Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration
This study aimed to investigate the role of expression in the reduction of placental function in GDM patients. qRT-PCR was used to detect the differential expression of in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene of miR-21 b...
Saved in:
| Published in | Diabetes, metabolic syndrome and obesity Vol. 13; pp. 3009 - 3034 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
New Zealand
Taylor & Francis Ltd
01.01.2020
Dove Dove Medical Press |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1178-7007 1178-7007 |
| DOI | 10.2147/DMSO.S253920 |
Cover
| Abstract | This study aimed to investigate the role of
expression in the reduction of placental function in GDM patients.
qRT-PCR was used to detect the differential expression of
in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene
of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of
on cell function.
is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance,
knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR
expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of
but increased expression of
was observed in patients with GDM and GDM rats.
The expression of the placental
, which inhibits cell growth and infiltration by up-regulating
, is downregulated in pregnant GDM patients, which in turn may affect the placental function. |
|---|---|
| AbstractList | This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.PURPOSEThis study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.MATERIALS AND METHODSqRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.RESULTSmiR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.CONCLUSIONThe expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function. This study aimed to investigate the role of expression in the reduction of placental function in GDM patients. qRT-PCR was used to detect the differential expression of in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of on cell function. is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of but increased expression of was observed in patients with GDM and GDM rats. The expression of the placental , which inhibits cell growth and infiltration by up-regulating , is downregulated in pregnant GDM patients, which in turn may affect the placental function. Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People's Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People's Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People's Republic of China*These authors contributed equally to this workCorrespondence: Xu Ma; Hong-Fei XiaReproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People's Republic of ChinaTel +86-10-62178932Fax +86-10-62179059Email xuma_genetics@163.com; hongfeixia@126.comPurpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-αwas observed in patients with GDM and GDM rats.Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-α Purpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients. Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function. Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats. Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function. |
