Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination

Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells tha...

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Published inJournal of immunological methods Vol. 502; p. 113230
Main Authors Grimaldi, Vincenzo, Benincasa, Giuditta, Moccia, Giusi, Sansone, Annunziata, Signoriello, Giuseppe, Napoli, Claudio
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.2022
Subjects
Adult
Aged
Case-Control Studies
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
COVID-19 - blood
COVID-19 - immunology
COVID-19 - prevention & control
COVID-19 - virology
COVID-19 infection
COVID-19 mRNA BNT162b2 (Pfizer-BioNTech) vaccine
COVID-19 Vaccines - administration & dosage
COVID-19 Vaccines - immunology
Cross-Sectional Studies
Female
Flow cytometry characterization
Healthy Volunteers
Humans
Immunity, Innate
Immunogenicity, Vaccine
Immunophenotyping
innate immunity
Male
memory
Memory T Cells - immunology
Middle Aged
SARS-CoV-2 - immunology
Severe acute respiratory syndrome coronavirus 2
T cell immune response
Technical Note
Vaccination
vaccines
virus replication
Flow cytometry characterization
COVID-19 infection
COVID-19 mRNA BNT162b2 (Pfizer-BioNTech) vaccine
T cell immune response
Online AccessGet full text
ISSN0022-1759
1872-7905
1872-7905
DOI10.1016/j.jim.2022.113230

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Abstract Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8+ T cells (p < 0,05) decrease and an augmented CD4+/CD8+ ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8+ T cells, and a reduction of CD4+/CD8+ ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.
AbstractList Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8+ T cells (p < 0,05) decrease and an augmented CD4+/CD8+ ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8+ T cells, and a reduction of CD4+/CD8+ ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8+ T cells (p < 0,05) decrease and an augmented CD4+/CD8+ ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8+ T cells, and a reduction of CD4+/CD8+ ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.
Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8+ T cells (p < 0,05) decrease and an augmented CD4+/CD8+ ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8+ T cells, and a reduction of CD4+/CD8+ ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.
Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8⁺ T cells (p < 0,05) decrease and an augmented CD4⁺/CD8⁺ ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8⁺ T cells, and a reduction of CD4⁺/CD8⁺ ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.
Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on viral replication. Moreover, innate immune response mobilizes cells of the cellular-mediated immune system, which develop into effector cells that promote viral clearance. Here, we observed circulating leukocyte T cell response in healthy subjects, COVID-19 infected, and in healthy vaccinated subjects. We found a significant CD8 T cells (p < 0,05) decrease and an augmented CD4 /CD8 ratio (p < 0,05) in COVID-19 infected group compared with vaccinated subjects. In addition, healthy vaccinated subjects have a significant increased expression of CD8 T cells, and a reduction of CD4 /CD8 ratio with respect to subjects previously COVID-19 infected. Central Memory and Terminal Effector Memory cells (TEMRA) increased after vaccine but not among groups.
ArticleNumber 113230
Author Sansone, Annunziata
Napoli, Claudio
Moccia, Giusi
Signoriello, Giuseppe
Grimaldi, Vincenzo
Benincasa, Giuditta
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  surname: Napoli
  fullname: Napoli, Claudio
  organization: U.O.C. Division of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology. Clinical Department of Internal Medicine and Specialistic Units, University of Campania "L. Vanvitelli", 80138 Naples, Italy
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Keywords Flow cytometry characterization
COVID-19 infection
COVID-19 mRNA BNT162b2 (Pfizer-BioNTech) vaccine
T cell immune response
Language English
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Snippet Innate immune mechanisms are central players in response to the binding of pathogens to pattern-recognition receptors providing a crucial initial block on...
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SubjectTerms Adult
Aged
Case-Control Studies
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
COVID-19 - blood
COVID-19 - immunology
COVID-19 - prevention & control
COVID-19 - virology
COVID-19 infection
COVID-19 mRNA BNT162b2 (Pfizer-BioNTech) vaccine
COVID-19 Vaccines - administration & dosage
COVID-19 Vaccines - immunology
Cross-Sectional Studies
Female
Flow cytometry characterization
Healthy Volunteers
Humans
Immunity, Innate
Immunogenicity, Vaccine
Immunophenotyping
innate immunity
Male
memory
Memory T Cells - immunology
Middle Aged
SARS-CoV-2 - immunology
Severe acute respiratory syndrome coronavirus 2
T cell immune response
Technical Note
Vaccination
vaccines
virus replication
Title Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination
URI https://dx.doi.org/10.1016/j.jim.2022.113230
https://www.ncbi.nlm.nih.gov/pubmed/35114198
https://www.proquest.com/docview/2626008469
https://www.proquest.com/docview/2648864335
https://pubmed.ncbi.nlm.nih.gov/PMC8802555
Volume 502
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