Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators

Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the re...

Full description

Saved in:
Bibliographic Details
Published inNature communications Vol. 11; no. 1; pp. 5107 - 18
Main Authors Li, Li-Li, Klein, Florence M., Li Greci, Lorenzo, Popinigis, Arkadiusz, Freudenberg, Florian, Courtney, Michael J.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 09.10.2020
Nature Portfolio
Subjects
13/95
14
14/63
631/1647/2253
631/61/338/469
631/92/552
96/10
96/33
96/35
96/95
Animals
Energy Transfer
Female
Fluorescence Resonance Energy Transfer - methods
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HEK293 Cells
Hippocampus - cytology
Humanities and Social Sciences
Humans
Light
Male
MAP Kinase Signaling System
Molecular Imaging - methods
multidisciplinary
Neurons
Optogenetics - methods
Phototropins - analysis
Phototropins - genetics
Phototropins - metabolism
Rats
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Science
Science (multidisciplinary)
Online AccessGet full text
ISSN2041-1723
2041-1723
DOI10.1038/s41467-020-18816-8

Cover

Abstract Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ–measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision. Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use resonance energy transfer to overcome these concerns.
AbstractList Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ–measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision. Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use resonance energy transfer to overcome these concerns.
Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ–measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision. Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use resonance energy transfer to overcome these concerns.
Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ-measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision.
Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ-measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision.Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high spatiotemporal resolution, reversibility and repeatability. Cellular optogenetics applications remain limited with diffusible targets as the response of the actuator is difficult to independently validate. Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. We describe a simple approach overcoming these obstacles. Resonance energy transfer can be used to constitutively or dynamically modulate actuation sensitivity. This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. Applying this approach to different LOV2-based switches reveals that flanking sequences can lead to relaxation times up to 11-fold faster than anticipated. In situ-measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision.
Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use resonance energy transfer to overcome these concerns.
ArticleNumber 5107
Author Li Greci, Lorenzo
Klein, Florence M.
Li, Li-Li
Popinigis, Arkadiusz
Freudenberg, Florian
Courtney, Michael J.
Author_xml – sequence: 1
  givenname: Li-Li
  orcidid: 0000-0002-8888-153X
  surname: Li
  fullname: Li, Li-Li
  organization: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University, Turku Screening Unit, Metabolic Research Laboratories, Wellcome-MRC Institute of Metabolic Science, University of Cambridge
– sequence: 2
  givenname: Florence M.
  surname: Klein
  fullname: Klein, Florence M.
  organization: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
– sequence: 3
  givenname: Lorenzo
  orcidid: 0000-0002-1909-7645
  surname: Li Greci
  fullname: Li Greci, Lorenzo
  organization: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
– sequence: 4
  givenname: Arkadiusz
  surname: Popinigis
  fullname: Popinigis, Arkadiusz
  organization: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University, BLIRT S.A
– sequence: 5
  givenname: Florian
  orcidid: 0000-0003-1438-3850
  surname: Freudenberg
  fullname: Freudenberg, Florian
  organization: Department of Psychiatry, Psychosomatic Medicine and Psychotherapy, University Hospital, Goethe University
– sequence: 6
  givenname: Michael J.
  orcidid: 0000-0001-8693-3933
  surname: Courtney
  fullname: Courtney, Michael J.
  email: michael.courtney@bioscience.fi
  organization: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University, Turku Screening Unit
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33037199$$D View this record in MEDLINE/PubMed
BookMark eNqNkV1vFCEUhiemxtbaP-CF4dKbUb4GmBsT0_jRZJMmRr0lDJyZspmFFRib_fey3bVpvWjkBnI47_MeeF82JyEGaJrXBL8jmKn3mRMuZIspbolSRLTqWXNGMSctkZSdPDifNhc5r3FdrCeK8xfNKWOYSdL3Z830DXIMJlhAECBNO1SSCXmEhDKE7IvPkJEJDm1i8CWmjHxAtb6gfOuLvfFhQnFEq-uftB1MBofitsSpwoq3yNiymL3qVfN8NHOGi-N-3vz4_On75dd2df3l6vLjqrUdoaV11jllGHA-yhEzTiVVjAk6KGrdMCgYsLSdpR0jI7GDYkriHsNgjWWYGMfOm6sD10Wz1tvkNybtdDRe3xVimrRJdbIZ9OgsswqPggnBmZGVBoIpMWBhqkFXWezAWsLW7G7NPN8DCdb7FPQhBV1T0HcpaFVVHw6q7TJswFkI9UfnR6M8vgn-Rk_xt5Ydl5LyCnh7BKT4a4Fc9MZnC_NsAsQla8p533cKC1lb3zz0ujf5m29toIcGm2LOCcb_e4H6R2R9McXH_bx-flp6_LJcfcIESa_jkkJN_CnVH8zb3EE
CitedBy_id crossref_primary_10_1002_adbi_202400416
crossref_primary_10_3390_biom11030343
crossref_primary_10_1021_acssynbio_1c00350
crossref_primary_10_7554_eLife_87303
crossref_primary_10_7554_eLife_87303_3
Cites_doi 10.1016/j.cell.2014.04.039