| Author | Guan, Chun-Yi Tian, Shi Cao, Jing-Li Wang, Xue-Qin Xia, Hong-Fei Ma, Xu |
| Author_xml | – sequence: 1 givenname: Chun-Yi surname: Guan fullname: Guan, Chun-Yi – sequence: 2 givenname: Shi surname: Tian fullname: Tian, Shi – sequence: 3 givenname: Jing-Li surname: Cao fullname: Cao, Jing-Li – sequence: 4 givenname: Xue-Qin surname: Wang fullname: Wang, Xue-Qin – sequence: 5 givenname: Xu surname: Ma fullname: Ma, Xu – sequence: 6 givenname: Hong-Fei orcidid: 0000-0003-3305-9108 surname: Xia fullname: Xia, Hong-Fei |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32943895$$D View this record in MEDLINE/PubMed |
| BookMark | eNptks9u1DAQxiNUREvpjTOyxIUDKf4bOxekahfKSq262sLZchxn65U3bm0HxIV34kV4JpzNFrUVvtia-c2n8XzzsjjofW-K4jWCpxhR_mF-eX11eo0ZqTF8VhwhxEXJIeQHD96HxUmMGzgeDinGL4pDgmtKRM2Oil9z_6MvV2Y9OJVMC7Z2VWIEbA_OTUwqWd8rB-ZWNSaZCC6NczYNESyd0qZPCiz6dtA5s1yerco_v0HyOXRjG5vALMNgGbyznQk7KaD6Nqc769IUeFU875SL5mR_HxffPn_6OvtSXlydL2ZnF6WmXKRSVIQjkz9QISZqUTFYC8WoIrRC-VFjI3THqeCadlATwYjmXYNoa2ArYMXIcbGYdFuvNvI22K0KP6VXVu4CPqylCslqZyQRXVXphrSMCioIazpNjGpJhTXUqK2z1sdJ63ZotqYdxxCUeyT6ONPbG7n23yWnjHE2CrzbCwR_N-Qxy62NOg9L9cYPUWJKszuQI5zRt0_QjR9CtmRHkYpnSmTqzcOO_rVyb3MG8ATo4GMMppPaTubmBq2TCMpxneS4TnK_Trno_ZOie93_4n8BhgrKYQ |
| CitedBy_id | crossref_primary_10_1155_2022_2169259 crossref_primary_10_1038_s41598_022_23816_3 crossref_primary_10_1017_S2040174423000351 crossref_primary_10_1007_s10330_023_0625_5 crossref_primary_10_1016_j_biochi_2021_05_015 crossref_primary_10_3390_epigenomes5020013 crossref_primary_10_1007_s00592_023_02176_y crossref_primary_10_1042_CS20230681 crossref_primary_10_3389_fendo_2021_664287 crossref_primary_10_3390_jpm13071126 crossref_primary_10_31083_j_fbl2902068 crossref_primary_10_3390_ijms24055030 crossref_primary_10_1159_000520340 crossref_primary_10_3390_cells11172672 crossref_primary_10_3390_biology10030218 crossref_primary_10_1186_s12884_025_07405_6 crossref_primary_10_4239_wjd_v15_i3_378 crossref_primary_10_1007_s10815_023_02827_7 crossref_primary_10_1002_ctd2_205 crossref_primary_10_1016_j_repbio_2024_100924 crossref_primary_10_2147_DMSO_S325993 crossref_primary_10_3390_ijms23073514 crossref_primary_10_1016_j_obmed_2023_100487 |
| Cites_doi | 10.1210/en.2009-1122 10.18821/0869-2084-2019-64-12-723-729 10.1016/S1472-6483(10)60135-6 10.26355/eurrev_202001_20062 10.1371/journal.pone.0055272 10.1159/000475912 10.2337/dc07-s202 10.1016/j.phrs.2013.03.011 10.1007/s13205-019-1952-9 10.3389/fimmu.2015.00019 10.1007/s00125-012-2804-x 10.1186/s13000-019-0899-9 10.1073/pnas.012610299 10.1002/jcp.22716 10.1016/j.rbmo.2010.01.002 10.1590/S0104-42302009000400010 10.1055/s-2001-18576 10.1001/jama.1997.03550130052036 10.5603/fhc.a2015.0023 10.1590/S0100-879X2006005000132 10.1002/mgg3.1012 10.1038/nrg2455 10.1038/nm928 10.1128/MCB.00479-08 10.1159/000099149 10.1038/s41598-016-0001-8 10.1161/01.ATV.0000012302.11991.42 10.1186/s12864-015-1969-3 10.1038/sj.onc.1210083 10.1016/j.mce.2006.02.009 10.1016/S0889-8529(18)30055-0 10.2174/13894501113149990182 10.1038/347645a0 |
