Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents
Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or...
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| Published in | Scientific reports Vol. 6; no. 1; p. 33641 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
London
Nature Publishing Group UK
19.09.2016
Nature Publishing Group |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2045-2322 2045-2322 |
| DOI | 10.1038/srep33641 |
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| Abstract | Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or precipitating agents such as Polyethylene glycol (PEG) or PRotein Organic Solvent PRecipitation (PROSPR) have emerged. To evaluate the impact of these different methods on the resulting EV preparations, plasma EVs were isolated using SEC, PEG and PROSPR and their total protein content, NTA and Cryo-electron microscopy profiles and EV-markers were compared. Also, their effect on recipient cells was tested. Low protein content and Cryo-EM analysis showed that SEC removed most of the overabundant soluble plasma proteins, which were not removed using PEG and partially by PROSPR. Moreover, only SEC allowed the detection of the EV-markers CD9, CD63 and CD81, LGALS3BP and CD5L, suggesting a putative interference of the precipitating agents in the structure/composition of the EVs. Furthermore, PEG and PROSPR-based EV isolation resulted in reduced cell viability
in vitro
. These results stress that appropriate EV-isolation method should be considered depending on the forthcoming application of the purified EVs. |
|---|---|
| AbstractList | Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or precipitating agents such as Polyethylene glycol (PEG) or PRotein Organic Solvent PRecipitation (PROSPR) have emerged. To evaluate the impact of these different methods on the resulting EV preparations, plasma EVs were isolated using SEC, PEG and PROSPR, and their total protein content, NTA and Cryo-electron microscopy profiles, and EV-markers were compared. Also, their effect on recipient cells was tested. Low protein content and Cryo-EM analysis showed that SEC removed most of the overabundant soluble plasma proteins, which were not removed using PEG and partially by PROSPR. Moreover, only SEC allowed the detection of the EV-markers CD9, CD63 and CD81, LGALS3BP and CD5L, suggesting a putative interference of the precipitating agents in the structure/composition of the EVs. Furthermore, PEG and PROSPR-based EV isolation resulted in reduced cell viability in vitro. These results stress that appropriate EV-isolation method should be considered depending on the forthcoming application of the purified EVs. Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or precipitating agents such as Polyethylene glycol (PEG) or PRotein Organic Solvent PRecipitation (PROSPR) have emerged. To evaluate the impact of these different methods on the resulting EV preparations, plasma EVs were isolated using SEC, PEG and PROSPR and their total protein content, NTA and Cryo-electron microscopy profiles and EV-markers were compared. Also, their effect on recipient cells was tested. Low protein content and Cryo-EM analysis showed that SEC removed most of the overabundant soluble plasma proteins, which were not removed using PEG and partially by PROSPR. Moreover, only SEC allowed the detection of the EV-markers CD9, CD63 and CD81, LGALS3BP and CD5L, suggesting a putative interference of the precipitating agents in the structure/composition of the EVs. Furthermore, PEG and PROSPR-based EV isolation resulted in reduced cell viability in vitro . These results stress that appropriate EV-isolation method should be considered depending on the forthcoming application of the purified EVs. |
| ArticleNumber | 33641 |
| Author | Carreras-Planella, Laura Gámez-Valero, Ana Borràs, Francesc E. Beyer, Katrin Monguió-Tortajada, Marta Franquesa, Marcel·la |