10.1038/ncomms14396
10.1371/journal.pone.0082693
10.1016/S0074-7742(09)85014-9
10.1074/jbc.M103114200
10.1016/j.ynpai.2019.100034
10.1038/s41598-017-07002-4
10.1016/j.chembiol.2012.02.006
10.1016/j.cell.2016.11.054
10.1126/science.1102506
10.1021/bi060093i
10.1038/nbt.1493
10.1021/bi00851a021
10.1117/12.548054
10.1097/j.pain.0000000000001152
10.1126/science.1086810
10.1021/acs.jpclett.6b01484
10.1016/j.cels.2020.02.005
10.1038/s41467-020-17836-8
10.1021/bi047881x
10.1038/ncomms10624
10.1038/s41467-018-04754-z
10.1371/journal.pone.0087074
10.1016/j.jmb.2012.02.037
10.1038/s41592-019-0352-8
10.1111/j.1751-1097.2011.00903.x
10.1523/JNEUROSCI.4578-12.2013
10.1016/j.cub.2018.11.053
10.1016/j.euroneuro.2016.01.008
10.3389/fmolb.2015.00018
10.1529/biophysj.104.058230
10.1038/nmeth.3926
10.1038/nmeth819
10.1038/s41592-019-0541-5
10.1021/acs.chemrev.8b00163
10.1091/mbc.E15-12-0832
10.1083/jcb.201612094
10.1083/jcb.200407024
10.1038/nchembio.210
10.1038/nmeth.1904
10.1038/nature09198
10.1038/ncomms15017
10.1038/nature08241
10.1007/978-1-4939-7154-1_1
10.1016/j.neuron.2017.02.036
10.1038/nn1869
10.1016/j.bpj.2013.07.010
10.5281/zenodo.3882572
10.1523/ENEURO.0386-19.2019
10.21203/rs.3.pex-1131/v1
10.1523/ENEURO.0303-19.2019
10.1021/acschembio.0c00543
ContentType Journal Article
Copyright The Author(s) 2020
Copyright_xml – notice: The Author(s) 2020
DBID C6C
AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7X8
5PM
ADTOC
UNPAY
DOA
DOI 10.1038/s41467-020-18816-8
DatabaseName Springer Nature OA Free Journals
CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
PubMed Central (Full Participant titles)
Unpaywall for CDI: Periodical Content
Unpaywall
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList

MEDLINE
MEDLINE - Academic

CrossRef
Database_xml – sequence: 1
  dbid: C6C
  name: Springer Nature OA Free Journals
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: DOA
  name: DOAJ Directory of Open Access Journals [Accès libre]
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 3
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 4
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 5
  dbid: UNPAY
  name: Unpaywall
  url: https://proxy.k.utb.cz/login?url=https://unpaywall.org/
  sourceTypes: Open Access Repository
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2041-1723
EndPage 18
ExternalDocumentID oai_doaj_org_article_fdc3c80f636643a7b83e6386b06a2535
10.1038/s41467-020-18816-8
PMC7547724
33037199
10_1038_s41467_020_18816_8
Genre Video-Audio Media
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: Academy of Finland (Suomen Akatemia)
  grantid: 309736, 324581
  funderid: https://doi.org/10.13039/501100002341
– fundername: Magnus Ehrnroothin Säätiö (Magnus Ehrnrooth Foundation)
  funderid: https://doi.org/10.13039/501100004155
– fundername: U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
– fundername: 7th Framework Programme Initial Training Networks FP7-PEOPLE-2013-ITN Project Number 608346 Project ‘Brain Imaging Return To Health’ r’Birth
– fundername: EC | Erasmus+
  funderid: https://doi.org/10.13039/501100010790
– fundername: U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
  grantid: R01CA200417; R01CA200417
  funderid: https://doi.org/10.13039/100000054
– fundername: NCI NIH HHS
  grantid: R01 CA200417
– fundername: ;
– fundername: ;
  grantid: 309736, 324581
– fundername: ;
  grantid: R01CA200417; R01CA200417
GroupedDBID ---
0R~
39C
3V.
53G
5VS
70F
7X7
88E
8AO
8FE
8FG
8FH
8FI
8FJ
AAHBH
AAJSJ
ABUWG
ACGFO
ACGFS
ACIWK
ACMJI
ACPRK
ACSMW
ADBBV
ADFRT
ADMLS
ADRAZ
AENEX
AEUYN
AFKRA
AFRAH
AHMBA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMTXH
AOIJS
ARAPS
ASPBG
AVWKF
AZFZN
BBNVY
BCNDV
BENPR
BGLVJ
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
EBLON
EBS
EE.
EMOBN
F5P
FEDTE
FYUFA
GROUPED_DOAJ
HCIFZ
HMCUK
HVGLF
HYE
HZ~
KQ8
LK8
M1P
M48
M7P
M~E
NAO
O9-
OK1
P2P
P62
PIMPY
PQQKQ
PROAC
PSQYO
RNS
RNT
RNTTT
RPM
SNYQT
SV3
TSG
UKHRP
AASML
AAYXX
CITATION
PHGZM
PHGZT
PJZUB
PPXIY
PQGLB
PUEGO
CGR
CUY
CVF
ECM
EIF
NPM
7X8
5PM
4.4
ADTOC
BAPOH
CAG
COF
EJD
LGEZI
LOTEE
NADUK
NXXTH
UNPAY
ID FETCH-LOGICAL-c512t-dcdd8a3e44f7f03427283362b82cdbb8eb07c5c2531f1cb8387090ebcac301ad3
IEDL.DBID M48
ISSN 2041-1723
IngestDate Fri Oct 03 12:53:12 EDT 2025
Sun Oct 26 04:03:08 EDT 2025
Tue Sep 30 15:37:31 EDT 2025
Fri Sep 05 10:48:12 EDT 2025
Mon Jul 21 05:24:34 EDT 2025
Wed Oct 01 04:46:03 EDT 2025
Thu Apr 24 22:59:32 EDT 2025
Fri Feb 21 02:40:04 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
cc-by
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c512t-dcdd8a3e44f7f03427283362b82cdbb8eb07c5c2531f1cb8387090ebcac301ad3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Undefined-3
ORCID 0000-0001-8693-3933
0000-0002-1909-7645
0000-0002-8888-153X
0000-0003-1438-3850
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.1038/s41467-020-18816-8
PMID 33037199
PQID 2449958067
PQPubID 23479
PageCount 18
ParticipantIDs doaj_primary_oai_doaj_org_article_fdc3c80f636643a7b83e6386b06a2535
unpaywall_primary_10_1038_s41467_020_18816_8
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7547724
proquest_miscellaneous_2449958067
pubmed_primary_33037199
crossref_primary_10_1038_s41467_020_18816_8
crossref_citationtrail_10_1038_s41467_020_18816_8
springer_journals_10_1038_s41467_020_18816_8
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2020-10-09