| ContentType | Journal Article |
| Copyright | 2020 Guan et al. 2020. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2020 Guan et al. 2020 Guan et al. |
| Copyright_xml | – notice: 2020 Guan et al. – notice: 2020. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2020 Guan et al. 2020 Guan et al. |
| DBID | AAYXX CITATION NPM 3V. 7RV 7XB 8C1 8FI 8FJ 8FK 8G5 ABUWG AFKRA AZQEC BENPR CCPQU DWQXO FYUFA GHDGH GNUQQ GUQSH KB0 M2O MBDVC NAPCQ PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA |
| DOI | 10.2147/DMSO.S253920 |
| DatabaseName | CrossRef PubMed ProQuest Central (Corporate) Nursing & Allied Health Database ProQuest Central (purchase pre-March 2016) Public Health Database Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Research Library (Alumni) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student ProQuest Research Library Nursing & Allied Health Database (Alumni Edition) ProQuest Research Library Research Library (Corporate) Nursing & Allied Health Premium ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database Research Library Prep ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing Research Library (Alumni Edition) ProQuest Central China ProQuest Central Health Research Premium Collection ProQuest Central Korea Health & Medical Research Collection ProQuest Research Library ProQuest Central (New) ProQuest Public Health ProQuest Central Basic ProQuest One Academic Eastern Edition ProQuest Nursing & Allied Health Source ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) Nursing & Allied Health Premium ProQuest One Academic UKI Edition ProQuest Nursing & Allied Health Source (Alumni) ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic PubMed Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: BENPR name: ProQuest Central url: http://www.proquest.com/pqcentral?accountid=15518 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| DocumentTitleAlternate | Guan et al |
| EISSN | 1178-7007 |
| EndPage | 3034 |
| ExternalDocumentID | oai_doaj_org_article_38f66cb3d5484835bfc3ead362c0c1d9 PMC7455759 32943895 10_2147_DMSO_S253920 |
| Genre | Journal Article |
| GeographicLocations | United States--US China Germany |
| GeographicLocations_xml | – name: China – name: United States--US – name: Germany |
| GrantInformation_xml | – fundername: ; |
| GroupedDBID | --- 0YH 29F 2WC 53G 5VS 7RV 8C1 8FI 8FJ 8G5 AAYXX ABUWG ACGFO ADBBV ADRAZ AFKRA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BCNDV BENPR BKEYQ BPHCQ BVXVI C1A CCPQU CITATION DIK DWQXO E3Z EBD EBS EJD FYUFA GNUQQ GROUPED_DOAJ GUQSH GX1 HYE IAO IHR IHW IPNFZ ITC KQ8 M2O M48 M~E NAPCQ O5R O5S OK1 P2P PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC RIG RNS RPM TDBHL TR2 UKHRP VDV NPM PJZUB PPXIY 3V. 7XB 8FK MBDVC PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c478t-86371e00061589865098a54a34618a592e8cf7487c4f0c3853c7fb14de0d80653 |
| IEDL.DBID | M48 |
| ISSN | 1178-7007 |
| IngestDate | Wed Aug 27 01:30:39 EDT 2025 Thu Aug 21 18:24:42 EDT 2025 Thu Sep 04 18:40:03 EDT 2025 Fri Jul 25 05:45:41 EDT 2025 Mon Jul 21 05:39:18 EDT 2025 Tue Jul 01 01:45:17 EDT 2025 Thu Apr 24 23:07:36 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | gestational diabetes mellitus PPAR-α insulin resistance miRNA-21 |
