tamura, Y. Simultaneous determination of veterinary drugs in livestock foods and seafoods using liquid chromatography/tandem mass spectrometry. J. Food Hyg. Soc. Japan, 49, 177–188 (2008).
– reference: 8) Ishii, R., Takahashi, K., Matsumoto, R. Analysis of imidocarb in livestock and seafood products using LC-MS/MS. Food Hyg. Saf. Sci., 52, 34–39 (2011).
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SubjectTerms Animals
Chromatography, High Pressure Liquid - methods
Drug Residues - analysis
fish and shellfish
Fish Products - analysis
Fishes - metabolism
Food Analysis - methods
Food Analysis - standards
Food Contamination - analysis
Guidelines as Topic - standards
Japan
LC-MS/MS
milk
Milk - chemistry
multi-class analysis
Salmonidae
Shellfish - analysis
single-laboratory validation study
Tandem Mass Spectrometry - methods
veterinary drug
Veterinary Drugs - analysis
Title Single-Laboratory Validation Study of Rapid Analysis Method for Multi-Class Veterinary Drugs in Milk, Fish and Shellfish by LC-MS/MS
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