PublicationDateYYYYMMDD 2020-10-09
PublicationDate_xml – month: 10
  year: 2020
  text: 2020-10-09
  day: 09
PublicationDecade 2020
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Nature communications
PublicationTitleAbbrev Nat Commun
PublicationTitleAlternate Nat Commun
PublicationYear 2020
Publisher Nature Publishing Group UK
Nature Portfolio
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Portfolio
References Zayner, Sosnick (CR17) 2014; 9
Strickland (CR24) 2012; 9
Izquierdo, Quinkler, De Renzis (CR32) 2018; 9
Sinnecker, Voigt, Hellwig, Schaefer (CR50) 2005; 44
Zayner, Antoniou, French, Hause, Sosnick (CR3) 2013; 105
Semenova (CR46) 2007; 10
Losi, Gardner, Möglich (CR1) 2018; 118
Kawano, Aono, Suzuki, Sato (CR37) 2013; 8
Jacquemet (CR42) 2019; 29
Kiguchi, Maeda, Kobayashi, Saika, Kishioka (CR28) 2009; 85
Ferris (CR26) 2019; 6
Konold (CR12) 2016; 7
Zoltowski, Vaccaro, Crane (CR23) 2009; 5
Valon, Marín-Llauradó, Wyatt, Charras, Trepat (CR33) 2017; 8
CR2
Grashoff (CR44) 2010; 466
Shaner, Steinbach, Tsien (CR49) 2005; 2
Regot, Hughey, Bajar, Carrasco, Covert (CR22) 2014; 157
Wu (CR29) 2009; 461
Lee (CR27) 2018; 159
CR47
Ando, Mizuno, Miyawaki (CR11) 2004; 306
Goglia (CR5) 2020; 10
Gil (CR19) 2020; 11
Niopek, Wehler, Roensch, Eils, Di Ventura (CR9) 2016; 7
Shin (CR31) 2017; 168
Lambert (CR48) 2019; 16
Lungu (CR8) 2012; 19
Murakoshi (CR7) 2017; 94
CR14
O’Neill, Kalyanaraman, Gautam (CR30) 2016; 27
Goglia, Wilson, DiGiorno, Toettcher (CR34) 2017; 1636
CR52
Pudasaini, El-Arab, Zoltowski (CR35) 2015; 2
van Stokkum (CR39) 2011; 87
Miller, Goebel (CR10) 1968; 7
Harper, Neil, Gardner (CR21) 2003; 301
Candemir (CR45) 2016; 26
Cao (CR51) 2005; 168
Shimozono (CR18) 2006; 45
Wang (CR25) 2016; 13
Melero-Fernandez de Mera (CR6) 2017; 8
Zayner, Antoniou, Sosnick (CR15) 2012; 419
Li (CR41) 2013; 33
Gauden (CR38) 2004; 5463
CR20
Swartz (CR13) 2001; 276
Guo, Kottke, Hegemann, Dick (CR16) 2005; 89
Andresen (CR43) 2008; 26
Bugaj, O’Donoghue, Lim (CR4) 2017; 216
Kinjo (CR36) 2016; 16
Murakoshi, Shibata (CR40) 2017; 7
TJ Lambert (18816_CR48) 2019; 16
JP Zayner (18816_CR15) 2012; 419
S Regot (18816_CR22) 2014; 157
M Andresen (18816_CR43) 2008; 26
AG Goglia (18816_CR5) 2020; 10
A Losi (18816_CR1) 2018; 118
N Kiguchi (18816_CR28) 2009; 85
R Ando (18816_CR11) 2004; 306
A Pudasaini (18816_CR35) 2015; 2
MM Semenova (18816_CR46) 2007; 10
D Strickland (18816_CR24) 2012; 9
Y Shin (18816_CR31) 2017; 168
18816_CR47
JP Zayner (18816_CR17) 2014; 9
CF Ferris (18816_CR26) 2019; 6
WH Lee (18816_CR27) 2018; 159
PE Konold (18816_CR12) 2016; 7
E Candemir (18816_CR45) 2016; 26
PR O’Neill (18816_CR30) 2016; 27
AA Gil (18816_CR19) 2020; 11
YI Wu (18816_CR29) 2009; 461
H Wang (18816_CR25) 2016; 13
RM Melero-Fernandez de Mera (18816_CR6) 2017; 8
18816_CR2
18816_CR14
AG Goglia (18816_CR34) 2017; 1636
JP Zayner (18816_CR3) 2013; 105
LJ Bugaj (18816_CR4) 2017; 216
S Shimozono (18816_CR18) 2006; 45
H Murakoshi (18816_CR40) 2017; 7
18816_CR52
BD Zoltowski (18816_CR23) 2009; 5
M Gauden (18816_CR38) 2004; 5463
LL Li (18816_CR41) 2013; 33
C Grashoff (18816_CR44) 2010; 466
OI Lungu (18816_CR8) 2012; 19
F Kawano (18816_CR37) 2013; 8
NC Shaner (18816_CR49) 2005; 2
G Jacquemet (18816_CR42) 2019; 29
H Murakoshi (18816_CR7) 2017; 94
TE Swartz (18816_CR13) 2001; 276
T Kinjo (18816_CR36) 2016; 16
D Sinnecker (18816_CR50) 2005; 44
D Niopek (18816_CR9) 2016; 7
J Cao (18816_CR51) 2005; 168
WG Miller (18816_CR10) 1968; 7
18816_CR20
L Valon (18816_CR33) 2017; 8
SM Harper (18816_CR21) 2003; 301
H Guo (18816_CR16) 2005; 89
E Izquierdo (18816_CR32) 2018; 9
IH van Stokkum (18816_CR39) 2011; 87
References_xml – volume: 157
  start-page: 1724
  year: 2014
  end-page: 1734
  ident: CR22
  article-title: High-sensitivity measurements of multiple kinase activities in live single cells
  publication-title: Cell
  doi: 10.1016/j.cell.2014.04.039
– volume: 8
  year: 2017
  ident: CR33
  article-title: Optogenetic control of cellular forces and mechanotransduction
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms14396
– volume: 8
  start-page: e82693
  year: 2013
  ident: CR37
  article-title: Fluorescence imaging-based high-throughput screening of fast- and slow-cycling LOV proteins
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0082693
– volume: 85
  start-page: 179
  year: 2009
  end-page: 190
  ident: CR28
  article-title: Involvement of inflammatory mediators in neuropathic pain caused by vincristine
  publication-title: Int Rev. Neurobiol.
  doi: 10.1016/S0074-7742(09)85014-9
– volume: 276
  start-page: 36493
  year: 2001
  end-page: 36500
  ident: CR13
  article-title: The photocycle of a flavin-binding domain of the blue light photoreceptor phototropin
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M103114200
– volume: 6
  start-page: 100034
  year: 2019
  ident: CR26
  article-title: Alterations in brain neurocircuitry following treatment with the chemotherapeutic agent paclitaxel in rats
  publication-title: Neurobiol. Pain.
  doi: 10.1016/j.ynpai.2019.100034
– volume: 7
  year: 2017
  ident: CR40
  article-title: ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-017-07002-4
– volume: 19
  start-page: 507
  year: 2012
  end-page: 517
  ident: CR8
  article-title: Designing photoswitchable peptides using the AsLOV2 domain
  publication-title: Chem. Biol.