| Language | English |
| License | http://creativecommons.org/licenses/by-nc/3.0 2020 Guan et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c478t-86371e00061589865098a54a34618a592e8cf7487c4f0c3853c7fb14de0d80653 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work |
| ORCID | 0000-0003-3305-9108 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.2147/DMSO.S253920 |
| PMID | 32943895 |
| PQID | 2443670718 |
| PQPubID | 3933170 |
| PageCount | 26 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_38f66cb3d5484835bfc3ead362c0c1d9 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7455759 proquest_miscellaneous_2444380712 proquest_journals_2443670718 pubmed_primary_32943895 crossref_citationtrail_10_2147_DMSO_S253920 crossref_primary_10_2147_DMSO_S253920 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2020-01-01 |
| PublicationDateYYYYMMDD | 2020-01-01 |
| PublicationDate_xml | – month: 01 year: 2020 text: 2020-01-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | New Zealand |
| PublicationPlace_xml | – name: New Zealand – name: Macclesfield |
| PublicationTitle | Diabetes, metabolic syndrome and obesity |
| PublicationTitleAlternate | Diabetes Metab Syndr Obes |
| PublicationYear | 2020 |
| Publisher | Taylor & Francis Ltd Dove Dove Medical Press |
| Publisher_xml | – name: Taylor & Francis Ltd – name: Dove – name: Dove Medical Press |
| References | Sinzato (ref26) 2009; 55 Saez (ref14) 2003; 9 Si (ref34) 2007; 26 Zhang (ref19) 2020; 24 Sheedy (ref33) 2015; 6 Bevier (ref2) 1995; 24 ref1 Damasceno (ref27) 2011; 119 Rudge (ref23) 2007; 40 Dou (ref8) 2017; 41 de Souza Mda (ref25) 2010; 20 Angerstein (ref29) 2001; 109 Gare (ref4) 2008; 5 van de Bunt (ref7) 2013; 8 de Souza Mda (ref24) 2009; 18 Zhong (ref11) 2013; 56 Issemann (ref13) 1990; 347 Tomécarneiro (ref12) 2013; 72 Barak (ref15) 2002; 99 Wang (ref20) 2019; 9 Wu (ref9) 2016; 6 D’Anna (ref28) 2007; 64 Gabriely (ref31) 2008; 28 Mayer (ref30) 2010; 151 Janssen (ref36) 2015; 16 Tai (ref35) 2002; 22 Zhang (ref21) 2019; 7 Chakraborty (ref10) 2013; 14 Kim (ref32) 2012; 227 Bogacka (ref16) 2015; 53 Gimadiev (ref22) 2019; 64 Flynt (ref6) 2008; 9 Schaiff (ref17) 2006; 249 Barbour (ref3) 2007; 2 Solomon (ref5) 1997; 278 Zhou (ref18) 2019; 14 |
| References_xml | – volume: 151 start-page: 576 year: 2010 ident: ref30 publication-title: Endocrinology doi: 10.1210/en.2009-1122 – ident: ref1 – volume: 64 start-page: 723 year: 2019 ident: ref22 publication-title: Klin Lab Diagn doi: 10.18821/0869-2084-2019-64-12-723-729 – volume: 18 start-page: 562 year: 2009 ident: ref24 publication-title: Reprod Biomed Online doi: 10.1016/S1472-6483(10)60135-6 – volume: 119 start-page: 408 year: 2011 ident: ref27 publication-title: Endocrinol Diab – volume: 24 start-page: 793 year: 2020 ident: ref19 publication-title: Eur Rev Med Pharmacol Sci doi: 10.26355/eurrev_202001_20062 – volume: 8 start-page: e55272 year: 2013 ident: ref7 publication-title: