Stable gene-silence of Kif2a synergistic with 5-fluorouracil suppresses oral tongue squamous cell carcinoma growth in vitro and in vivo

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 116; no. 1; pp. 49 - 54
• Main Authors: Wang, Cheng-Qin, Li, Yu-Jun, Wei, Zhi-Min, Zhu, Chang-Jun, Qu, Xun, Wei, Feng-Cai, Xing, Xiao-Ming, Yu, Wen-Juan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2013
• Subjects: Animals; Antimetabolites, Antineoplastic - pharmacology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - therapy; Cell Line, Tumor; Fluorouracil - pharmacology; Gene Silencing; Humans; Kinesin - genetics; Male; Mice; Mice, Nude; Plasmids; Repressor Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Small Interfering; Surgery; Tongue Neoplasms - genetics; Tongue Neoplasms - metabolism; Tongue Neoplasms - therapy; Transfection
• ISSN: 2212-4403; 2212-4411; 2212-4411
• DOI: 10.1016/j.oooo.2013.01.028

Squamous cell carcinoma of the oral tongue (SCCOT) is one of the most common malignant carcinomas in the head and neck. Recurrence and/or metastasis often results in failure of treatment and decreases the survival of the patients. The purpose of this study is to investigate the effect of gene-silence of Kif2a on SCCOT in viro and in vivo. Plasmid-mediated expression of Kif2a-siRNA (pGPU6/GFP/Kif2a) was employed to silence the expression of Kif2a in Tca8113 cells at both mRNA and protein levels. Tca8113 cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and growth of Tca8113 tumors was determined by intra-tumor injection of pGPU6/GFP/Kif2a in nude mice. Gene-silence of Kif2a suppressed Tca8113 cell proliferation. pGPU6/GFP/Kif2a synergized the tumor suppression effect of 5-Fluorouracil (5-Fu) on Tca8113 cells. Our data support that Kif2a is a potential molecular target for the therapeutics of recurrent and metastatic SCCOT.