  doi: 10.1016/j.chembiol.2012.02.006
– volume: 168
  start-page: 159
  year: 2017
  end-page: 171.e14
  ident: CR31
  article-title: Spatiotemporal control of intracellular phase transitions using light-activated optoDroplets
  publication-title: Cell
  doi: 10.1016/j.cell.2016.11.054
– volume: 306
  start-page: 1370
  year: 2004
  end-page: 1373
  ident: CR11
  article-title: Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
  publication-title: Science
  doi: 10.1126/science.1102506
– volume: 45
  start-page: 6267
  year: 2006
  end-page: 6271
  ident: CR18
  article-title: Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer
  publication-title: Biochemistry
  doi: 10.1021/bi060093i
– volume: 26
  start-page: 1035
  year: 2008
  end-page: 1040
  ident: CR43
  article-title: Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1493
– volume: 7
  start-page: 3925
  year: 1968
  end-page: 3935
  ident: CR10
  article-title: Dimensions of protein random coils
  publication-title: Biochemistry
  doi: 10.1021/bi00851a021
– volume: 5463
  start-page: 97
  year: 2004
  end-page: 104
  ident: CR38
  article-title: Low-temperature and time-resolved spectroscopic characterization of the LOV2 domain of Avena sativa phototropin 1
  publication-title: Proc. SPIE
  doi: 10.1117/12.548054
– volume: 159
  start-page: 849
  year: 2018
  end-page: 863
  ident: CR27
  article-title: Disruption of nNOS-NOS1AP protein-protein interactions suppresses neuropathic pain in mice
  publication-title: Pain
  doi: 10.1097/j.pain.0000000000001152
– volume: 301
  start-page: 1541
  year: 2003
  end-page: 1544
  ident: CR21
  article-title: Structural basis of a phototropin light switch
  publication-title: Science
  doi: 10.1126/science.1086810
– volume: 7
  start-page: 3472
  year: 2016
  end-page: 3476
  ident: CR12
  article-title: Unfolding of the C-terminal Jα helix in the LOV2 photoreceptor domain observed by time-resolved vibrational spectroscopy
  publication-title: J. Phys. Chem. Lett.
  doi: 10.1021/acs.jpclett.6b01484
– volume: 10
  start-page: 240
  year: 2020
  end-page: 253.e6
  ident: CR5
  article-title: A live-cell screen for altered Erk dynamics reveals principles of proliferative control
  publication-title: Cell Syst.
  doi: 10.1016/j.cels.2020.02.005
– volume: 11
  year: 2020
  ident: CR19
  article-title: Optogenetic control of protein binding using light-switchable nanobodies
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-17836-8
– volume: 44
  start-page: 7085
  year: 2005
  end-page: 7094
  ident: CR50
  article-title: Reversible photobleaching of enhanced green fluorescent proteins
  publication-title: Biochemistry
  doi: 10.1021/bi047881x
– volume: 7
  year: 2016
  ident: CR9
  article-title: Optogenetic control of nuclear protein export
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms10624
– volume: 9
  year: 2018
  ident: CR32
  article-title: Guided morphogenesis through optogenetic activation of Rho signalling during early
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-018-04754-z
– volume: 9
  start-page: e87074
  year: 2014
  ident: CR17
  article-title: Factors that control the chemistry of the LOV domain photocycle
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0087074
– ident: CR47
– ident: CR14
– volume: 419
  start-page: 61
  year: 2012
  end-page: 74
  ident: CR15
  article-title: The amino-terminal helix modulates light-activated conformational changes in AsLOV2
  publication-title: J. Mol. Biol.
  doi: 10.1016/j.jmb.2012.02.037
– ident: CR2
– volume: 16
  start-page: 277
  year: 2019
  end-page: 278
  ident: CR48
  article-title: FPbase: a community-editable fluorescent protein database
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0352-8
– volume: 87
  start-page: 534
  year: 2011
  end-page: 541
  ident: CR39
  article-title: The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy
  publication-title: Photochem Photobiol.
  doi: 10.1111/j.1751-1097.2011.00903.x
– volume: 33
  start-page: 8185
  year: 2013
  end-page: 8201
  ident: CR41
  article-title: The nNOS-p38MAPK pathway is mediated by NOS1AP during neuronal death
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.4578-12.2013
– volume: 29
  start-page: 202
  year: 2019
  end-page: 216.e7
  ident: CR42
  article-title: Filopodome mapping identifies p130Cas as a mechanosensitive regulator of filopodia stability
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2018.11.053
– volume: 26
  start-page: 741
  year: 2016
  end-page: 755
  ident: CR45
  article-title: Interaction of NOS1AP with the NOS-I PDZ domain: Implications for schizophrenia-related alterations in dendritic morphology
  publication-title: Eur. Neuropsychopharmacol.
  doi: 10.1016/j.euroneuro.2016.01.008
– volume: 2
  start-page: 18
  year: 2015
  ident: CR35
  article-title: LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling
  publication-title: Front Mol. Biosci.
  doi: 10.3389/fmolb.2015.00018
– volume: 89
  start-page: 402
  year: 2005
  end-page: 412
  ident: CR16
  article-title: The phot LOV2 domain and its interaction with LOV1
  publication-title: Biophys. J.
  doi: 10.1529/biophysj.104.058230
– volume: 13
  start-page: 755
  year: 2016
  end-page: 758
  ident: CR25
  article-title: LOVTRAP: an optogenetic system for photoinduced protein dissociation
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3926
– volume: 2
  start-page: 905
  year: 2005
  end-page: 909
  ident: CR49
  article-title: A guide to choosing fluorescent proteins
  publication-title: Nat. Methods
  doi: 10.1038/nmeth819
– volume: 16
  start-page: 1029
  year: 2016
  end-page: 1036
  ident: CR36
  article-title: FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0541-5
– volume: 118
  start-page: 10659
  year: 2018
  end-page: 10709
  ident: CR1
  article-title: Blue-Light Receptors for Optogenetics
  publication-title: Chem. Rev.
  doi: 10.1021/acs.chemrev.8b00163
– volume: 27
  start-page: 1442
  year: 2016
  end-page: 1450
  ident: CR30
  article-title: Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration
  publication-title: Mol. Biol. Cell.
  doi: 10.1091/mbc.E15-12-0832
– volume: 216
  start-page: 25
  year: 2017
  end-page: 28
  ident: CR4
  article-title: Interrogating cellular perception and decision making with optogenetic tools
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201612094
– volume: 168
  start-page: 117
  year: 2005
  end-page: 126
  ident: CR51
  article-title: The PSD95-nNOS interface: a target for inhibition of excitotoxic p38 stress-activated protein kinase activation and cell death
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200407024
– volume: 5
  start-page: 827
  year: 2009
  end-page: 834
  ident: CR23
  article-title: Mechanism-based tuning of a LOV domain photoreceptor
  publication-title: Nat. Chem. Biol.