| Author_xml | – sequence: 1 givenname: Ana surname: Gámez-Valero fullname: Gámez-Valero, Ana organization: REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol, Can Ruti Campus, Department of Pathology, Hospital Universitari and Health Sciences Research Institute Germans Trias i Pujol, Universitat Autònoma de Barcelona – sequence: 2 givenname: Marta surname: Monguió-Tortajada fullname: Monguió-Tortajada, Marta organization: REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol, Can Ruti Campus – sequence: 3 givenname: Laura surname: Carreras-Planella fullname: Carreras-Planella, Laura organization: REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol, Can Ruti Campus – sequence: 4 givenname: Marcel·la surname: Franquesa fullname: Franquesa, Marcel·la organization: REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol, Can Ruti Campus – sequence: 5 givenname: Katrin surname: Beyer fullname: Beyer, Katrin organization: Department of Pathology, Hospital Universitari and Health Sciences Research Institute Germans Trias i Pujol, Universitat Autònoma de Barcelona – sequence: 6 givenname: Francesc E. surname: Borràs fullname: Borràs, Francesc E. organization: REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol, Can Ruti Campus, Nephrology Service, Germans Trias i Pujol University Hospital |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27640641$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1371/journal.pbio.1001450 10.1021/acsnano.5b08015 10.1038/srep14664 10.1016/j.ymeth.2015.03.009 10.1002/pmic.201300371 10.1016/j.compbiolchem.2013.04.005 10.1038/leu.2014.41 10.1590/1414-431X20132964 10.1016/j.thromres.2013.11.010 10.1111/j.1538-7836.2011.04610.x 10.3402/jev.v2i0.20360 10.1016/j.bbagen.2012.03.017 10.3402/jev.v4.27066 10.1038/srep23978 10.3389/fimmu.2015.00066 10.1093/nar/gkr828 10.1002/prca.201400114 10.3402/jev.v4.27378 10.3402/jev.v3.23262 10.3389/fimmu.2015.00006 10.1038/srep17319 10.1371/journal.pone.0145686 10.3402/jev.v4.27369 |
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| References | Lacroix, Judicone, Poncelet, Robert, Arnaud, Sampol, Dignat-George (CR23) 2012; 10 Yuana, Levels, Grootemaat, Sturk, Nieuwland (CR24) 2014; 3 CR18 Sáenz-Cuesta (CR10) 2015; 6 Boukouris, Mathivanan (CR7) 2015; 9 De Menezes-Neto (CR13) 2015; 4 CR14 Kalra (CR5) 2012; 10 Benito-Martin, Di Giannatale, Ceder, Peinado (CR1) 2015; 6 Gámez-Valero, Lozano-Ramos, Bancu, Lauzurica-Valdemoros, Borràs (CR15) 2015; 6 Witwer (CR19) 2013; 2 Vlassov, Magdaleno, Setterquist, Conrad (CR11) 2012; 1820 Kordelas (CR26) 2014; 28 Borges, Reis, Schor (CR2) 2013; 46 Fais (CR8) 2016; 10 Gallart-Palau (CR16) 2015; 5 György (CR21) 2014; 133 Zwicker, Lacroix, Dignat-george, Furie, Furie (CR20) 2012; 788 Böing, Pol, Der, Grootemaat (CR12) 2014; 1 Li (CR22) 2015; 87 Yáñez-Mó (CR3) 2015; 4 Mathivanan, Fahner, Reid, Simpson (CR4) 2012; 40 Rider, Hurwitz, Meckes (CR25) 2016; 6 Posokhov, Kyrychenko (CR17) 2013; 46 Wang, Sun (CR6) 2014; 14 Livshts (CR9) 2015; 5 H Kalra (BFsrep33641_CR5) 2012; 10 S Fais (BFsrep33641_CR8) 2016; 10 S Mathivanan (BFsrep33641_CR4) 2012; 40 KW Witwer (BFsrep33641_CR19) 2013; 2 A Vlassov V. (BFsrep33641_CR11) 2012; 1820 M Li (BFsrep33641_CR22) 2015; 87 MA Rider (BFsrep33641_CR25) 2016; 6 L Kordelas (BFsrep33641_CR26) 2014; 28 M Yáñez-Mó (BFsrep33641_CR3) 2015; 4 A De Menezes-Neto (BFsrep33641_CR13) 2015; 4 JI Zwicker (BFsrep33641_CR20) 2012; 788 M Sáenz-Cuesta (BFsrep33641_CR10) 2015; 6 A Gámez-Valero (BFsrep33641_CR15) 2015; 6 YO Posokhov (BFsrep33641_CR17) 2013; 46 D Wang (BFsrep33641_CR6) 2014; 14 BFsrep33641_CR18 Ma Livshts (BFsrep33641_CR9) 2015; 5 B György (BFsrep33641_CR21) 2014; 133 X Gallart-Palau (BFsrep33641_CR16) 2015; 5 A Benito-Martin (BFsrep33641_CR1) 2015; 6 S Boukouris (BFsrep33641_CR7) 2015; 9 BFsrep33641_CR14 R Lacroix (BFsrep33641_CR23) 2012; 10 Y Yuana (BFsrep33641_CR24) 2014; 3 FT Borges (BFsrep33641_CR2) 2013; 46 AN Böing (BFsrep33641_CR12) 2014; 1 25279113 - J Extracell Vesicles. 