Optimal RNA isolation method and primer design to detect gene knockdown by qPCR when validating Drosophila transgenic RNAi lines - Mainland, Roslyn L.; Lyons, Taylor A.; Ruth, Mike M.; Kramer, Jamie M. (BMC research notes, 2017)
Keywords: Analysis; Biomedical and Life Sciences; Biomedicine; Drosophila; Drosophila transgenic RNAi lines; Genes; Genetic aspects; Genetic engineering; Genetic research; Life Sciences; Medicine/Public Health; Messenger RNA; Methods; Proteins; Quantitative real-time PCR; Research Note; RNA interference; RNAi knockdown efficiency; UAS-Gal4 system; Quantitative real-time PCR; transgenic RNAi lines; RNAi knockdown efficiency; UAS-Gal4 system; Drosophila transgenic RNAi lines