  doi: 10.1038/nchembio.210
– volume: 9
  start-page: 379
  year: 2012
  end-page: 384
  ident: CR24
  article-title: TULIPs: tunable, light-controlled interacting protein tags for cell biology
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1904
– volume: 466
  start-page: 263
  year: 2010
  end-page: 266
  ident: CR44
  article-title: Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
  publication-title: Nature
  doi: 10.1038/nature09198
– volume: 8
  year: 2017
  ident: CR6
  article-title: A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms15017
– volume: 461
  start-page: 104
  year: 2009
  end-page: 108
  ident: CR29
  article-title: A genetically encoded photoactivatable Rac controls the motility of living cells
  publication-title: Nature
  doi: 10.1038/nature08241
– volume: 1636
  start-page: 3
  year: 2017
  end-page: 20
  ident: CR34
  article-title: Optogenetic control of Ras/Erk signaling using the Phy-PIF system
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-7154-1_1
– ident: CR52
– volume: 94
  start-page: 37
  year: 2017
  end-page: 47.e5
  ident: CR7
  article-title: Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor
  publication-title: Neuron
  doi: 10.1016/j.neuron.2017.02.036
– volume: 10
  start-page: 436
  year: 2007
  end-page: 443
  ident: CR46
  article-title: Rho mediates calcium-dependent activation of p38alpha and subsequent excitotoxic cell death
  publication-title: Nat. Neurosci.
  doi: 10.1038/nn1869
– volume: 105
  start-page: 1027
  year: 2013
  end-page: 1036
  ident: CR3
  article-title: Investigating models of protein function and allostery with a widespread mutational analysis of a light-activated protein
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2013.07.010
– ident: CR20
– volume: 8
  year: 2017
  ident: 18816_CR6
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms15017
– volume: 45
  start-page: 6267
  year: 2006
  ident: 18816_CR18
  publication-title: Biochemistry
  doi: 10.1021/bi060093i
– volume: 29
  start-page: 202
  year: 2019
  ident: 18816_CR42
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2018.11.053
– volume: 157
  start-page: 1724
  year: 2014
  ident: 18816_CR22
  publication-title: Cell
  doi: 10.1016/j.cell.2014.04.039
– ident: 18816_CR52
  doi: 10.5281/zenodo.3882572
– ident: 18816_CR2
  doi: 10.1523/ENEURO.0386-19.2019
– volume: 94
  start-page: 37
  year: 2017
  ident: 18816_CR7
  publication-title: Neuron
  doi: 10.1016/j.neuron.2017.02.036
– volume: 6
  start-page: 100034
  year: 2019
  ident: 18816_CR26
  publication-title: Neurobiol. Pain.
  doi: 10.1016/j.ynpai.2019.100034
– volume: 44
  start-page: 7085
  year: 2005
  ident: 18816_CR50
  publication-title: Biochemistry
  doi: 10.1021/bi047881x
– volume: 276
  start-page: 36493
  year: 2001
  ident: 18816_CR13
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M103114200
– volume: 1636
  start-page: 3
  year: 2017
  ident: 18816_CR34
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-7154-1_1
– volume: 118
  start-page: 10659
  year: 2018
  ident: 18816_CR1
  publication-title: Chem. Rev.
  doi: 10.1021/acs.chemrev.8b00163
– volume: 7
  start-page: 3472
  year: 2016
  ident: 18816_CR12
  publication-title: J. Phys. Chem. Lett.
  doi: 10.1021/acs.jpclett.6b01484
– volume: 13
  start-page: 755
  year: 2016
  ident: 18816_CR25
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3926
– volume: 159
  start-page: 849
  year: 2018
  ident: 18816_CR27
  publication-title: Pain
  doi: 10.1097/j.pain.0000000000001152
– volume: 26
  start-page: 1035
  year: 2008
  ident: 18816_CR43
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1493
– volume: 26
  start-page: 741
  year: 2016
  ident: 18816_CR45
  publication-title: Eur. Neuropsychopharmacol.
  doi: 10.1016/j.euroneuro.2016.01.008
– ident: 18816_CR47
  doi: 10.21203/rs.3.pex-1131/v1
– volume: 7
  year: 2016
  ident: 18816_CR9
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms10624
– volume: 87
  start-page: 534
  year: 2011
  ident: 18816_CR39
  publication-title: Photochem Photobiol.
  doi: 10.1111/j.1751-1097.2011.00903.x
– volume: 19
  start-page: 507
  year: 2012
  ident: 18816_CR8
  publication-title: Chem. Biol.
  doi: 10.1016/j.chembiol.2012.02.006
– volume: 306
  start-page: 1370
  year: 2004
  ident: 18816_CR11
  publication-title: Science
  doi: 10.1126/science.1102506
– volume: 9
  year: 2018
  ident: 18816_CR32
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-018-04754-z
– volume: 168
  start-page: 117
  year: 2005
  ident: 18816_CR51
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200407024
– volume: 466
  start-page: 263
  year: 2010
  ident: 18816_CR44
  publication-title: Nature
  doi: 10.1038/nature09198
– volume: 419
  start-page: 61
  year: 2012
  ident: 18816_CR15
  publication-title: J. Mol. Biol.
  doi: 10.1016/j.jmb.2012.02.037
– volume: 27
  start-page: 1442
  year: 2016
  ident: 18816_CR30
  publication-title: Mol. Biol. Cell.
  doi: 10.1091/mbc.E15-12-0832
– volume: 7
  year: 2017
  ident: 18816_CR40
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-017-07002-4
– volume: 9
  start-page: e87074
  year: 2014
  ident: 18816_CR17
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0087074
– volume: 216
  start-page: 25
  year: 2017
  ident: 18816_CR4
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201612094
– volume: 8
  start-page: e82693
  year: 2013
  ident: 18816_CR37
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0082693
– volume: 16
  start-page: 277
  year: 2019
  ident: 18816_CR48
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0352-8
– volume: 85
  start-page: 179
  year: 2009
  ident: 18816_CR28
  publication-title: Int Rev. Neurobiol.
  doi: 10.1016/S0074-7742(09)85014-9
– volume: 16
  start-page: 1029
  year: 2016
  ident: 18816_CR36
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0541-5
– volume: 10
  start-page: 436
  year: 2007
  ident: 18816_CR46
  publication-title: Nat. Neurosci.
