Antibody Libraries as Tools to Discover Functional Antibodies and Receptor Pleiotropism

Most antibodies currently in use have been selected based on their binding affinity. However, nowadays, antibodies that can not only bind but can also alter the function of cell surface signaling components are increasingly sought after as therapeutic drugs. Therefore, the identification of such fun...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 22; no. 8; p. 4123
Main Authors Lin, Chih-Wei, Lerner, Richard A.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 16.04.2021
MDPI
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ISSN1422-0067
1661-6596
1422-0067
DOI10.3390/ijms22084123

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Summary:Most antibodies currently in use have been selected based on their binding affinity. However, nowadays, antibodies that can not only bind but can also alter the function of cell surface signaling components are increasingly sought after as therapeutic drugs. Therefore, the identification of such functional antibodies from a large antibody library is the subject of intensive research. New methods applied to combinatorial antibody libraries now allow the isolation of functional antibodies in the cellular environment. These selected agonist antibodies have provided new insights into important issues of signal transduction. Notably, when certain antibodies bind to a given receptor, the cell fate induced by them may be the same or different from that induced by natural agonists. In addition, combined with phenotypic screening, this platform allows us to discover unexpected experimental results and explore various phenomena in cell biology, such as those associated with stem cells and cancer cells.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22084123