2014 Sep 08;3:null 24360116 - Thromb Res. 2014 Feb;133(2):285-92 24141609 - Braz J Med Biol Res. 2013 Oct;46(10):824-30 22130705 - Methods Mol Biol. 2012;788:127-39 25814440 - Methods. 2015 Oct 1;87:26-30 25688242 - Front Immunol. 2015 Jan 30;6:6 23764528 - Comput Biol Chem. 2013 Oct;46:23-31 25762995 - Front Immunol. 2015 Feb 13;6:50 26690353 - PLoS One. 2015 Dec 21;10(12):e0145686 26616523 - Sci Rep. 2015 Nov 30;5:17319 21989406 - Nucleic Acids Res. 2012 Jan;40(Database issue):D1241-4 23271954 - PLoS Biol. 2012;10(12):e1001450 26978483 - ACS Nano. 2016 Apr 26;10(4):3886-99 22212198 - J Thromb Haemost. 2012 Mar;10(3):437-46 27068479 - Sci Rep. 2016 Apr 12;6:23978 26025625 - J Extracell Vesicles. 2015 May 28;4:27369 25759690 - Front Immunol. 2015 Feb 24;6:66 26419333 - Sci Rep. 2015 Sep 30;5:14664 25018865 - J Extracell Vesicles. 2014 Jul 08;3:null 25979354 - J Extracell Vesicles. 2015 May 14;4:27066 24009894 - J Extracell Vesicles. 2013 May 27;2:null 22503788 - Biochim Biophys Acta. 2012 Jul;1820(7):940-8 25684126 - Proteomics Clin Appl. 2015 Apr;9(3-4):358-67 24962155 - Proteomics. 2014 Aug;14(16):1922-32 26154623 - J Extracell Vesicles. 2015 Jul 06;4:27378 24445866 - Leukemia. 2014 Apr;28(4):970-3 |
| References_xml | – volume: 10 start-page: e1001450 year: 2012 ident: CR5 article-title: Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation publication-title: Plos Biol doi: 10.1371/journal.pbio.1001450 – volume: 10 start-page: 3886 year: 2016 end-page: 3899 ident: CR8 article-title: Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine publication-title: ACS Nano doi: 10.1021/acsnano.5b08015 – ident: CR18 – volume: 5 start-page: 14664 year: 2015 ident: CR16 article-title: Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR) publication-title: Sci. Rep. doi: 10.1038/srep14664 – volume: 87 start-page: 26 year: 2015 end-page: 30 ident: CR22 article-title: An optimized procedure for exosome isolation and analysis using serum samples: Application to cancer biomarker discovery publication-title: Methods doi: 10.1016/j.ymeth.2015.03.009 – volume: 14 start-page: 1922 year: 2014 end-page: 1932 ident: CR6 article-title: Urinary extracellular microvesicles: Isolation methods and prospects for urinary proteome publication-title: Proteomics doi: 10.1002/pmic.201300371 – volume: 6 start-page: 50 year: 2015 ident: CR10 article-title: Methods for extracellular vesicles isolation in a hospital setting publication-title: Front. Immunol. – ident: CR14 – volume: 46 start-page: 23 year: 2013 end-page: 31 ident: CR17 article-title: Effect of acetone accumulation on structure and dynamics of lipid membranes studied by molecular dynamics simulations publication-title: Comput. Biol. Chem. doi: 10.1016/j.compbiolchem.2013.04.005 – volume: 28 start-page: 970 year: 2014 end-page: 973 ident: CR26 article-title: MSC-derived exosomes: a novel tool to treat therapy-refractory graft-versus-host disease publication-title: Leukemia doi: 10.1038/leu.2014.41 – volume: 46 start-page: 824 year: 2013 end-page: 830 ident: CR2 article-title: Extracellular vesicles: structure, function and potential clinical uses in renal diseases publication-title: Braz. J. Med. Biol. Res. doi: 10.1590/1414-431X20132964 – volume: 133 start-page: 285 year: 2014 end-page: 292 ident: CR21 article-title: Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube publication-title: Thromb. Res. doi: 10.1016/j.thromres.2013.11.010 – volume: 10 start-page: 437 year: 2012 end-page: 446 ident: CR23 article-title: Impact of pre-analytical parameters on the measureent of circulating microparticles: Towards standardization of protocol publication-title: J. Thromb. Haemost doi: 10.1111/j.1538-7836.2011.04610.x – volume: 2 start-page: 1 year: 2013 end-page: 25 ident: CR19 article-title: Standardization of sample collection, isolation and analysis methods in extracellular vesicle research publication-title: