Engineering Archeal Surrogate Systems for the Development of Protein–Protein Interaction Inhibitors against Human RAD51 - Moschetti, Tommaso; Sharpe, Timothy; Fischer, Gerhard; Marsh, May E.; Ng, Hong Kin; Morgan, Matthew; Scott, Duncan E.; Blundell, Tom L.; R. Venkitaraman, Ashok; Skidmore, John; Abell, Chris; Hyvönen, Marko (Journal of molecular biology, 2016)
Keywords: Archaeal Proteins - genetics; Archaeal Proteins - metabolism; binding sites; BRCA2 Protein - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Drug Discovery - methods; drugs; Enzyme Inhibitors - isolation & purification; fragment-based drug discovery; humanisation; Humans; Mutant Proteins - genetics; Mutant Proteins - metabolism; neoplasms; process design; Protein Binding - drug effects; protein engineering; Protein Engineering - methods; protein-protein interactions; Pyrococcus - enzymology; Pyrococcus furiosus; Rad51 Recombinase - genetics; Rad51 Recombinase - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; recombinase; sequence analysis; stoichiometry; surrogate system; tumor suppressor proteins; humanisation; TEV; DMSO; PPI; GST; HR; FP; DLS; surrogate system; protein engineering; fragment-based drug discovery; PEG; DSF; NF; ITC; HumRadA1; recombinase; BRC4