First Look at the Venom of Naja ashei
Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an a...
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| Published in | Molecules (Basel, Switzerland) Vol. 23; no. 3; p. 609 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
MDPI AG
08.03.2018
MDPI |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1420-3049 1420-3049 |
| DOI | 10.3390/molecules23030609 |
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| Abstract | Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2). Additionally the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras—cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. |
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| AbstractList | Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2). Additionally the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras—cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. is an African spitting cobra species closely related to and . It is known that the venom of , like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA₂s (Phospholipases A₂). Additionally the presence of cysteine-rich venom proteins, 5'-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of includes proteins not described previously in other African spitting cobras-cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA₂s (Phospholipases A₂). Additionally the presence of cysteine-rich venom proteins, 5'-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras-cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species.Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA₂s (Phospholipases A₂). Additionally the presence of cysteine-rich venom proteins, 5'-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras-cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. |
| Author | Hus, Konrad Łyskowski, Andrzej Bocian, Aleksandra Petrillová, Monika Legáth, Jaroslav Petrilla, Vladimír Buczkowicz, Justyna |
| AuthorAffiliation | 2 Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia; petrillav@gmail.com 4 Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia; monika.petrillova@uvlf.sk 1 Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, Poland; knr.hus@gmail.com (K.K.H.); czaporj@prz.edu.pl (J.B.); alyskowski@prz.edu.pl (A.Ł.); Jaroslav.Legath@uvlf.sk (J.L.) 3 Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovakia 5 Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia |
| AuthorAffiliation_xml | – name: 4 Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia; monika.petrillova@uvlf.sk – name: 3 Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovakia – name: 5 Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia – name: 2 Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia; petrillav@gmail.com – name: 1 Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, Poland; knr.hus@gmail.com (K.K.H.); czaporj@prz.edu.pl (J.B.); alyskowski@prz.edu.pl (A.Ł.); Jaroslav.Legath@uvlf.sk (J.L.) |
| Author_xml | – sequence: 1 givenname: Konrad orcidid: 0000-0003-2723-3691 surname: Hus fullname: Hus, Konrad – sequence: 2 givenname: Justyna surname: Buczkowicz fullname: Buczkowicz, Justyna – sequence: 3 givenname: Vladimír surname: Petrilla fullname: Petrilla, Vladimír – sequence: 4 givenname: Monika surname: Petrillová fullname: Petrillová, Monika – sequence: 5 givenname: Andrzej orcidid: 0000-0001-8625-4164 surname: Łyskowski fullname: Łyskowski, Andrzej – sequence: 6 givenname: Jaroslav surname: Legáth fullname: Legáth, Jaroslav – sequence: 7 givenname: Aleksandra orcidid: 0000-0003-1907-9399 surname: Bocian fullname: Bocian, Aleksandra |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29518026$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | 2-D electrophoresis Animals Communication Desorption Elapid Venoms - chemistry Elapid Venoms - metabolism Ions Naja - metabolism Naja ashei proteomics Proteomics - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization venom composition |
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| Title | First Look at the Venom of Naja ashei |
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