J. Extracell. vesicles doi: 10.3402/jev.v2i0.20360 – volume: 1820 start-page: 940 year: 2012 end-page: 948 ident: CR11 article-title: Exosomes: current knowledge of their composition, biological functions and diagnostic and therapeutic potentials publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbagen.2012.03.017 – volume: 4 start-page: 27066 year: 2015 ident: CR3 article-title: Biological properties of extracellular vesicles and their physiological functions publication-title: J. Extracell. vesicles doi: 10.3402/jev.v4.27066 – volume: 6 start-page: 23978 year: 2016 ident: CR25 article-title: ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles publication-title: Scientific Reports doi: 10.1038/srep23978 – volume: 6 start-page: 66 year: 2015 ident: CR1 article-title: The new deal: A potential role for secreted vesicles in innate immunity and tumor progression publication-title: Frontiers in Immunology doi: 10.3389/fimmu.2015.00066 – volume: 40 start-page: D1241 year: 2012 end-page: D1244 ident: CR4 article-title: ExoCarta 2012: database of exosomal proteins, RNA and lipids publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkr828 – volume: 9 start-page: 358 year: 2015 end-page: 367 ident: CR7 article-title: Exosomes in bodily fluids are a highly stable resource of disease biomarkers publication-title: Proteomics - Clinical Applications doi: 10.1002/prca.201400114 – volume: 4 start-page: 1 year: 2015 end-page: 14 ident: CR13 article-title: Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals publication-title: J. Extracell. Vesicles doi: 10.3402/jev.v4.27378 – volume: 788 start-page: 127 year: 2012 end-page: 139 ident: CR20 article-title: Platelets and Megakaryocytes publication-title: Methods – volume: 3 start-page: 1 year: 2014 end-page: 5 ident: CR24 article-title: Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation publication-title: J. Extracell. vesicles doi: 10.3402/jev.v3.23262 – volume: 1 start-page: 1 year: 2014 end-page: 11 ident: CR12 publication-title: Single-step isolation of extracellular vesicles by size-exclusion chromatography – volume: 6 start-page: 1 year: 2015 end-page: 10 ident: CR15 article-title: Urinary extracellular vesicles as source of biomarkers in kidney diseases publication-title: Front. Immunol. doi: 10.3389/fimmu.2015.00006 – volume: 5 start-page: 17319 year: 2015 ident: CR9 article-title: Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol publication-title: Sci. Rep. doi: 10.1038/srep17319 – volume: 6 start-page: 23978 year: 2016 ident: BFsrep33641_CR25 publication-title: Scientific Reports doi: 10.1038/srep23978 – volume: 1820 start-page: 940 year: 2012 ident: BFsrep33641_CR11 publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbagen.2012.03.017 – volume: 2 start-page: 1 year: 2013 ident: BFsrep33641_CR19 publication-title: J. Extracell. vesicles doi: 10.3402/jev.v2i0.20360 – volume: 5 start-page: 14664 year: 2015 ident: BFsrep33641_CR16 publication-title: Sci. Rep. doi: 10.1038/srep14664 – volume: 6 start-page: 66 year: 2015 ident: BFsrep33641_CR1 publication-title: Frontiers in Immunology doi: 10.3389/fimmu.2015.00066 – volume: 4 start-page: 27066 year: 2015 ident: BFsrep33641_CR3 publication-title: J. Extracell. vesicles doi: 10.3402/jev.v4.27066 – volume: 788 start-page: 127 year: 2012 ident: BFsrep33641_CR20 publication-title: Methods – volume: 4 start-page: 1 year: 2015 ident: BFsrep33641_CR13 publication-title: J. Extracell. Vesicles doi: 10.3402/jev.v4.27378 – volume: 9 start-page: 358 year: 2015 ident: BFsrep33641_CR7 publication-title: Proteomics - Clinical Applications doi: 10.1002/prca.201400114 – volume: 6 start-page: 50 year: 2015 ident: BFsrep33641_CR10 publication-title: Front. Immunol. – volume: 6 start-page: 1 year: 2015 ident: BFsrep33641_CR15 publication-title: Front. Immunol. doi: 10.3389/fimmu.2015.00006 – volume: 5 start-page: 17319 year: 2015 ident: BFsrep33641_CR9 publication-title: