Association of GSTM1 and GSTT1 polymorphisms with DNA damage in coal-tar workers

• Published in: The Science of the total environment Vol. 409; no. 20; pp. 4465 - 4469
• Main Authors: Giri, Shiv Kumar, Anita Yadav, Kumar, Anil, Dev, Kapil, Gupta, Ranjan, Aggarwal, Neeraj, Seth, Neena, Gautam, Sanjeev Kumar
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.09.2011; Elsevier
• Subjects: Adult; adverse effects; Alkaline comet assay; analysis; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Chemical agents; Chemical mutagenesis; Coal Tar; Comet Assay; Damage; Damage detection; Deoxyribonucleic acid; DNA Damage; Exposure; Female; Genes; genetics; genotype; glutathione transferase; Glutathione Transferase - genetics; GSTM1; GSTT1; Humans; labor; Lymphocytes; Male; Medical sciences; Middle Aged; Occupational Exposure; Occupational Exposure - adverse effects; Occupational Exposure - analysis; Polyallylamine hydrochloride; Polycyclic aromatic hydrocarbon; polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - toxicity; Polymorphism; Polymorphism, Genetic; Tail moment; toxicity; Toxicology; Tumors; Workplace; Workplace - standards; Young Adult; Asia; India; LMA; NMA; GSTM1; Polycyclic aromatic hydrocarbon; DNA damage; SCGE; GST; GSTT1; B [a] P; SD; CPCB; Tail moment; Alkaline comet assay; TM; PAHs; NAAQS; TSPM; Toxicity; Occupational exposure; Mutagen; Comet assay; Persistent organic pollutant; Health and environment; Toxicogenetics; Predisposition; Genetics; Worker; Public health; Organic compounds; Genotoxity test; Human; Hydrocarbon; Polycyclic aromatic compound; Carcinogen; DNA; Coal tar; Occupational medicine; Polymorphism
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2011.07.009

DNA damage was evaluated by alkaline comet assay in peripheral blood lymphocytes of 115 coal-tar workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs) and 105 control subjects. The effect of polymorphisms of glutathione S-transferase ( GST) genotypes on the DNA damage was assessed. The mean tail moment (TM) value in the coal-tar workers was significantly higher as compared to the control subjects (12.06 ± 0.55 versus 0.44 ± 0.31; P < 0.05). No significant association ( P > 0.05) between the GSTT1 and GSTM1 genotypes and the TM values was found, however highest mean rank TM value was reported in GSTM1 null and GSTT1 null genotypes in both control and exposed subjects. Our results suggest that there is increased DNA damage in coal-tar workers due to PAHs exposure. Polymorphisms in GSTM1 and GSTT1 genes do not show significant effect ( P > 0.05) on DNA damage. ► Coal-tar workers show high DNA damage in Peripheral blood lymphocytes. ► Exposed subjects with long exposure duration show more DNA damage. ► GSTM1 and GSTT1 positive genotypes are more prevalent among studied subjects. ► Polymorphisms in GSTM1 and GSTT1 genes do not show significant effect on DNA damage.