Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration

• Published in: Diabetes, metabolic syndrome and obesity Vol. 13; pp. 3009 - 3034
• Main Authors: Guan, Chun-Yi, Tian, Shi, Cao, Jing-Li, Wang, Xue-Qin, Ma, Xu, Xia, Hong-Fei
• Format: Journal Article
• Language: English
• Published: New Zealand Taylor & Francis Ltd 01.01.2020; Dove; Dove Medical Press
• Subjects: Acids; Cell culture; Family planning; Gene expression; Gestational diabetes; gestational diabetes mellitus; Glucose; Hybridization; Insulin resistance; Laboratory animals; Maternal & child health; Metabolism; MicroRNAs; mirna-21; Original Research; ppar-α; Pregnancy; Womens health; United States > US; China; Germany; gestational diabetes mellitus; PPAR-α; insulin resistance; miRNA-21
• ISSN: 1178-7007; 1178-7007
• DOI: 10.2147/DMSO.S253920

This study aimed to investigate the role of expression in the reduction of placental function in GDM patients. qRT-PCR was used to detect the differential expression of in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of on cell function. is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of but increased expression of  was observed in patients with GDM and GDM rats. The expression of the placental , which inhibits cell growth and infiltration by up-regulating , is downregulated in pregnant GDM patients, which in turn may affect the placental function.