Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development
Abstract Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive o...
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| Published in | Medical principles and practice Vol. 33; no. 2; pp. 112 - 121 |
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| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Basel, Switzerland
S. Karger AG
01.04.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1011-7571 1423-0151 1423-0151 |
| DOI | 10.1159/000536358 |
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| Abstract | Abstract
Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.
Highlights of the StudyWe compared the results of IVF between two experimental setups: light protection with aluminum foil and red filters, and no light protection as a control.Conventional embryo culture conditions were improved by using special light filters for light protection.Reducing the embryotoxic wavelengths of light in IVF centers improved the development rate of embryos and also embryo quality, while maintaining embryo safety. |
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| AbstractList | Abstract
Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.
Highlights of the StudyWe compared the results of IVF between two experimental setups: light protection with aluminum foil and red filters, and no light protection as a control.Conventional embryo culture conditions were improved by using special light filters for light protection.Reducing the embryotoxic wavelengths of light in IVF centers improved the development rate of embryos and also embryo quality, while maintaining embryo safety. Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).OBJECTIVEEarly embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control.MATERIALS AND METHODSWe tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control.Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate.RESULTSBlastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate.The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.CONCLUSIONSThe implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. The success rate of assisted reproductive technology (ART) can vary depending on several factors, including the age and health of the individuals involved, the quality of the oocytes and sperm, the expertise of the medical team, and the specific techniques and technologies used. IVF and intracytoplasmic sperm injection (ICSI), however, may result in harmful exposure of cells to light during several steps of these procedures, such as oocyte retrieval, sperm preparation, placement of cultures in and out of incubators as well as during microscopy, fertilization checks, morphological assessment, and embryo transfer. [...]it would be ethically untenable to subject some of our patients to a potentially lower quality of care. [...]our study can be considered as a “same before” group with an expanded “after group” retrospective study, where the control group represents our former patients with similar clinical characteristics (Table 1) but without LP. Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. |
| Author | Herczeg, Róbert Erostyák, János Bódis, József Kovács, Gábor L. Bognár, Zoltán Nagy, Bernadett Gödöny, Krisztina Várnagy, Ákos Kovács, Kálmán Mauchart, Péter Gyenesei, Attila Szekeres-Barthó, Júlia |
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| Keywords | In vitro fertilization Light protection Live birth rate Intracytoplasmic sperm injection Abortion Blastocyst |
| Language | English |
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| Snippet | Abstract
Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to... Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental... Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences.... The success rate of assisted reproductive technology (ART) can vary depending on several factors, including the age and health of the individuals involved, the... |
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| SubjectTerms | Adult Aluminum Blastocyst - physiology Blastocyst - radiation effects Cell division DNA damage DNA repair Embryo Culture Techniques - methods Embryonic Development - physiology Embryonic Development - radiation effects Embryos Female Females Fertilization in Vitro - adverse effects Fertilization in Vitro - methods Humans In vitro fertilization Infertility Laboratories Light - adverse effects Male Original Paper Oxidation Oxidative Stress - physiology Oxidative Stress - radiation effects Physiology Reproductive technologies Retrospective Studies Sperm Sperm Injections, Intracytoplasmic - adverse effects Sperm Injections, Intracytoplasmic - methods Success |
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| Title | Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development |
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