Development and validation of a hydrophilic interaction chromatography–tandem mass spectrometry method with on-line polar extraction for the analysis of urinary nucleosides. Potential application in clinical diagnosis - Rodríguez-Gonzalo, Encarnación; García-Gómez, Diego; Carabias-Martínez, Rita (Journal of Chromatography A, 2011)
Keywords: Bioanalytical method validation; Biological and medical sciences; Calibration; Cancer diagnosis; Chromatography, Liquid - methods; Cluster Analysis; composite polymers; detection limit; Drug Stability; Host-tumor relations. Immunology. Biological markers; Humans; hydrophilic interaction chromatography; hydrophilicity; Hydrophobic and Hydrophilic Interactions; laws and regulations; Limit of Detection; Medical sciences; Neoplasms - urine; nucleosides; Nucleosides - urine; On-line sample extraction; patients; Pattern Recognition, Automated; Principal Component Analysis; Reproducibility of Results; Restricted-access material (RAM); solid phase extraction; Solid Phase Extraction - methods; tandem mass spectrometry; Tandem Mass Spectrometry - methods; Tumors; urinalysis; Urine; volunteers; Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC); zwitterions; Urine; Restricted-access material (RAM); Bioanalytical method validation; Cancer diagnosis; Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC); On-line sample extraction; Cluster analysis; Solid phase extraction; Biological fluid; Tandem mass spectrometry; Purine derivatives; Chemical analysis; On line; Zwitterionic hydrophilic interaction; HPLC chromatography; Biological marker; Zwitterion; Chemical enrichment; Sample preparation; Nucleoside; Diagnosis; Quantitative analysis; Trace analysis; Human; Validation; Healthy subject; Restricted access medium column; Hierarchical classification; Coupled method; Hydrophilic Interaction Liquid chromatography; Elimination; Patient; Malignant tumor; chromatography (ZIC-HILIC); Pyrimidine derivatives; Chemometrics; Bonded stationary phase; Cancer; Principal component analysis