Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy

Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob‐Ferreira ALB, Tanus‐Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP‐8, MMP‐9, TIMP‐2, and MPO decrease after periodontal therapy. J Clin Periodontol 2010; 37: 180–190. doi: 10.1111/j.1600‐051X.20...

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Published inJournal of clinical periodontology Vol. 37; no. 2; pp. 180 - 190
Main Authors Marcaccini, Andrea M., Meschiari, Cesar A., Zuardi, Leonardo R., De Sousa, Tiago Sampaio, Taba Jr, Mario, Teofilo, Juliana M., Jacob-Ferreira, Anna L.B., Tanus-Santos, Jose E., Novaes Jr, Arthur B., Gerlach, Raquel F.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.02.2010
Blackwell
Subjects
Adult
Biological and medical sciences
Case-Control Studies
chronic periodontitis
Chronic Periodontitis - enzymology
Chronic Periodontitis - immunology
Chronic Periodontitis - therapy
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
GCF
Gingiva - enzymology
gingival crevicular fluid
Gingival Crevicular Fluid - enzymology
Gingival Crevicular Fluid - immunology
Humans
Male
Matrix Metalloproteinase 8 - metabolism
Matrix Metalloproteinase 9 - metabolism
Medical sciences
Middle Aged
MMP-8
MMP-9
MPO
Non tumoral diseases
non-surgical therapy
Otorhinolaryngology. Stomatology
Peroxidase - metabolism
Reference Values
Statistics, Nonparametric
TIMP-1
TIMP-2
Tissue Inhibitor of Metalloproteinase-1 - metabolism
Tissue Inhibitor of Metalloproteinase-2 - metabolism
Treatment Outcome
TIMP-2
TIMP-1
Stomatology
Crevicular fluid
chronic periodontitis
Dentistry
GCF
MPO
non-surgical therapy
Chronic
Periodontal disease
Treatment
Periodontitis
Surgery
gingival crevicular fluid
MMP-8
MMP-9
Gingival fluid
Online AccessGet full text
ISSN0303-6979
1600-051X
1600-051X
DOI10.1111/j.1600-051X.2009.01512.x

