Excitability parameters and sensitivity to anemone toxin ATX-II in rat small diameter primary sensory neurones discriminated by Griffonia simplicifolia isolectin IB4

• Published in: The Journal of physiology Vol. 588; no. 1; pp. 125 - 137
• Main Authors: Snape, Alistair, Pittaway, James F., Baker, Mark D.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 01.01.2010; Blackwell Publishing Ltd; Wiley Subscription Services, Inc; Blackwell Science Inc
• Subjects: Action Potentials - drug effects; Animals; Cell Size; Cells, Cultured; Cnidarian Venoms - toxicity; Dose-Response Relationship, Drug; Ganglia, Spinal - drug effects; Ganglia, Spinal - physiology; Neuroscience; Neurotoxins - toxicity; Plant Lectins - toxicity; Rats; Rats, Wistar; Sensory Receptor Cells - cytology; Sensory Receptor Cells - drug effects; Sensory Receptor Cells - physiology
• ISSN: 0022-3751; 1469-7793; 1469-7793
• DOI: 10.1113/jphysiol.2009.181107

Sensory neurone subtypes (≤ 25 μm apparent diameter) express a variety of Na + channels, where expression is linked to action potential duration, and associated with differential IB4-lectin binding. We hypothesized that sensitivity to ATX-II might also discriminate neurones and report that 1 μ m has negligible or small effects on action potentials in IB4 +ve, but dramatically increased action potential duration in IB4 −ve, neurones. The toxin did not act on tetrodotoxin-resistant (TTX-r) Na V 1.8 currents; discrimination was based on tetrodotoxin-sensitive (TTX-s) Na + channel expression. We also explored the effects of varying the holding potential on current threshold, and the effect of repetitive activation on action currents in IB4 +ve and −ve neurones. IB4 +ve neurones became more excitable with depolarization over the range −100 to −20 mV, but IB4 −ve neurones exhibited peak excitability near −55 mV, and were inexcitable at −20 mV. Eliciting action potentials at 2 Hz, we found that peak inward action current in IB4 +ve neurones was reduced, whereas changes in the current amplitude were negligible in most IB4 −ve neurones. Our findings are consistent with relatively toxin-insensitive channels including Na V 1.7 being expressed in IB4 +ve neurones, whereas toxin sensitivity indicates that IB4 −ve neurones may express Na V 1.1 or Na V 1.2, or both. The retention of excitability at low membrane potentials, and the responses to repetitive stimulation are explained by the known preferential expression of Na V 1.8 in IB4 +ve neurones, and the reduction in action current in IB4 +ve neurones with repetitive stimulation supports a novel hypothesis explaining the slowing of conduction velocity in C-fibres by the build-up of Na + channel inactivation.