PLoS One doi: 10.1371/journal.pone.0055272 – volume: 41 start-page: 2419 year: 2017 ident: ref8 publication-title: Cellular Physiol Biochem doi: 10.1159/000475912 – volume: 2 start-page: S112119 year: 2007 ident: ref3 publication-title: Diab Care doi: 10.2337/dc07-s202 – volume: 72 start-page: 69 year: 2013 ident: ref12 publication-title: Pharmacol Res doi: 10.1016/j.phrs.2013.03.011 – volume: 9 start-page: 406 year: 2019 ident: ref20 publication-title: 3 Biotech doi: 10.1007/s13205-019-1952-9 – volume: 6 start-page: 19 year: 2015 ident: ref33 publication-title: Front Immunol doi: 10.3389/fimmu.2015.00019 – volume: 56 start-page: 663 year: 2013 ident: ref11 publication-title: Diabetologia doi: 10.1007/s00125-012-2804-x – volume: 14 start-page: 119 year: 2019 ident: ref18 publication-title: Diagn Pathol doi: 10.1186/s13000-019-0899-9 – volume: 99 start-page: 303 year: 2002 ident: ref15 publication-title: Proc Nat Acad Sci doi: 10.1073/pnas.012610299 – volume: 227 start-page: 183 year: 2012 ident: ref32 publication-title: J Cell Physiol doi: 10.1002/jcp.22716 – volume: 20 start-page: 547 year: 2010 ident: ref25 publication-title: Reprod Biomed Online doi: 10.1016/j.rbmo.2010.01.002 – volume: 55 start-page: 384 year: 2009 ident: ref26 publication-title: Rev Assoc Med Bras doi: 10.1590/S0104-42302009000400010 – volume: 109 start-page: S135S148 year: 2001 ident: ref29 publication-title: Exp Clin Endocrinol Diab doi: 10.1055/s-2001-18576 – volume: 278 start-page: 1078 year: 1997 ident: ref5 publication-title: JAMA doi: 10.1001/jama.1997.03550130052036 – volume: 53 start-page: 189 year: 2015 ident: ref16 publication-title: Folia Histochem Cytobiol doi: 10.5603/fhc.a2015.0023 – volume: 40 start-page: 1095 year: 2007 ident: ref23 publication-title: Brazilian J Med Biol Res doi: 10.1590/S0100-879X2006005000132 – volume: 7 start-page: e1012 year: 2019 ident: ref21 publication-title: Mol Genet Genomic Med doi: 10.1002/mgg3.1012 – volume: 9 start-page: 831 year: 2008 ident: ref6 publication-title: Na .rev genet doi: 10.1038/nrg2455 – volume: 9 start-page: 1265 year: 2003 ident: ref14 publication-title: Nat Med doi: 10.1038/nm928 – volume: 28 start-page: 5369 year: 2008 ident: ref31 publication-title: Mol Cell Biol doi: 10.1128/MCB.00479-08 – volume: 64 start-page: 65 year: 2007 ident: ref28 publication-title: Gynecol Obstet Invest doi: 10.1159/000099149 – volume: 6 start-page: 1 year: 2016 ident: ref9 publication-title: Sci Rep doi: 10.1038/s41598-016-0001-8 – volume: 5 start-page: 1991 year: 2008 ident: ref4 publication-title: N Engl J Med – volume: 22 start-page: 805 year: 2002 ident: ref35 publication-title: Arterioscler Thromb Vasc Biol doi: 10.1161/01.ATV.0000012302.11991.42 – volume: 16 start-page: 760 year: 2015 ident: ref36 publication-title: Bmc Genomics doi: 10.1186/s12864-015-1969-3 – volume: 26 start-page: 2799 year: 2007 ident: ref34 publication-title: Oncogene doi: 10.1038/sj.onc.1210083 – volume: 249 start-page: 10 year: 2006 ident: ref17 publication-title: Mol Cell Endocrinol doi: 10.1016/j.mce.2006.02.009 – volume: 24 start-page: 103 year: 1995 ident: ref2 publication-title: Endocrinol Metab Clin North Am doi: 10.1016/S0889-8529(18)30055-0 – volume: 14 start-page: 1110 year: 2013 ident: ref10 publication-title: Curr Drug Targets doi: 10.2174/13894501113149990182 – volume: 347 start-page: 645 year: 1990 ident: ref13 publication-title: Nature doi: 10.1038/347645a0 |
