PhaBOX: a web server for identifying and characterizing phage contigs in metagenomic data

• Published in: Bioinformatics advances Vol. 3; no. 1; p. vbad101
• Main Authors: Shang, Jiayu, Peng, Cheng, Liao, Herui, Tang, Xubo, Sun, Yanni
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 2023
• Subjects: Application Note; Informatics; Internet; Metagenomics; Microbiomes; Phages; Predictions
• ISSN: 2635-0041; 2635-0041
• DOI: 10.1093/bioadv/vbad101

Abstract Motivation There is accumulating evidence showing the important roles of bacteriophages (phages) in regulating the structure and functions of the microbiome. However, lacking an easy-to-use and integrated phage analysis software hampers microbiome-related research from incorporating phages in the analysis. Results In this work, we developed a web server, PhaBOX, which can comprehensively identify and analyze phage contigs in metagenomic data. It supports integrated phage analysis, including phage contig identification from the metagenomic assembly, lifestyle prediction, taxonomic classification, and host prediction. Instead of treating the algorithms as a black box, PhaBOX also supports visualization of the essential features for making predictions. The web server is designed with a user-friendly graphical interface that enables both informatics-trained and nonspecialist users to analyze phages in microbiome data with ease. Availability and implementation The web server of PhaBOX is available via: https://phage.ee.cityu.edu.hk. The source code of PhaBOX is available at: https://github.com/KennthShang/PhaBOX.