Simple diagnostic assay for hairy cell leukaemia by immunocytochemical detection of annexin A1 (ANXA1)

• Published in: The Lancet (British edition) Vol. 363; no. 9424; pp. 1869 - 1871
• Main Authors: Falini, Brunangelo, Tiacci, Enrico, Liso, Arcangelo, Basso, Katia, Sabattini, Elena, Pacini, Roberta, Foa, Robin, Pulsoni, Alessandro, Favera, Riccardo Dalla, Pileri, Stefano
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 05.06.2004; Lancet; Elsevier Limited
• Subjects: Annexin A1 - analysis; Annexin A1 - genetics; Assaying; Biological and medical sciences; Biomarkers, Tumor - analysis; Biopsy; Bone marrow; Diagnosis, Differential; Diagnostic systems; Gene Expression; Gene Expression Profiling; General aspects; Genes; Hematologic and hematopoietic diseases; Humans; Immunohistochemistry; Leukemia; Leukemia, Hairy Cell - diagnosis; Leukemia, Hairy Cell - genetics; Leukemia, Hairy Cell - metabolism; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphocytes; Lymphocytes B; Lymphoma; Medical diagnosis; Medical procedures; Medical sciences; Monoclonal antibodies; Morphology; Proteins; Sensitivity and Specificity; Spleen; Tumors; United States > US; Assay; Human; Medicine; Chronic; Hairy cell leukemia; Biochemical analysis; Lymphoproliferative syndrome; Malignant hemopathy; Diagnosis; Detection
• ISSN: 0140-6736; 1474-547X; 1474-547X
• DOI: 10.1016/S0140-6736(04)16356-3

A marker capable of distinguishing with certainty between hairy cell leukaemia and other B-cell malignant disease would be of great diagnostic value. Through gene expression profiling, annexin A1 (ANXA1) has been identified as a gene that is upregulated in hairy cell leukaemia. We did immunostaining of 500 B-cell tumours with a specific anti-ANXA1 monoclonal antibody and showed that ANXA1 protein expression is specific to hairy cell leukaemia. Immunocytochemical detection of ANXA1 represents a simple, inexpensive, highly sensitive and specific (100%) assay for diagnosis of hairy cell leukaemia. This assay will be especially useful in distinguishing hairy cell leukaemia from splenic lymphoma with villous lymphocytes and variant hairy cell leukaemia, both of which usually respond poorly to treatments that are effective in hairy cell leukaemia.