Characterization of flow and mixing regimes within the ileum of the brushtail possum using residence time distribution analysis with simultaneous spatio-temporal mapping

• Published in: The Journal of physiology Vol. 582; no. 3; pp. 1239 - 1248
• Main Authors: Janssen, P. W. M., Lentle, R. G., Asvarujanon, P., Chambers, P., Stafford, K. J., Hemar, Y.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 01.08.2007; Blackwell Publishing Ltd; Blackwell Science Inc
• Subjects: Alimentary; Animals; Calibration; Galactans; Gastrointestinal Motility - physiology; Ileum - cytology; Ileum - physiology; In Vitro Techniques; Kinetics; Mannans; Muscle, Smooth - cytology; Muscle, Smooth - physiology; Opossums; Oscillometry; Plant Gums; Solutions; Time Factors
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.2007.134403

We studied the flow and mixing regimes in isolated segments of the terminal ileum of brushtail possums during spontaneous circumferential and longitudinal contractions under conditions that allowed backflow and compared them with those of inactive segments. Residence time distributions (RTDs) were determined by perfusion with two probes of different rheological properties to which an inert dye marker was added. Ileal segment volume and oscillatory flow during the period of RTD determination were derived from spatiotemporal maps. High viscosity guar gum solution generated RTDs characteristic of laminar flow in inactive ileal segments which confirmed that no slip was occurring at the mucosal layer. In active segments, motility and consequent oscillatory flow imparted significant additional axial dispersion to the flow patterns of both probes. Mixing occurred episodically during periods when intestinal volume was reduced and onflow was augmented by peristalsis, which may prevent the establishment of steady state conditions. Marker concentration rose more steeply when active ileal segments were being perfused with a probe of similar viscosity to normal digesta than with low viscosity Earle's/Hepes solution, each being subject to similar levels of oscillatory flow. This indicated that a coarser mixing regime prevailed and that absorption of nutrients from viscous digesta would rely to a greater degree on molecular diffusion.