  doi: 10.1038/nn1869
– volume: 10
  start-page: 240
  year: 2020
  ident: 18816_CR5
  publication-title: Cell Syst.
  doi: 10.1016/j.cels.2020.02.005
– volume: 2
  start-page: 905
  year: 2005
  ident: 18816_CR49
  publication-title: Nat. Methods
  doi: 10.1038/nmeth819
– volume: 11
  year: 2020
  ident: 18816_CR19
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-17836-8
– ident: 18816_CR20
  doi: 10.1523/ENEURO.0303-19.2019
– volume: 7
  start-page: 3925
  year: 1968
  ident: 18816_CR10
  publication-title: Biochemistry
  doi: 10.1021/bi00851a021
– volume: 8
  year: 2017
  ident: 18816_CR33
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms14396
– volume: 5
  start-page: 827
  year: 2009
  ident: 18816_CR23
  publication-title: Nat. Chem. Biol.
  doi: 10.1038/nchembio.210
– volume: 301
  start-page: 1541
  year: 2003
  ident: 18816_CR21
  publication-title: Science
  doi: 10.1126/science.1086810
– volume: 105
  start-page: 1027
  year: 2013
  ident: 18816_CR3
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2013.07.010
– volume: 9
  start-page: 379
  year: 2012
  ident: 18816_CR24
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1904
– volume: 461
  start-page: 104
  year: 2009
  ident: 18816_CR29
  publication-title: Nature
  doi: 10.1038/nature08241
– ident: 18816_CR14
  doi: 10.1021/acschembio.0c00543
– volume: 168
  start-page: 159
  year: 2017
  ident: 18816_CR31
  publication-title: Cell
  doi: 10.1016/j.cell.2016.11.054
– volume: 89
  start-page: 402
  year: 2005
  ident: 18816_CR16
  publication-title: Biophys. J.
  doi: 10.1529/biophysj.104.058230
– volume: 5463
  start-page: 97
  year: 2004
  ident: 18816_CR38
  publication-title: Proc. SPIE
  doi: 10.1117/12.548054
– volume: 33
  start-page: 8185
  year: 2013
  ident: 18816_CR41
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.4578-12.2013
– volume: 2
  start-page: 18
  year: 2015
  ident: 18816_CR35
  publication-title: Front Mol. Biosci.
  doi: 10.3389/fmolb.2015.00018
SSID ssj0000391844
Score 2.3462107
Snippet Engineered light-dependent switches provide uniquely powerful opportunities to investigate and control cell regulatory mechanisms. Existing tools offer high...
Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use...
SourceID doaj
unpaywall
pubmedcentral
proquest
pubmed
crossref
springer
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 5107
SubjectTerms 13/95
14
14/63
631/1647/2253
631/61/338/469
631/92/552
96/10
96/33
96/35
96/95
Animals
Energy Transfer
Female
Fluorescence Resonance Energy Transfer - methods
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HEK293 Cells
Hippocampus - cytology
Humanities and Social Sciences
Humans
Light
Male
MAP Kinase Signaling System
Molecular Imaging - methods
multidisciplinary
Neurons
Optogenetics - methods
Phototropins - analysis
Phototropins - genetics
Phototropins - metabolism
Rats
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Science
Science (multidisciplinary)
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQJQQcEG9CARmJG7Vqx05iHwFRVYjHhaLeLD9hpSVZbbKq-u87drLRrkCFA1cnUeKZbzzjeOYbhF5TaQ2zjBNhrCAiMkmMoYZYiJ4hOvXR1ql2-POX-vRMfDyvzndafaWcsJEeeBTccfSOO0ljzWtwnqaxkgfATG1pbcqKZ_ZSKtXOZiqvwVzB1kVMVTKUy-Ne5DUh7ZaYlKwmcs8TZcL-P0WZvydLziemd9CtTbsylxdmudxxSif30N0pmsRvx1ncRzdC-wDdHPtLXj5EP9LP-cSoEXDINX54yHFqWOM-Ja6nRsw9Nq3Hv7Jpr3u8aDGMb3B_sRhymiXuIv709XtJkr_zuFsNHWAulT5ik4pP0lOP0NnJh2_vT8nUWYE4cPAD8c57aXgQIjYxkQA2EGWAK7OydN5aGSxtXOVAviwyByIHq1Y05U05WBCM54_RQdu14SnCClQTWLQuVrUI3JvoQyiNko6xwGlZILaVsnYT7XjqfrHU-fibSz1qRoNmdNaMlgV6Mz-zGkk3rr37XVLefGcizM4DACM9wUj_DUYFerVVvQYDS6cmpg3dptcQ_yhVSfDqBXoyQmF-FeeJ8VCpAjV7INn7lv0r7eJnJvFuKgEbG1Ggoy2c9LR69NfO9WiG3D-I5tn_EM0hul1ms6GEqufoYFhvwguIxAb7MhvdFZJiLnI
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: HAS SpringerNature Open Access 2022
  dbid: AAJSJ
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB6VrRDlgHg3vGQkbmxEEjuJc1wQVbXicYCi3iw_y0pLstpkVfXfM3ayEVFRBdfETmzPjOezPfMZ4E3ClUxVSmMmFYuZS3ksZSJjhegZ0alxqvC5w5-_FKdnbHmenx_AfJ8LMzm_D9TdLQvG7Jc5KedpEfNbcMhRMfkMDheL5bfluKfi2c45Y0NuDFZ_d73yxP8Emv6_YcvrIZLjOelduLOrN_LqUq7Xf7iik_twb8CQZNEL_QEc2Poh3O5vlbx6BBd-S97zaFhiQ2Yf6QI6tVvS-nB1f_1yS2RtyK9g0NuWrGqCz3ekvVx1IbiSNI58-voji72XM6TZdA1qmk94JNKnnPhaj-Hs5OP3D6fxcJ9CrNGtd7HRxnBJLWOudJ76r0RsgQ5M8UwbpbhVSalznaFZulQrTtGWq8RHS2mcBqShT2BWN7U9BlIlrrCpU9rlBbPUSGeszWTFdZpammQRpPtRFnogG_d3XqxFOPSmXPSSESgZESQjeARvxzqbnmrjxtLvvfDGkp4mOzxA7RGD1QlnNNUc20oLRF6yxD5ZnHAKlRQSu5lH8HoveoFm5c9KZG2bXSsQ9VRVztGXR_C0V4XxV5R6nsOqiqCcKMmkLdM39epnoO4uc4bLGRbBfK9OYpgz2hv7Oh9V7h-G5tn_ff05HGXBQJI4qV7ArNvu7EtEWp16NRjYb8CKIUg