Sci. Rep. doi: 10.1038/srep17319 – volume: 46 start-page: 23 year: 2013 ident: BFsrep33641_CR17 publication-title: Comput. Biol. Chem. doi: 10.1016/j.compbiolchem.2013.04.005 – ident: BFsrep33641_CR18 doi: 10.1371/journal.pone.0145686 – volume: 1 start-page: 1 year: 2014 ident: BFsrep33641_CR12 publication-title: Single-step isolation of extracellular vesicles by size-exclusion chromatography – volume: 133 start-page: 285 year: 2014 ident: BFsrep33641_CR21 publication-title: Thromb. Res. doi: 10.1016/j.thromres.2013.11.010 – volume: 10 start-page: 437 year: 2012 ident: BFsrep33641_CR23 publication-title: J. Thromb. Haemost doi: 10.1111/j.1538-7836.2011.04610.x – volume: 40 start-page: D1241 year: 2012 ident: BFsrep33641_CR4 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkr828 – volume: 10 start-page: 3886 year: 2016 ident: BFsrep33641_CR8 publication-title: ACS Nano doi: 10.1021/acsnano.5b08015 – volume: 14 start-page: 1922 year: 2014 ident: BFsrep33641_CR6 publication-title: Proteomics doi: 10.1002/pmic.201300371 – volume: 3 start-page: 1 year: 2014 ident: BFsrep33641_CR24 publication-title: J. Extracell. vesicles doi: 10.3402/jev.v3.23262 – volume: 10 start-page: e1001450 year: 2012 ident: BFsrep33641_CR5 publication-title: Plos Biol doi: 10.1371/journal.pbio.1001450 – volume: 87 start-page: 26 year: 2015 ident: BFsrep33641_CR22 publication-title: Methods doi: 10.1016/j.ymeth.2015.03.009 – ident: BFsrep33641_CR14 doi: 10.3402/jev.v4.27369 – volume: 46 start-page: 824 year: 2013 ident: BFsrep33641_CR2 publication-title: Braz. J. Med. Biol. Res. doi: 10.1590/1414-431X20132964 – volume: 28 start-page: 970 year: 2014 ident: BFsrep33641_CR26 publication-title: Leukemia doi: 10.1038/leu.2014.41 – reference: 26616523 - Sci Rep. 2015 Nov 30;5:17319 – reference: 22503788 - Biochim Biophys Acta. 2012 Jul;1820(7):940-8 – reference: 25759690 - Front Immunol. 2015 Feb 24;6:66 – reference: 25814440 - Methods. 2015 Oct 1;87:26-30 – reference: 27068479 - Sci Rep. 2016 Apr 12;6:23978 – reference: 26154623 - J Extracell Vesicles. 2015 Jul 06;4:27378 – reference: 25018865 - J Extracell Vesicles. 2014 Jul 08;3:null – reference: 25762995 - Front Immunol. 2015 Feb 13;6:50 – reference: 25279113 - J Extracell Vesicles. 2014 Sep 08;3:null – reference: 24445866 - Leukemia. 2014 Apr;28(4):970-3 – reference: 26978483 - ACS Nano. 2016 Apr 26;10(4):3886-99 – reference: 22212198 - J Thromb Haemost. 2012 Mar;10(3):437-46 – reference: 26690353 - PLoS One. 2015 Dec 21;10(12):e0145686 – reference: 25688242 - Front Immunol. 2015 Jan 30;6:6 – reference: 26419333 - Sci Rep. 2015 Sep 30;5:14664 – reference: 22130705 - Methods Mol Biol. 2012;788:127-39 – reference: 23271954 - PLoS Biol. 2012;10(12):e1001450 – reference: 21989406 - Nucleic Acids Res. 2012 Jan;40(Database issue):D1241-4 – reference: 24009894 - J Extracell Vesicles. 2013 May 27;2:null – reference: 24962155 - Proteomics. 2014 Aug;14(16):1922-32 – reference: 25979354 - J Extracell Vesicles. 2015 May 14;4:27066 – reference: 23764528 - Comput Biol Chem. 2013 Oct;46:23-31 – reference: 24360116 - Thromb Res. 2014 Feb;133(2):285-92 – reference: 24141609 - Braz J Med Biol Res. 2013 Oct;46(10):824-30 – reference: 25684126 - Proteomics Clin Appl. 2015 Apr;9(3-4):358-67 – reference: 26025625 - J Extracell Vesicles. 2015 May 28;4:27369 |
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| Snippet | Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs... |
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| SubjectTerms | 631/80/313/2162 631/80/86/820 CD63 antigen CD81 antigen CD9 antigen Chromatography Electron microscopy Extracellular vesicles Humanities and Social Sciences multidisciplinary Plasma proteins Polyethylene glycol Proteins Science Ultracentrifugation |
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| Title | Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents |
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