| SSID | ssj0000070422 |
| Score | 2.3143532 |
| Snippet | This study aimed to investigate the role of
expression in the reduction of placental function in GDM patients.
qRT-PCR was used to detect the differential... Purpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients. Materials and Methods: qRT-PCR... This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.PURPOSEThis study aimed to investigate the... Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 3009 |
| SubjectTerms | Acids Cell culture Family planning Gene expression Gestational diabetes gestational diabetes mellitus Glucose Hybridization Insulin resistance Laboratory animals Maternal & child health Metabolism MicroRNAs mirna-21 Original Research ppar-α Pregnancy Womens health |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwEB2hHhAXxDeBgowEJ2SaxE7sHEtLqZAWVlsq9RYl_lAjlSxis1f-E3-E38SM7a52EYgLt914Dk5mHL8ZT94DeFkLTLm8y7mz0nBZm4Jr3GhDtcl3RpvGhgbZj_XpufxwUV1sSX1RT1ikB44P7kBoX9emFxahtUS40Hsj8O7xvWtyU9jw6V7e5FvJVAS-isitorKK5gr_x653kuU5OJ6dfXpzVlaIDfKd_SjQ9v8Ja_7eMrm1B53cgdsJPLLDOOm7cMON9-DmLB2P34fvx5hT80WUl3eWfRkWvCzYMLL3bjWlsh9LTTArNiMyzmm9YnOqpY9Tx0jIA18cbD4_XPCfP9i0xEuXQz9M7AiN2Zw0fryLUcO60eKwH64S9e4DOD959_nolCeBBW6k0hPXtVCFi7BGN5rI9HRXyU7IusAfTem08QpTGiN9bgTu7Eb5vpDW5ZYOZMVD2BuXo3sMzEhMrBqEm5VW0rkS3YxgpOkxgbSVsD6D19ePuTWJfZxEMK5azELIKS05pU1OyeDVxvprZN34i91b8tjGhriywwWMoDZFUPuvCMpg_9rfbVrAqxZRD1Hb4c6dwYvNMC49Ok_pRrdcB5vA11-UGTyK4bGZiSgb0pWvMlA7gbMz1d2RcbgM9N5KVqSa-uR_3NtTuFVSgSDUjPZhb_q2ds8QRU3987BgfgHdzBhW priority: 102 providerName: Directory of Open Access Journals – databaseName: Public Health Database dbid: 8C1 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagSIgL4k2gVEaCEzJNbCdxTlW7pVRIC6stlXqLEj9opJKUJnvlP_FH-E3MON6URcAtiudgZWY8D0--j5BXmYCSy9mYWSM1k5lOmIJA67tNrtJKF8YPyH7Mjk_lh7P0LDTc-jBWuT4T_UFtOo098l0IQ4g1Bkfp3uU3hqxReLsaKDRuklv4DygWX2qWTD0WxLKRnI_z7kjIs3s4P_n09oSnkBXEG5HIA_b_Lcv8c1jyt-hzdI_cDWkj3R_1fJ_csO0DcnseLsYfku-HUE2z5Ugsbw392iwZT2jT0ve2H0LDj4bxl57OEYZzWPV0gV30dqgoUnjAkUEXi_0l-_mDDh28Om_qZqAzEKYLZPdxdrQXWrUGll1zEUB3H5HTo3efZ8csUCswLXM1MJWJPLFjQqMKhTB6qkplJWSWwEPBrdIuh2JGSxdrATFd565OpLGxwatY8ZhstV1rnxKqJZRUBSSaqcqltRwUDGlIUUPpaFJhXETerD9zqQPuONJfXJRQf6BSSlRKGZQSkdeT9OWIt_EPuQPU2CSDKNn-RXf1pQxOVwrlskzXwkBZJiHVrJ0W4DkQs3WsE1NEZHut7zK4bl9eG1pEXk7L4HR4k1K1tlt5GY_Un_CIPBnNY9qJ4AUyyqcRyTcMZ2Ormyttc-6BvXOZIl_qs_9v6zm5w7Ho932gbbI1XK3sC8iMhnrHm_8v7EgNSA priority: 102 providerName: ProQuest |
