The detection of Mycoplasma ( formerly Eperythrozoon) wenyonii by 16S rDNA PCR and denaturing gradient gel electrophoresis
Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously,...
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| Published in | Veterinary microbiology Vol. 117; no. 2; pp. 292 - 296 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
31.10.2006
Elsevier Science |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0378-1135 1873-2542 |
| DOI | 10.1016/j.vetmic.2006.06.011 |
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| Abstract | Although the role of
Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of
M. wenyonii has been based on blood smears but is not specific for
M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of
Mycoplasma species. DGGE enables the rapid and specific identification of
Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect
M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for
M. wenyonii and can also be used as a screening test for other blood borne pathogens. |
|---|---|
| AbstractList | Although the role of
Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of
M. wenyonii has been based on blood smears but is not specific for
M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of
Mycoplasma species. DGGE enables the rapid and specific identification of
Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect
M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for
M. wenyonii and can also be used as a screening test for other blood borne pathogens. Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of M. wenyonii has been based on blood smears but is not specific for M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of Mycoplasma species. DGGE enables the rapid and specific identification of Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for M. wenyonii and can also be used as a screening test for other blood borne pathogens. Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of M. wenyonii has been based on blood smears but is not specific for M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of Mycoplasma species. DGGE enables the rapid and specific identification of Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for M. wenyonii and can also be used as a screening test for other blood borne pathogens.Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of M. wenyonii has been based on blood smears but is not specific for M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of Mycoplasma species. DGGE enables the rapid and specific identification of Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for M. wenyonii and can also be used as a screening test for other blood borne pathogens. |
| Author | Nicholas, Robin Ayling, Roger Barlow, Alex McAuliffe, Laura Lawes, Joanna Bell, Suzanna |
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| Cites_doi | 10.1099/jmm.0.46058-0 10.1128/JCM.42.8.3775-3780.2004 10.1128/AEM.64.2.795-799.1998 10.1128/JCM.41.10.4844-4847.2003 10.1099/00207713-51-3-891 10.1073/pnas.80.6.1579 10.2460/javma.1994.204.02.261 10.1111/j.1574-6968.1997.tb12742.x 10.2460/javma.1990.196.08.1244 10.1016/0304-4017(93)90013-D |
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| Keywords | Eperythrozoon Mycoplasma wenyonii DGGE Microbiology Ribosomal DNA Anaplasmataceae Denaturing gradient gel electrophoresis Mycoplasma Mycoplasmataceae Mollicutes Rickettsiales Polymerase chain reaction Mycoplasmatales Bacteria Detection Veterinary Eperythrozoon wenyonii |
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Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal... Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal... |
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| SubjectTerms | animal pathogenic bacteria Animals Bacteriology Base Sequence Biological and medical sciences cattle diseases dairy cattle denaturing gradient gel electrophoresis DGGE diagnostic techniques disease detection disease diagnosis DNA, Bacterial - chemistry DNA, Ribosomal - chemistry Electrophoresis, Agar Gel - methods Electrophoresis, Agar Gel - veterinary Eperythrozoon Fundamental and applied biological sciences. Psychology microbial genetics Microbiology Miscellaneous Molecular Sequence Data Mycoplasma Mycoplasma - chemistry Mycoplasma - classification Mycoplasma - genetics Mycoplasma - isolation & purification Mycoplasma Infections - diagnosis Mycoplasma Infections - veterinary Mycoplasma wenyonii mycoplasmosis pathogen identification Phylogeny polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary ribosomal DNA RNA, Ribosomal, 16S - chemistry screening Sensitivity and Specificity Species Specificity strain differences Time Factors |
| Title | The detection of Mycoplasma ( formerly Eperythrozoon) wenyonii by 16S rDNA PCR and denaturing gradient gel electrophoresis |
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