  priority: 102
  providerName: Springer Nature
– databaseName: Unpaywall
  dbid: UNPAY
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELbKVgg48H6El4zEjWZrx07iHAuiqhAUDhTKyfKzrFiS1Sarqvx6xk6yYqGq6NWxFY89nvkSz3yD0EsitKKaspQrzVPuqUiVIirVgJ4BnVqvi5A7_OGwODji747z4y1UjLkwMWg_UlpGMz1Gh-22PB7p8LFDhaBFKqYL66-g7SIHDD5B20eHn_a-hUpyhNMUvDIbMmQIE-cM3vBCkaz_PIT5b6Dk-rb0Brq2qhfq7FTN5384pP1b6OsoSh-H8mO66vTU_PqL5fHyst5GNweMivf6nnfQlqvvoqt91cqze-gk_PIPPB0Ou5g5iLuIft0StyEcPpR3brGqLf4ZDcayxbMaQ_sKt6ezLgZv4sbj9x-_ZGnwohY3i64BTQ4JlViFlJYw6j462n_7-c1BOtRrSA3Ahi61xlqhmOPclz5QC5aAXcBBapEZq7VwmpQmNxkce0-NFgxsRUVCNJYBM6Mse4AmdVO7RwhXxBeOem18XnDHrPLWuUxVwlDqGMkSRMf9k2YgMw81NeYyXqozIftFlLCIMi6iFAl6tR6z6Kk8Luz9OqjFumeg4Y4NzfJEDlslvTXMCJgrKwDZqRJkcmDQCk0KBWLmCXoxKpWEYxvuYlTtmlUrAVVVVS4AKyToYa9k61cxFngUqypB5Yb6bcxl80k9-x6pwcucw-cST9DOqKhysEnthbLurJX5P5bm8eW6P0HXs6jLJCXVUzTpliv3DJBcp58Px_Y3HcpD2A
  priority: 102
  providerName: Unpaywall
Title Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators
URI https://link.springer.com/article/10.1038/s41467-020-18816-8
https://www.ncbi.nlm.nih.gov/pubmed/33037199
https://www.proquest.com/docview/2449958067
https://pubmed.ncbi.nlm.nih.gov/PMC7547724
https://www.nature.com/articles/s41467-020-18816-8.pdf
https://doaj.org/article/fdc3c80f636643a7b83e6386b06a2535
UnpaywallVersion publishedVersion
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAFT
  databaseName: Open Access Digital Library
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: KQ8
  dateStart: 20150101
  isFulltext: true
  titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html
  providerName: Colorado Alliance of Research Libraries
– providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals [Accès libre]
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: DOA
  dateStart: 20150101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVEBS
  databaseName: Inspec with Full Text
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: ADMLS
  dateStart: 20121101
  isFulltext: true
  titleUrlDefault: https://www.ebsco.com/products/research-databases/inspec-full-text
  providerName: EBSCOhost
– providerCode: PRVBFR
  databaseName: Free Medical Journals
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: DIK
  dateStart: 20100101
  isFulltext: true
  titleUrlDefault: http://www.freemedicaljournals.com
  providerName: Flying Publisher
– providerCode: PRVHPJ
  databaseName: ROAD: Directory of Open Access Scholarly Resources
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: M~E
  dateStart: 20100101
  isFulltext: true
  titleUrlDefault: https://road.issn.org
  providerName: ISSN International Centre
– providerCode: PRVAQN
  databaseName: PubMed Central Journals
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: RPM
  dateStart: 20120101
  isFulltext: true
  titleUrlDefault: https://www.ncbi.nlm.nih.gov/pmc/
  providerName: National Library of Medicine
– providerCode: PRVAQT
  databaseName: Nature Free
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: NAO
  dateStart: 20101201
  isFulltext: true
  titleUrlDefault: https://www.nature.com/siteindex/index.html
  providerName: Nature Publishing
– providerCode: PRVPQU
  databaseName: Health & Medical Collection
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: 7X7
  dateStart: 20100101
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/healthcomplete
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Proquest Central
  customDbUrl: http://www.proquest.com/pqcentral?accountid=15518
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: BENPR
  dateStart: 20100101
  isFulltext: true
  titleUrlDefault: https://www.proquest.com/central
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: ProQuest Technology Collection
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: 8FG
  dateStart: 20100401
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/technologycollection1
  providerName: ProQuest
– providerCode: PRVFZP
  databaseName: Scholars Portal Journals: Open Access
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 20250131
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: M48
  dateStart: 20101001
  isFulltext: true
  titleUrlDefault: http://journals.scholarsportal.info
  providerName: Scholars Portal
– providerCode: PRVAVX
  databaseName: HAS SpringerNature Open Access 2022
