Directed Evolution of Protein Enzymes Using Nonhomologous Random Recombination - Bittker, Joshua A.; Le, Brian V.; Liu, Jane M.; Liu, David R.; Joyce, Gerald F. (Proceedings of the National Academy of Sciences - PNAS, 2004)
Keywords: Biological Sciences; Chorismate Mutase - genetics; Crossovers; Dimers; Directed Molecular Evolution; DNA; Enzymes; Escherichia coli - chemistry; Escherichia coli - genetics; Escherichia coli - metabolism; Evolution; Libraries; Ligation; Mutation; Nucleic acids; Plasmids; Polymerase chain reaction; Protein Structure, Tertiary; Proteins; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism