Overcoming Near-Cognate Suppression in a Release Factor 1-Deficient Host with an Improved Nitro-Tyrosine tRNA Synthetase - Beyer, Jenna N.; Hosseinzadeh, Parisa; Gottfried-Lee, Ilana; Van Fossen, Elise M.; Zhu, Phillip; Bednar, Riley M.; Karplus, P. Andrew; Mehl, Ryan A.; Cooley, Richard B. (Journal of molecular biology, 2020)
Keywords: active sites; amber; Amino Acyl-tRNA Synthetases - chemistry; Amino Acyl-tRNA Synthetases - genetics; Amino Acyl-tRNA Synthetases - metabolism; Catalytic Domain; Codon, Terminator; Computer Simulation; Crystallography, X-Ray; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins; genetic code; genetic code expansion; Genetic Engineering; Models, Molecular; Mutation; near-cognate suppression; nitro-tyrosine; Peptide Termination Factors - deficiency; Protein Binding; Protein Conformation; proteins; Release Factor 1; stop codon; tRNA synthetase; Tyrosine - analogs & derivatives; Tyrosine - metabolism; tyrosine-tRNA ligase; TCO-K; ncAA; GCE; genetic code expansion; Tet2.0; tRNA synthetase; Release Factor 1; aaRS; nitroTyr; AzF; Bpa; nitro-tyrosine; near-cognate suppression; sfGFP; RF1