Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

• Published in: Acta biomaterialia Vol. 76; pp. 126 - 134
• Main Authors: Lee, Kwang Il, Olmer, Merissa, Baek, Jihye, D'Lima, Darryl D., Lotz, Martin K.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2018; Elsevier BV
• Subjects: Animals; Biocompatibility; Biomechanics; Biomedical materials; Blood platelets; Cattle; Cell adhesion & migration; Cell culture; Cell migration; Cells (biology); Chemotactic response; Chondrocytes; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacokinetics; Coated Materials, Biocompatible - pharmacology; Coating; Coatings; Collagen; Controlled release; Decellularization; Defects; Explants; Extracellular matrix; Growth factors; Healing; Heparin; Humans; Immobilization; Injuries; Insertion; Integration; Joint and ligament injuries; Knee; Knee Injuries - metabolism; Knee Injuries - therapy; Mechanical properties; Meniscus; Organic chemistry; Osteoarthritis; PDGF; Platelet-derived growth factor; Platelet-Derived Growth Factor - chemistry; Platelet-Derived Growth Factor - pharmacokinetics; Platelet-Derived Growth Factor - pharmacology; Platelet-derived growth factor BB; Progenitor cells; Risk analysis; Risk factors; Safranin-O; Scaffolds; Staining; Stem cells; Sustained release; Tensile properties; Tissue Engineering; Tissue Scaffolds - chemistry; Wound healing; Meniscus; Heparin; PDGF; Cell migration; Decellularization
• ISSN: 1742-7061; 1878-7568; 1878-7568
• DOI: 10.1016/j.actbio.2018.06.021

[Display omitted] The aim of this study was to examine the potential of platelet-derived growth factor (PDGF)-coated decellularized meniscus scaffold in mediating integrative healing of meniscus tears by inducing endogenous cell migration. Fresh bovine meniscus was chemically decellularized and covalently conjugated with heparin and PDGF-BB. In vitro PDGF release kinetics was measured. The scaffold was transplanted into experimental tears in avascular bovine meniscus explants and cultured for 2 and 4 weeks. The number migrating and proliferating cells at the borderline between the scaffold and injured explant and PDGF receptor-β (PDGFRβ) expressing cells were counted. The alignment of the newly produced ECM and collagen was analyzed by Safranin-O, picrosirius red staining, and differential interference contrast (DIC). Tensile testing of the explants was performed after culture for 2 and 4 weeks. Heparin conjugated scaffold showed immobilization of high levels of PDGF-BB, with sustained release over 2 weeks. Insertion of the PDGF-BB treated scaffold in defects in avascular meniscus led to increased PDGFRβ expression, cell migration and proliferation into the defect zone. Safranin-O, picrosirius red staining and DIC showed tissue integration between the scaffold and injured explants. Tensile properties of injured explants treated with PDGF-BB coated scaffold were significantly higher than in the scaffold without PDGF. In conclusion, PDGF-BB-coated scaffold increased PDGFRβ expression and promoted migration of endogenous meniscus cells to the defect area. New matrix was formed that bridged the space between the native meniscus and the scaffold and this was associated with improved biomechanical properties. The PDGF-BB-coated scaffold will be promising for clinical translation to healing of meniscus tears. Meniscus tears are the most common injury of the knee joint. The most prevalent forms that occur in the inner third typically do not spontaneously heal and represent a major risk factor for the development of knee osteoarthritis. The goal of this project was to develop an approach that is readily applicable for clinical use. We selected a natural and readily available decellularized meniscus scaffold and conjugated it with PDGF, which we had previously found to have strong chemotactic activity for chondrocytes and progenitor cells. The present results show that insertion of the PDGF-conjugated scaffold in defects in avascular meniscus led to endogenous cell migration and proliferation into the defect zone with tissue integration between the scaffold and injured explants and improved tensile properties. This PDGF-conjugated scaffold will be promising for a translational approach to healing of meniscus tears.