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: AAJSJ
  dateStart: 20101201
  isFulltext: true
  titleUrlDefault: https://www.springernature.com
  providerName: Springer Nature
– providerCode: PRVAVX
  databaseName: Springer Nature OA Free Journals
  customDbUrl:
  eissn: 2041-1723
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0000391844
  issn: 2041-1723
  databaseCode: C6C
  dateStart: 20101201
  isFulltext: true
  titleUrlDefault: http://www.springeropen.com/
  providerName: Springer Nature
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Zj9MwELb2EAIeEDfhqIzEG2uI4xzOA0LdasuqYssKKOo-Rb6yVCpJSVIt_feMnbSiolrBSyLZueyZz_M59swg9MrnUlBJGQmFDEmYU06E8AWRwJ6BnepcxtZ3-Gwcn07C0TSa7qF1uqOuA-udUzubT2pSzd_8-rl6D4B_17qM87d16OBuJ0KUcxoTvo8OwVKlNpXDWUf33cjMUpjQ2IXmwA8pAdvNOj-a3Y_ZslUupP8uHvr3dsrNmuptdHNZLMTqSsznf5it4V10p-ObuN8qyD20Z4r76EabgXL1AF3a3_c25obBxnkB4sYxWVPh2m5tt6maaywKjX848Fc1nhUYype4vpo1biMmLnP88dO3gFiLqHG5aErQSusciYV1T7F3PUST4cnXwSnpci8QBRSgIVppzQUzYZgnuQ0TmAAPAWMneaC0lNxIP1GRCgDCOVWSM8B96tudVQqGDKHZI3RQlIV5gnDq57GhuVR5FIeGaZFrYwKRckWpYX7gIbru5Ux1gcltfox55hbIGc9ayWQgmcxJJuMeer25Z9GG5bj26mMrvM2VNqS2Kyiry6xDaJZrxRSHb2UxsDSRQJsMDE6x9GMBzYw89HIt-gwgaNdVRGHKZZ0BQ0rTiIPd99DjVhU2r2LMxkRMUw8lW0qy9S3bNcXsuwvznUQhTH1CDx2t1Slbw-Path5tVO4fuubpf3XkM3QrcPjwiZ8-RwdNtTQvgJQ1sof2k2kCRz780EOH_f7oywjOxyfj889QOogHPfe7o-cQCTWT8Xn_4jfCOzfT
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB6VItRyQLwbnkbixkYksZM4R1hRLbAtlxb1ZvkJKy3JapNV1X_P2MlGrIoquCZ2YntmPJ_tmc8AbxOuZKpSGjOpWMxcymMpExkrRM-ITo1Thc8dPjktZufsy0V-sQeTbS7Mzvl9oO5uWTBmv8xJOU-LmN-C2xxXdJ4pf1pMxx0Vz3XOGRsyY7Dy--tVd7xPIOn_G7K8HiA5npLehYNNvZJXl3K5_MMRHd-HewOCJB96kT-APVs_hDv9nZJXj-CH35D3LBqW2JDXR7qATe2atD5Y3V--3BJZG_IrmPO6JYua4PMNaS8XXQitJI0j82_fs9j7OEOaVdegnvl0RyJ9womv9RjOjz-dTWfxcJtCrNGpd7HRxnBJLWOudJ74r0Rkge5L8UwbpbhVSalznaFRulQrTtGSq8THSmmcBKShT2C_bmp7BKRKXGFTp7TLC2apkc5Ym8mK6zS1NMkiSLejLPRANe5vvFiKcORNueglI1AyIkhG8AjejXVWPdHGjaU_euGNJT1JdniAuiMGmxPOaKo5tpUWiLtkiX2yON0UKikkdjOP4M1W9AKNyp-UyNo2m1Yg5qmqnKMnj-Bprwrjryj1LIdVFUG5oyQ7bdl9Uy9-BuLuMme4mGERTLbqJIYZo72xr5NR5f5haJ7939dfw8Hs7GQu5p9Pvz6HwywYSxIn1QvY79Yb-xIxV6deBVP7DZh2ItM
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Zb9QwEB6VVlwPiLOE00i80ZQkdhLncTlWZSkFCYr6ZvlsV1qS1Sarqv-esTcbsaKq4DWJHTsz4_kcz3wD8DrhSqYqpTGTisXMpTyWMpGxQvSM6NQ4Vfjc4S9HxcExm5zkJ1tQrHNhQtB-oLQMy_Q6Ouxty4JJ-81OynlaxHx_btw12OElLVCbd0ajyffJ8HfF855zxvosmYTySzrY8ESBsP8ylPl3sORwYnobbi7rubw4l7PZH05pfBfu9GiSjFbjvwdbtr4P11f1JS8ewKn_Oe8ZNSyxIcePdAGn2gVpfeC6L8TcElkb8iuY9qIl05rg9SVpz6ddCLMkjSOHX39msfd3hjTzrkGd86mPRPrkE9_qIRyPP_54fxD3lRVijQ6-i402hktqGXOl8ySAJaIMdGWKZ9ooxa1KSp3rDA3UpVpxilZdJT5uSuOCIA19BNt1U9vHQKrEFTZ1Sru8YJYa6Yy1may4TlNLkyyCdP2Vhe5px331i5kIx9-Ui5VkBEpGBMkIHsGboc18Rbpx5dPvvPCGJz1hdrjQLE5Fr0DCGU01x7HSAjGYLHFOFpeeQiWFxGnmEbxai16ggflTE1nbZtkKxD9VlXP06hHsrlRheBWlnvGwqiIoN5RkYyybd-rpWSDxLnOGGxsWwd5anUS_erRXznVvULl_-DRP_q_3l3Dj24exOPx09Pkp3MqCrSRxUj2D7W6xtM8RfnXqRW9rvwE1uim6
linkToUnpaywall http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELbKVgg48H6El4zEjWZrx07iHAuiqhAUDhTKyfKzrFiS1Sarqvx6xk6yYqGq6NWxFY89nvkSz3yD0EsitKKaspQrzVPuqUiVIirVgJ4BnVqvi5A7_OGwODji747z4y1UjLkwMWg_UlpGMz1Gh-22PB7p8LFDhaBFKqYL66-g7SIHDD5B20eHn_a-hUpyhNMUvDIbMmQIE-cM3vBCkaz_PIT5b6Dk-rb0Brq2qhfq7FTN5384pP1b6OsoSh-H8mO66vTU_PqL5fHyst5GNweMivf6nnfQlqvvoqt91cqze-gk_PIPPB0Ou5g5iLuIft0StyEcPpR3brGqLf4ZDcayxbMaQ_sKt6ezLgZv4sbj9x-_ZGnwohY3i64BTQ4JlViFlJYw6j462n_7-c1BOtRrSA3Ahi61xlqhmOPclz5QC5aAXcBBapEZq7VwmpQmNxkce0-NFgxsRUVCNJYBM6Mse4AmdVO7RwhXxBeOem18XnDHrPLWuUxVwlDqGMkSRMf9k2YgMw81NeYyXqozIftFlLCIMi6iFAl6tR6z6Kk8Luz9OqjFumeg4Y4NzfJEDlslvTXMCJgrKwDZqRJkcmDQCk0KBWLmCXoxKpWEYxvuYlTtmlUrAVVVVS4AKyToYa9k61cxFngUqypB5Yb6bcxl80k9-x6pwcucw-cST9DOqKhysEnthbLurJX5P5bm8eW6P0HXs6jLJCXVUzTpliv3DJBcp58Px_Y3HcpD2A
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Resonance+energy+transfer+sensitises+and+monitors+in+situ+switching+of+LOV2-based+optogenetic+actuators&rft.jtitle=Nature+communications&rft.au=Li%2C+Li-Li&rft.au=Klein%2C+Florence+M.&rft.au=Li+Greci%2C+Lorenzo&rft.au=Popinigis%2C+Arkadiusz&rft.date=2020-10-09&rft.issn=2041-1723&rft.eissn=2041-1723&rft.volume=11&rft.issue=1&rft_id=info:doi/10.1038%2Fs41467-020-18816-8&rft.externalDBID=n%2Fa&rft.externalDocID=10_1038_s41467_020_18816_8
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2041-1723&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2041-1723&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2041-1723&client=summon