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Abstract Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob‐Ferreira ALB, Tanus‐Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP‐8, MMP‐9, TIMP‐2, and MPO decrease after periodontal therapy. J Clin Periodontol 2010; 37: 180–190. doi: 10.1111/j.1600‐051X.2009.01512.x. Background: This study aimed at comparing the levels of matrix metalloproteinase (MMP)‐8, tissue Inhibitor of MMPs (TIMP)‐1 and TIMP‐2, Myeloperoxidase (MPO), and MMP‐9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non‐surgical therapy. Materials and Methods: GCF was collected from one site of 15 control subjects and 27 CP patients. MMP‐8, MMP‐9, TIMP‐1, and TIMP‐2 were determined by Enzyme‐linked immunoabsorbent assay; different forms of MMP‐9, by gelatin zymography; and MPO, colorimetrically. Results: At baseline, higher levels of MMP‐8, TIMP‐2, MPO, and the 87 kDa‐MMP‐9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP‐9 (180, 130, 92 kDa) or total MMP‐9 at baseline. No differences were observed in TIMP‐1 levels. In controls, decreased levels of TIMP‐2 and the higher molecular forms of MMP‐9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01). Conclusions: Higher levels of MMP‐8, TIMP‐2, MPO, and 87 kDa MMP‐9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.
AbstractList This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy.BACKGROUNDThis study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy.GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically.MATERIALS AND METHODSGCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically.At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01).RESULTSAt baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01).Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.CONCLUSIONSHigher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.
This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy. GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically. At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01). Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.
Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob‐Ferreira ALB, Tanus‐Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP‐8, MMP‐9, TIMP‐2, and MPO decrease after periodontal therapy. J Clin Periodontol 2010; 37: 180–190. doi: 10.1111/j.1600‐051X.2009.01512.x. Background: This study aimed at comparing the levels of matrix metalloproteinase (MMP)‐8, tissue Inhibitor of MMPs (TIMP)‐1 and TIMP‐2, Myeloperoxidase (MPO), and MMP‐9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non‐surgical therapy. Materials and Methods: GCF was collected from one site of 15 control subjects and 27 CP patients. MMP‐8, MMP‐9, TIMP‐1, and TIMP‐2 were determined by Enzyme‐linked immunoabsorbent assay; different forms of MMP‐9, by gelatin zymography; and MPO, colorimetrically. Results: At baseline, higher levels of MMP‐8, TIMP‐2, MPO, and the 87 kDa‐MMP‐9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP‐9 (180, 130, 92 kDa) or total MMP‐9 at baseline. No differences were observed in TIMP‐1 levels. In controls, decreased levels of TIMP‐2 and the higher molecular forms of MMP‐9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01). Conclusions: Higher levels of MMP‐8, TIMP‐2, MPO, and 87 kDa MMP‐9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.
Author Tanus-Santos, Jose E.
Zuardi, Leonardo R.
Teofilo, Juliana M.
Meschiari, Cesar A.
Novaes Jr, Arthur B.
De Sousa, Tiago Sampaio
Jacob-Ferreira, Anna L.B.
Taba Jr, Mario
Gerlach, Raquel F.
Marcaccini, Andrea M.
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  surname: Marcaccini
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  organization: Department of Buco-Maxilo-Facial Surgery and Periodontology
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  givenname: Cesar A.
  surname: Meschiari
  fullname: Meschiari, Cesar A.
  organization: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
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  givenname: Leonardo R.
  surname: Zuardi
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  organization: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
– sequence: 4
  givenname: Tiago Sampaio
  surname: De Sousa
  fullname: De Sousa, Tiago Sampaio
  organization: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
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  surname: Taba Jr
  fullname: Taba Jr, Mario
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  givenname: Juliana M.
  surname: Teofilo
  fullname: Teofilo, Juliana M.
  organization: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
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  givenname: Anna L.B.
  surname: Jacob-Ferreira
  fullname: Jacob-Ferreira, Anna L.B.
  organization: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, SP, Brazil
– sequence: 8
  givenname: Jose E.
  surname: Tanus-Santos
  fullname: Tanus-Santos, Jose E.
  organization: Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
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  givenname: Arthur B.
  surname: Novaes Jr
  fullname: Novaes Jr, Arthur B.
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  givenname: Raquel F.
  surname: Gerlach
  fullname: Gerlach, Raquel F.
  organization: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
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IsPeerReviewed true
IsScholarly true
Issue 2
Keywords TIMP-2
TIMP-1
Stomatology
Crevicular fluid
chronic periodontitis
Dentistry
GCF
MPO
non-surgical therapy
Chronic
Periodontal disease
Treatment
Periodontitis
Surgery
gingival crevicular fluid
MMP-8
MMP-9
Gingival fluid
Language English
License http://onlinelibrary.wiley.com/termsAndConditions#vor
CC BY 4.0
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Conflict of interest and sources of funding statement
The authors declare that they have no conflict of interests.
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PublicationDate February 2010
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  year: 2010
  text: February 2010
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PublicationPlace Oxford, UK
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PublicationTitle Journal of clinical periodontology
PublicationTitleAlternate J Clin Periodontol
PublicationYear 2010
Publisher Blackwell Publishing Ltd
Blackwell
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– name: Blackwell
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Tuter, G., Kurtis, B., Serdar, M., Yucel, A., Ayhan, E., Karaduman, B. & Ozcan, G. (2005) Effects of phase I periodontal treatment on gingival crevicular fluid levels of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1. Journal of Clinical Periodontology 32, 1011-1015.
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Gurkan, A., Cinarcik, S. & Huseyinov, A. (2005) Adjunctive subantimicrobial dose doxycycline: effect on clinical parameters and gingival crevicular fluid transforming growth factor-beta levels in severe, generalized chronic periodontitis. Journal of Clinical Periodontology 32, 244-253.
Choi, D. H., Moon, I. S., Choi, B. K., Paik, J. W., Kim, Y. S., Choi, S. H. & Kim, C. K. (2004) Effects of sub-antimicrobial dose doxycycline therapy on crevicular fluid MMP-8, and gingival tissue MMP-9, TIMP-1 and IL-6 levels in chronic periodontitis. Journal of Periodontal Research 39, 20-26.
Mainardi, C. L., Hibbs, M. S., Hasty, K. A. & Seyer, J. M. (1984) Purification of a type V collagen degrading metalloproteinase from rabbit alveolar macrophages. College Related Research 4, 479-492.
Sengelov, H., Boulay, F., Kjeldsen, L. & Borregaard, N. (1994) Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Biochemical Journal 299 (Part 2), 473-479.
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Olson, M. W., Bernardo, M. M., Pietila, M., Gervasi, D. C., Toth, M., Kotra, L. P., Massova, I., Mobashery, S. & Fridman, R. (2000) Characterization of the monomeric and dimeric forms of latent and active matrix metalloproteinase-9. Differential rates for activation by stromelysin 1. Journal of Biological Chemistry 275, 2661-2668.
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Golub, L. M., Lee, H. M., Stoner, J. A., Sorsa, T., Reinhardt, R. A., Wolff, M. S., Ryan, M. E., Nummikoski, P. V. & Payne, J. B. (2008) Subantimicrobial-dose doxycycline modulates gingival crevicular fluid biomarkers of periodontitis in postmenopausal osteopenic women. Journal of Periodontology 79, 1409-1418.
Kiili, M., Cox, S. W., Chen, H. Y., Wahlgren, J., Maisi, P., Eley, B. M., Salo, T. & Sorsa, T. (2002) Collagenase-2 (MMP-8) and collagenase-3 (MMP-13) in adult periodontitis: molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue. Journal of Clinical Periodontology 29, 224-232.
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– reference: Kowolik, M. J. & Grant, M. (1983) Myeloperoxidase activity in human gingival crevicular neutrophils. Archives of Oral Biology 28, 293-295.
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Snippet Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob‐Ferreira ALB, Tanus‐Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid...
This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in...
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StartPage 180
SubjectTerms Adult
Biological and medical sciences
Case-Control Studies
chronic periodontitis
Chronic Periodontitis - enzymology
Chronic Periodontitis - immunology
Chronic Periodontitis - therapy
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
GCF
Gingiva - enzymology
gingival crevicular fluid
Gingival Crevicular Fluid - enzymology
Gingival Crevicular Fluid - immunology
Humans
Male
Matrix Metalloproteinase 8 - metabolism
Matrix Metalloproteinase 9 - metabolism
Medical sciences
Middle Aged
MMP-8
MMP-9
MPO
Non tumoral diseases
non-surgical therapy
Otorhinolaryngology. Stomatology
Peroxidase - metabolism
Reference Values
Statistics, Nonparametric
TIMP-1
TIMP-2
Tissue Inhibitor of Metalloproteinase-1 - metabolism
Tissue Inhibitor of Metalloproteinase-2 - metabolism
Treatment Outcome
Title Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy
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https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1600-051X.2009.01512.x
https://www.ncbi.nlm.nih.gov/pubmed/19995403
https://www.proquest.com/docview/734011503
Volume 37
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