| Title | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32943895 https://www.proquest.com/docview/2443670718 https://www.proquest.com/docview/2444380712 https://pubmed.ncbi.nlm.nih.gov/PMC7455759 https://doaj.org/article/38f66cb3d5484835bfc3ead362c0c1d9 |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1bixMxFA7rLogv4t3RtUTQJ8namWQmmQeR3e4NoWvpWujbMJOLO1Cn2k5BX_xP_hF_k-dk0mJ1BV_KNAllyMnp-c7J4fsIeZFxSLmc7TNrhGYi0zFTEGh9tcmVWunc-AbZi-x8It5N0-kOWauNhg1cXpvaoZ7UZDE7-Prl21tw-DfYxhwL-fp4ePn-4DJJIdRD8r7nb4qwiS8A_Q4ISyS76pRWFJPwveuC_-sHtuKTp_G_Dnv-2UL5W0w6vUNuBzBJDzvr3yU7trlHbg7Ddfl98v0Ycmw27uTmraGf6jFLYlo39Mwu21AGpKEpZkmHSM7ZrpZ0hLX1pi0pCnvAHwkdjQ7H7OcP2s5h6Kqu6pYOYDEdoeaPs90pomVjYNrVs0DF-4BMTk8-DM5ZEFxgWkjVMpVxGdsO5qhcIbmeKlNRcpHF8JAnVmknIcXRwvU1h0ivpatiYWzf4AUtf0h2m3ljHxOqBSRaOcDPVElhbQJmB3CSV5BQmpQbF5FX620udGAjR1GMWQFZCRqlQKMUwSgReblZ_blj4fjHuiO02GYNcmf7gfniYxFcseDKZZmuuIFkTQAArZzm4E8QyXVfxyaPyP7a3sX6PBaAgpDqDiJ5RJ5vpsEV8X6lbOx85dd4_v44icij7nhs3oQnOerMpxGRWwdn61W3Z5r6ytN9S5GiiuqT_9yDp-RWgjUBXybaJ7vtYmWfAXBqqx65oQZxj-wdnVyMxj1ffoDPsymMobf8AlkqGGU |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB6VIgEXxJtAASPREwpNHCdxDgiVXcqWdstq20q9pYnt0EglKU1WiAs_CYk_wm9iJi9YBNx6W8WjlZWZ8Tw8-T6AZ4GHJVdmHNtooWwRKNeWGGibblOWKKki3QzI7gWTQ_HuyD9agW_9tzA0Vtmfic1BrUtFPfINDEOENYZH6auzTzaxRtHtak-h0ZrFjvnyGUu26uX2GPW7zvnWm4PRxO5YBWwlQlnbMvBC17SxXEaSEORk4ovEE4GLPyJupMpCzOOVyBzlYThTYZa6QhtH0y2kh_97CS7Tl0aE1S9H7tDTIewcwXk7X08EQBvj6f77F_vcxyzEWYp8DUHA37LaP4czf4t2Wzfgepemss3Wrm7CiiluwZVpdxF_G76OsXq35y2RvdHsYz63ucvygr01Vd01GFk3blOxKcF-1ouKzahrX9QJI8oQPKLYbLY5t398Z3WJj07yNK_ZCIXZjNiEMtPaJ0sKjctZftqB_N6Bwwt56XdhtSgLcx-YEljCRZjY-jIUxnA0KEx7ohRLVe17OrPgef-aY9XhnBPdxmmM9Q4pJSalxJ1SLFgfpM9afI9_yL0mjQ0yhMrdPCjPP8Sdk8eezIJApZ7GMlBgaptmykNPxRxBOcrVkQVrvb7j7qio4l-GbcHTYRmdnG5uksKUi0amYQZwuQX3WvMYduLxiBjsfQvCJcNZ2urySpGfNEDiofCJn_XB_7f1BK5ODqa78e723s5DuMap4dD0oNZgtT5fmEeYldXp48YVGBxftO_9BI_9Ryc |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VVqq4IN4YCiwSPSETe71-HSrUNg0tJcFKqdSba--DWipOaRwhLvwn_gYHfhMz9iYQBNx6i7yjaOWZndeOvw_geRRgyWW052olpCsi6bsJBtq222QKmchUtQOyo2j_WLw5CU9W4Pv8Wxgaq5z7xNZRq4mkHnkPwxBhjaEr7Rk7FpH1B68uPrnEIEU3rXM6jcLSLKitFm7MfuRxqL98xnJuunXQR91vcj7Ye7-771rGAVeKOGncJApiX3dxPkkTQpdLilAUgYh8_JFynUgTY44vhfFkgKFOxqb0hdKeohvKAP_3GqzFGPWxEFzb2Rtl40XHh5B1BOfd9D3RA_X6w6N3L494iDmKtxQXW_qAv-W8f45u_hYLBzfhhk1i2XZndbdgRde3YX1or-nvwNc-1vbuuKO514p9rMYu91lVs9d62tj2I7PDOFM2JFDQZjZlGfX066ZgRCiCDoxl2fbY_fGNNRN8dFaVVcN2UZhlxDVkdGe9rKgVLpvq3EIA34XjK3nt92C1ntT6ATApsMBLMe0Nk1hozdHcMClKSyxkVRgo48CL-WvOpUVBJzKO8xyrIVJKTkrJrVIc2FxIX3ToH_-Q2yGNLWQIs7t9MLn8kFsXkAeJiSJZBgqLRIGJb2lkgOcYMwjpSV-lDmzM9Z1bRzLNf5m9A88Wy-gC6F6nqPVk1sq0vAE-d-B-Zx6LnQQ8JX770IF4yXCWtrq8UldnLcx4LEJib334_209hXU8h_nbg9HhI7jOqRvRNqg2YLW5nOnHmLI15RN7FhicXvXx-wk8jlI3 |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Down-Regulated+miR-21+in+Gestational+Diabetes+Mellitus+Placenta+Induces+PPAR-%CE%B1+to+Inhibit+Cell+Proliferation+and+Infiltration&rft.jtitle=Diabetes%2C+metabolic+syndrome+and+obesity&rft.au=Guan%2C+Chun-Yi&rft.au=Tian%2C+Shi&rft.au=Cao%2C+Jing-Li&rft.au=Wang%2C+Xue-Qin&rft.date=2020-01-01&rft.issn=1178-7007&rft.eissn=1178-7007&rft.volume=13&rft.spage=3009&rft.epage=3034&rft_id=info:doi/10.2147%2FDMSO.S253920&rft.externalDBID=n%2Fa&rft.externalDocID=10_2147_DMSO_S253920 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1178-7007&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1178-7007&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1178-7007&client=summon |