Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes
The aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue - BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicula...
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| Published in | Veterinární medicína Vol. 56; no. 4; pp. 156 - 167 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Prague
Czech Academy of Agricultural Sciences (CAAS)
01.01.2011
Czech Academy of Agricultural Sciences |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0375-8427 1805-9392 1805-9392 |
| DOI | 10.17221/1573-vetmed |
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| Abstract | The aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue - BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB+ and BCB-). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P less than 0.05). With regard to individual fatty acids, only C16:0, C18:2n-6 and C22:6 differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB+/BCB-), whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB+ oocytes this may be considered as a marker of oocyte quality in the pig. |
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| AbstractList | Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular cells which accompany the growing oocyte. Although nutrients present in the diet reach oocytes via the follicular fluid, it is not evident whether oocyte/embryo quality can be predicted based on the follicular fluid composition. Since this phenomenon has not yet been investigated in the pig, the aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue – BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB+ and BCB–). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P < 0.05). With regard to individual fatty acids, only C16:0 (palmitic acid), C18:2n-6 (linoleic acid) and C22:6 (docosahexaenoic acid) differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB +/BCB –) whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB+ oocytes this may be considered as a marker of oocyte quality in the pig. The aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue - BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB+ and BCB-). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P less than 0.05). With regard to individual fatty acids, only C16:0, C18:2n-6 and C22:6 differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB+/BCB-), whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB+ oocytes this may be considered as a marker of oocyte quality in the pig. |
| Author | Warzych, E.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology Pers-Kamczyc, E.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology Pawlak, P.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology Cieslak, A.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology Renska, N.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology Lechniak, D.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology |
| Author_xml | – sequence: 1 fullname: Warzych, E.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology – sequence: 2 fullname: Cieslak, A.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology – sequence: 3 fullname: Pawlak, P.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology – sequence: 4 fullname: Renska, N.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology – sequence: 5 fullname: Pers-Kamczyc, E.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology – sequence: 6 fullname: Lechniak, D.,University of Life Sciences, Lublin (Poland). Faculty of Animal Breeding and Biology |
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| Snippet | The aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes... Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the... |
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| SubjectTerms | Abundance ACIDE GRAS INSATURE ACIDE GRAS POLYINSATURE ACIDE GRAS SATURE ACIDOS GRASOS INSATURADOS ACIDOS GRASOS POLIINSATURADOS ACIDOS GRASOS SATURADOS ALIMENT POUR ANIMAUX ALIMENTACION DE LOS ANIMALES ALIMENTATION DES ANIMAUX ANIMAL FEEDING ANIMAL NUTRITION atp5a1 barley bcb test Biomarkers CALIDAD CELLS CELLULE CELULAS CERDAS CERDO CHEMICAL COMPOSITION COMPOSICION QUIMICA Composition COMPOSITION CHIMIQUE DIET DIETA Docosahexaenoic acid eef1a1 Embryos ENSAYO experimental diets Fatty acid composition Fatty acids FEEDS Follicular fluid Gametocytes Gas chromatography GENE gene expression GENES GENETIC MARKERS gilts http://www.fao.org/aos/agrovoc#c_1418 http://www.fao.org/aos/agrovoc#c_1794 http://www.fao.org/aos/agrovoc#c_2261 http://www.fao.org/aos/agrovoc#c_24030 http://www.fao.org/aos/agrovoc#c_27925 http://www.fao.org/aos/agrovoc#c_2843 http://www.fao.org/aos/agrovoc#c_29238 http://www.fao.org/aos/agrovoc#c_29245 http://www.fao.org/aos/agrovoc#c_29254 http://www.fao.org/aos/agrovoc#c_3214 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_429 http://www.fao.org/aos/agrovoc#c_5454 http://www.fao.org/aos/agrovoc#c_5456 http://www.fao.org/aos/agrovoc#c_6400 http://www.fao.org/aos/agrovoc#c_6507 http://www.fao.org/aos/agrovoc#c_7271 http://www.fao.org/aos/agrovoc#c_7555 http://www.fao.org/aos/agrovoc#c_7683 Human nutrition Linoleic acid MARCADORES GENETICOS MARQUEUR GENETIQUE maternal nutrition Metabolites Metabolomics NUTRICION ANIMAL Nutrients Nutrition NUTRITION ANIMALE omega-3 fatty acids Oocytes OVA OVAIRE OVARIES OVARIOS OVULE OVULO Palmitic acid PCR PIENSOS pig Poland POLYUNSATURATED FATTY ACIDS PORCIN QUALITE QUALITY quantitative polymerase chain reaction REGIME ALIMENTAIRE REPRODUCCION REPRODUCTION Rye SATURATED FATTY ACIDS slaughter SOWS SWINE TESTAGE TESTING Transcription TRUIE UNSATURATED FATTY ACIDS |
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| Title | Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes |
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